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<![CDATA[BENTHIC HARMFUL DINOFLAGELLATE ASSEMBLAGES IN A FRINGING REEF OF SAMPADI ISLAND, SARAWAK, MALAYSIA ]]>
<![CDATA[Tan, Toh-Hii]]>;
<![CDATA[Lim, Po-Teen]]>;
<![CDATA[Mujahid, Aazani]]>;
<![CDATA[Usup, Gires]]>;
<![CDATA[Leaw, Chui-Pin]]>
<![CDATA[ Marine Research in Indonesia Vol 38, No 2 (2013) ]]>
Publisher : <![CDATA[Research Center for Oceanography - Indonesian Institute of Sciences (LIPI) ]]>
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DOI: 10.14203/mri.v38i2.59
<![CDATA[A study on the presence and relative abundance of benthic harmful algal bloom (BHAB) forming dinoflagellate species was carried out in the coral reefs of Sampadi Island, Sarawak, Malaysia. The study involved deployment of fiberglass screens as an artificial substrate for the benthic epiphytic microalgae. The screens were placed for 24 h above the seafloor along a 100 m transect at 10 m intervals. BHAB species attached to the screens were identified and cell abundances were enumerated under a light microscope. The BHAB community at the study site was dominated by Prorocentrum spp. and Coolia spp. Other BHAB species collected included Amphidinium spp., Gambierdiscus spp. and Ostreopsis spp. Total cell densities collected on the screens ranged from 5 to 100 cells per 100 cm2. The two BHAB groups of primary concern, Gambierdiscus spp. and Ostreopsis spp. were detected at relatively low abundances of 0.6ââ¬â4.2% and 1.8ââ¬â16% respectively. This study has shown that potentially toxic BHAB species were present in the coral reef and the artificial substrate approach could provide a convenient quantitative method for the collection of clean samples for identification and enumeration purposes.]]>
<![CDATA[MOLECULAR PHYLOGENY OF LEIOGNATHIDAE IN THE WATERS OF PERHENTIAN ISLANDS, TRENGGANU, MALAYSIA ]]>
<![CDATA[Giat, Seah Ying]]>;
<![CDATA[Ghaffar, Mazlan Abd.]]>;
<![CDATA[Usup, Gires]]>
<![CDATA[ Marine Research in Indonesia Vol 33, No 2 (2008) ]]>
Publisher : <![CDATA[Research Center for Oceanography - Indonesian Institute of Sciences (LIPI) ]]>
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DOI: 10.14203/mri.v33i2.487
<![CDATA[Several series of trawl surveys were carried out in the waters of Perhentian Islands using commercial trawlers. 16S mitochondrial rRNA gene sequences were used to infer the phylogenetic relationships among nine morphospecies of leiognathids. The results showed that the genus Leiognathus is paraphyletic, whereas Gazza secutor, Photoplagios, Photopectoralis and Nuchequula are monophyletic. The molecular phylogenetic posions of the leiognathids studied were identical with morphological delineation, except for Photoplagios stercorarius. Branch of P. stercorarius was placed between Photoplagios spp. clade and clade of Secutor and Gazza. P. stercorarius was more affiliated to genus Photoplagios morphologically however have slight different features of light organ system compared with others three Photoplagios sp. caught in this study. It is probable that two distinct subclades occur in genus Photoplagios. Leiognathus equulus formed the base of the other leiognathids. Leiognathus splendens and Leiognathus jonesi formed a sister taxa to Photopectoralis species. Gazza formed a sister taxa to Secutor and Nuchequula formed a sister taxa to the group of trifurcation but toth with low bootstrap support. This study has shown that 16S mitochondrial rDNA is a good marker for phylogenetic analysis of the Leiognathidae. ]]>
<![CDATA[BENTHIC HARMFUL DINOFLAGELLATE ASSEMBLAGES IN A FRINGING REEF OF SAMPADI ISLAND, SARAWAK, MALAYSIA ]]>
<![CDATA[Tan, Toh-Hii]]>;
<![CDATA[Lim, Po-Teen]]>;
<![CDATA[Mujahid, Aazani]]>;
<![CDATA[Usup, Gires]]>;
<![CDATA[Leaw, Chui-Pin]]>
<![CDATA[ Marine Research in Indonesia Vol 38 No 2 (2013) ]]>
Publisher : <![CDATA[Research Center for Oceanography - Indonesian Institute of Sciences (LIPI) ]]>
Show Abstract
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Full PDF (4990.221 KB)
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DOI: 10.14203/mri.v38i2.59
