Lim, Po-Teen
Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

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OCCURRENCE OF PSEUDO-NITZSCHIA SPECIES (BACILLARIOPHYCEAE) AT KUCHING ESTUARIES FROM YEAR 2007 TO 2010 Lim, Po-Teen
Marine Research in Indonesia Vol 37, No 2 (2012)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1408.734 KB) | DOI: 10.14203/mri.v37i2.27

Abstract

Amnesic shellfish poisoning (ASP) is a type of seafood poisoning due to human consumption on shellfish mollusks contaminated with algal toxin, domoic acid (DA) derived from the diatom Pseudo-nitzschia. Bloom dynamics of this diatom in tropical waters were poorly understood due to lack of long term study on the organism. Occurrence of Pseudo-nitzschia species was investigated at Santubong and Samariang estuarine waters, Kuching, Sarawak from 2007 to 2010. The estuaries were characterized by shallow water with semi-diurnal tidal cycle. Cell abundance was determined by microscopic enumeration. Temperature, pH, salinity and macronutrients at the sampling sites were determined at each sampling occasions. Highest cell density of Pseudo-nitzschia spp. was recorded 8.0 × 103 cells L in Santubong in April 2009 and P. brasiliana cells were found highest in October 2007 (2 × 104 cells L-1). Increase in cell abundance was coincided with high salinity and low precipitation rate. Water temperature and pH showed insignificant influence on the abundance of Pseudo-nitzschia spp. The result obtained in this study provided fundamental understanding on bloom dynamic of potential harmful Pseudo-nitzschia species in the tropical estuarine waters.
BENTHIC HARMFUL DINOFLAGELLATE ASSEMBLAGES IN A FRINGING REEF OF SAMPADI ISLAND, SARAWAK, MALAYSIA Tan, Toh-Hii; Lim, Po-Teen; Mujahid, Aazani; Usup, Gires; Leaw, Chui-Pin
Marine Research in Indonesia Vol 38, No 2 (2013)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4990.221 KB) | DOI: 10.14203/mri.v38i2.59

Abstract

A study on the presence and relative abundance of benthic harmful algal bloom (BHAB) forming dinoflagellate species was carried out in the coral reefs of Sampadi Island, Sarawak, Malaysia. The study involved deployment of fiberglass screens as an artificial substrate for the benthic epiphytic microalgae. The screens were placed for 24 h above the seafloor along a 100 m transect at 10 m intervals. BHAB species attached to the screens were identified and cell abundances were enumerated under a light microscope. The BHAB community at the study site was dominated by Prorocentrum spp. and Coolia spp. Other BHAB species collected included Amphidinium spp., Gambierdiscus spp. and Ostreopsis spp. Total cell densities collected on the screens ranged from 5 to 100 cells per 100 cm2. The two BHAB groups of primary concern, Gambierdiscus spp. and Ostreopsis spp. were detected at relatively low abundances of 0.6–4.2% and 1.8–16% respectively. This study has shown that potentially toxic BHAB species were present in the coral reef and the artificial substrate approach could provide a convenient quantitative method for the collection of clean samples for identification and enumeration purposes.
GROWTH RESPONSES OF FIVE NON TOXIC ALEXANDRIUM SPECIES (DINOPHYCEAE) TO TEMPERATURE AND SALINITY Lim, Po-Teen; Leaw, Chui-Pin; Kaga, Shinnosuke; Sekiguchi, Katsushi; Ogata, Takehiko
Marine Research in Indonesia Vol 32, No 2 (2007)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7932.983 KB) | DOI: 10.14203/mri.v32i2.454

Abstract

Growth response of five clonal cultures of Alexandrium obtained from tropical and temperate waters were examined. Experiments were carried out in eighteen variable temperature-salinity conditions (temperatures of 15 °C, 20°C, and 25°C; salinities between 5 to 30 psu) under constant illumination of 150 ± 10.0 Amol m-2 s- at 15:9 light:dark photo-cycle. Our results showed optimum growth of all Alexandrium species at 20 - 25°C. The salinity range for optimum growth however varied among the species. Growth rates of A. eine, A. insuetum, and A. fraterculus (0.28 — 0.37 day) were higher than those of A. leei and A. pseudogoniaulax under the same culture conditions (0.14 —0.22 day-). The three temperate species showed positive growth at suboptimum temperature, 15°C, but the tropical species did not grow and died off. Salinity tolerance of the five species in decreasing order was A. pseudogoniaulax > A. leei > A. insuetum > A. affine > A. fraterculus. Results of the present study showed vast variations in salinity tolerance among the Alexandrium species regardless the geographical origins. Adaptation of the temperate species at higher temperature indicated that the species might proliferate in warm tropical waters.