<![CDATA[A study on the presence and relative abundance of benthic harmful algal bloom (BHAB) forming dinoflagellate species was carried out in the coral reefs of Sampadi Island, Sarawak, Malaysia. The study involved deployment of fiberglass screens as an artificial substrate for the benthic epiphytic microalgae. The screens were placed for 24 h above the seafloor along a 100 m transect at 10 m intervals. BHAB species attached to the screens were identified and cell abundances were enumerated under a light microscope. The BHAB community at the study site was dominated by Prorocentrum spp. and Coolia spp. Other BHAB species collected included Amphidinium spp., Gambierdiscus spp. and Ostreopsis spp. Total cell densities collected on the screens ranged from 5 to 100 cells per 100 cm2. The two BHAB groups of primary concern, Gambierdiscus spp. and Ostreopsis spp. were detected at relatively low abundances of 0.6â??4.2% and 1.8â??16% respectively. This study has shown that potentially toxic BHAB species were present in the coral reef and the artificial substrate approach could provide a convenient quantitative method for the collection of clean samples for identification and enumeration purposes.]]>
<![CDATA[MOLECULAR PHYLOGENY OF LEIOGNATHIDAE IN THE WATERS OF PERHENTIAN ISLANDS, TRENGGANU, MALAYSIA ]]>
<![CDATA[Giat, Seah Ying]]>;
<![CDATA[Ghaffar, Mazlan Abd.]]>;
<![CDATA[Usup, Gires]]>
<![CDATA[ Marine Research in Indonesia Vol 33 No 2 (2008) ]]>
Publisher : <![CDATA[Research Center for Oceanography - Indonesian Institute of Sciences (LIPI) ]]>
Show Abstract
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Download Original
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Full PDF (6407.13 KB)
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DOI: 10.14203/mri.v33i2.487
<![CDATA[Several series of trawl surveys were carried out in the waters of Perhentian Islands using commercial trawlers. 16S mitochondrial rRNA gene sequences were used to infer the phylogenetic relationships among nine morphospecies of leiognathids. The results showed that the genus Leiognathus is paraphyletic, whereas Gazza secutor, Photoplagios, Photopectoralis and Nuchequula are monophyletic. The molecular phylogenetic posions of the leiognathids studied were identical with morphological delineation, except for Photoplagios stercorarius. Branch of P. stercorarius was placed between Photoplagios spp. clade and clade of Secutor and Gazza. P. stercorarius was more affiliated to genus Photoplagios morphologically however have slight different features of light organ system compared with others three Photoplagios sp. caught in this study. It is probable that two distinct subclades occur in genus Photoplagios. Leiognathus equulus formed the base of the other leiognathids. Leiognathus splendens and Leiognathus jonesi formed a sister taxa to Photopectoralis species. Gazza formed a sister taxa to Secutor and Nuchequula formed a sister taxa to the group of trifurcation but toth with low bootstrap support. This study has shown that 16S mitochondrial rDNA is a good marker for phylogenetic analysis of the Leiognathidae.]]>
<![CDATA[Studi ketoksikan dinoflagelata spesies Prorocentrum minimum (Dinophyceae) Schiller (Pavillard) ]]>
<![CDATA[., Rozirwan]]>;
<![CDATA[Usup, Gires]]>
<![CDATA[ Maspari Journal : Marine Science Research Vol 1, No 1 (2010): Edisi Juli ]]>
Publisher : <![CDATA[UNIVERSITAS SRIWIJAYA ]]>
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DOI: 10.36706/maspari.v1i1.1030
<![CDATA[This study was carried out to determine the toxicity of the dinoflagellate Prorocentrum minimum species that formed massive blooms in Lido Beach, Johor Bahru in July 2002. Clone cultures were established in ES-DK medium at 26o C under a 14:10 hour light dark cycle. Species toxicity was determined by intra peritoneal (i.p) injection of culture extract into mice. Cultured cell extracts were toxic to mice. The major symptoms were muscular paralysis and diarrhea. However no mouse mortality was observed even after 13 hours. Extracts of cultured cells were also hemolytic on rabbit red blood cells. Keywords: Dinoflagellate, HAB, Prorocentrum minimum, Red Tide dan Toxicity Kajian dilakukan untuk menentukan ketoksikan dinoflagelata spesies Prorocentrum minimum yang menyebabkan kejadian pasang merah besar-besaran di perairan Pantai Lido, Johor Bahru pada Juli tahun 2002. Kultur klon telah dibuat dalam medium ES-DK pada suhu 26o C dan siklus pencahayaan 14:10 jam terang gelap. Ketoksikan spesies diuji dengan penyuntikan ekstrak kultur secara intra peritoneal (i.p.) terhadap tikus. Hasil pengamatan menunjukan ekstrak sel yang dikultur memberikan pengaruh racun kepada tikus. Gejala utama adalah kejang otot dan menceret/diare. Walau bagaimanapun tidak didapati tikus yang mati setelah dilakukan pengamatan selama 13 jam. Ekstrak toksin spesies ini juga menunjukkan pengaruh hemolitik terhadap eritrosit kelinci. Kata kunci: Dinoflagellata, HAB, Prorocentrum minimum, Red Tide dan Toxicity]]>
<![CDATA[SCREENING OF TOXIC MARINE NITZSCHIA SPECIES (BACILLARIOPHYCEAE) IN MALAYSIA ]]>
<![CDATA[Suriyanti Su Nyun Pau]]>;
<![CDATA[Dzulhelmi Muhammad Nasir]]>;
<![CDATA[Gires Usup]]>
<![CDATA[ Jurnal Kelautan Vol 10, No 1 (2017) ]]>
Publisher : <![CDATA[Department of Marine Sciences, Trunojoyo University of Madura, Indonesia ]]>
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DOI: 10.21107/jk.v10i1.2635
<![CDATA[Amnesic Shellfish Poisoning (ASP) is a type of intoxication caused by the neurotoxin domoic acid (DA). The diatom genus Nitzschia is capable of producing this toxin. Screening for the presence of toxic Nitzschia spp. was carried out at various estuaries in Malaysia. Nitzschia-like cells were isolated and established into clonal cultures. Late stationary phase of cultures were harvested and tested for toxin production using HPLC. Toxin production and compound was verified by LC-MS. From the analyses, at least three cultures were detected with DA, while the rest of the cultures did not show detectable amounts of DA. The localities of the toxic species are Johor and Sabah. Here we conclude that toxic Nitzschia species are present in Malaysian water.Keywords: diatom, estuary, intoxication, safety, toxic ]]>
<![CDATA[Studi ketoksikan dinoflagelata spesies Prorocentrum minimum (Dinophyceae) Schiller (Pavillard) ]]>
<![CDATA[Rozirwan .]]>;
<![CDATA[Gires Usup]]>
<![CDATA[ Maspari Journal : Marine Science Research Vol 1, No 1 (2010): Edisi Juli ]]>
Publisher : <![CDATA[UNIVERSITAS SRIWIJAYA ]]>
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Full PDF (277.835 KB)
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DOI: 10.36706/maspari.v1i1.1030
<![CDATA[This study was carried out to determine the toxicity of the dinoflagellate Prorocentrum minimum species that formed massive blooms in Lido Beach, Johor Bahru in July 2002. Clone cultures were established in ES-DK medium at 26o C under a 14:10 hour light dark cycle. Species toxicity was determined by intra peritoneal (i.p) injection of culture extract into mice. Cultured cell extracts were toxic to mice. The major symptoms were muscular paralysis and diarrhea. However no mouse mortality was observed even after 13 hours. Extracts of cultured cells were also hemolytic on rabbit red blood cells. Keywords: Dinoflagellate, HAB, Prorocentrum minimum, Red Tide dan Toxicity Kajian dilakukan untuk menentukan ketoksikan dinoflagelata spesies Prorocentrum minimum yang menyebabkan kejadian pasang merah besar-besaran di perairan Pantai Lido, Johor Bahru pada Juli tahun 2002. Kultur klon telah dibuat dalam medium ES-DK pada suhu 26o C dan siklus pencahayaan 14:10 jam terang gelap. Ketoksikan spesies diuji dengan penyuntikan ekstrak kultur secara intra peritoneal (i.p.) terhadap tikus. Hasil pengamatan menunjukan ekstrak sel yang dikultur memberikan pengaruh racun kepada tikus. Gejala utama adalah kejang otot dan menceret/diare. Walau bagaimanapun tidak didapati tikus yang mati setelah dilakukan pengamatan selama 13 jam. Ekstrak toksin spesies ini juga menunjukkan pengaruh hemolitik terhadap eritrosit kelinci. Kata kunci: Dinoflagellata, HAB, Prorocentrum minimum, Red Tide dan Toxicity]]>
<![CDATA[Contribution to the current list of Nitzschia species (Bacillariophyceae) in Malaysia ]]>
<![CDATA[Pau, Suriyanti Su Nyun]]>;
<![CDATA[Nasir, Dzulhelmi Muhammad]]>;
<![CDATA[Usup, Gires]]>
<![CDATA[ Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Jurnal Ilmu Perairan, Vol. 8: No. 2 (August, 2021) ]]>
Publisher : <![CDATA[Universitas Malikussaleh ]]>
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DOI: 10.29103/aa.v8i2.4777
<![CDATA[Current occurrence of Nitzschia Hassall from marine and freshwater environment in Malaysia is compiled from current and previously published materials. Phytoplankton was collected using a 20 µm plankton net from waterfronts of Peninsular Malaysia and Borneo. Some Nitzschia cells were successfully isolated and established into clonal cultures. For morphology observation, organic matters of the net samples and cultures were oxidized and removed through acid treatment. Cleaned specimens were observed using light and electron microscopes. The morphology of the Nitzschia cells was described and micrographs are included. In addition to current field sampling, Nitzschia spp. records and distributions in Malaysia had also been compiled from secondary data. Sources of references whereby the Nitzschia species were listed in this article were cited accordingly. This article compiles a total of 11 Nitzschia spp. found in Malaysia including a few revisions and new records. This article could serve as an important reference for Nitzschia taxonomic and distributional study particularly in tropical region.Keywords: distribution, morphology, taxonomy, tropical]]>
