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All Journal Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Herbal Medicine Journal Jurnal Pharmactive
Puspita Sari, Dyah Ratna Ayu
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Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Other

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POTENSI ANDROGRAFOLID, DEOKSIANDROGRAFOLID, NEOANDROGRAFOLID SEBAGAI ANTIATEROSKLEROSIS PADA CASPASE-1: STUDI IN SILICO Ugrasena, Putu Yudha; Puspita Sari, Dyah Ratna Ayu; Indraswari, Putu Ika Indah
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 9 No 2 (2024): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jiis.v9i2.1625

Abstract

Atherosclerosis is a vascular pathological condition characterized by active, chronic, progressive inflammation and plaque formation in the arterial walls Interleukin 1-beta (IL-1β) cytokine influences every stage of atherosclerosis, from destroying monocytes and other immune cells to forming plaques. Caspase-1 protein plays a role in activating the inflammatory cytokine Pro-Interleukin-1 Beta (Pro IL-1β) to IL-1β. One of the plants that has the potential to treat atherosclerosis is Andrographis paniculata. Andrographolide and its analogs, such as neoandrographolide and deoxyandrographolide, are the main bioactive compounds with various pharmacological activities. This study aimed to screen the activity of the compounds through the description of the stability of the compounds in protein caspase-1. This type of research was an in-silico exploratory study of the caspase-1 protein (PDB ID: 1RWK), with compound preparations, protein preparations, validating the molecular docking method to docking andrographolide neoandrografolid and deoksiandrografolid compounds on the target protein. The compounds has an affinity for the target protein with binding energy values ​​of -5.95 kcal/mol, -6.02 kcal/mol, and -6.81 kcal/mol respectively, which are smaller than the native ligand -4.77 kcal/mol. Docking results show that andrographolide, neoandrografolid, and deoxyandrografolid can potentially inhibit caspase-1, which activates the pro-inflammatory cytokine IL-1β in atherosclerosis.
UJI TOKSISITAS EKSTRAK ETANOL 96% DAUN MIANA (Plectranthus scutellaroides R.Br) TERHADAP Artemia salina DENGAN METODE BSLT (Brine Shrimp Lethality Test) Puspita Sari, Dyah Ratna Ayu; Putu Yudha Ugrasena; Ni Putu Ayu Desi Astini; I Gede Agus Sindhu A
Journal Pharmactive Vol. 2 No. 2 (2023): Jurnal Pharmactive Oktober
Publisher : Institut Teknologi dan Kesehatan Bintang Persada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64036/pharmactive.v2i2.34

Abstract

Medicinal plants are one of the efforts used to control disease. The benefits of medicinal plants have been passed down from generation to generation by our ancestors. One plant that has medicinal properties is miana leaves (Plectranthus scutellaroides R.Br). In this research, the extraction method used was maceration, with 96% ethanol solvent. Miana leaf ethanol extract was screened for phytochemicals and toxicity tested using the Brine Shrimp Lethality Test (BSLT) method. Toxicity tests were carried out at various concentrations of 100 ppm, 250 ppm, 500 ppm, 750 ppm and 1000 ppm. The results of phytochemical screening on miana leaves show that miana leaves contain flavonoid compounds, tannins, saponins, steroids, but do not contain alkaloids and triterpenoids. The 96% ethanol extract of miana leaves (Plectranthus scutellaroides R.Br) has an LC50 value of 511.58 ppm, included in the toxic category with an LC50 value in the range of 30-1000 ppm.
UJI TOKSISITAS EKSTRAK ETANOL 96 % DAUN CINCAU HIJAU (Cyclea barbata Miers) TERHADAP Artemia salina Leach. DENGAN METODE Brine Shrimp Lethality Test (BSLT) Puspita Sari, Dyah Ratna Ayu
Journal Pharmactive Vol. 3 No. 1 (2024): Jurnal Pharmactive April
Publisher : Institut Teknologi dan Kesehatan Bintang Persada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64036/pharmactive.v3i1.47

Abstract

Medicinal plants are one of the efforts used to control disease. The benefits of medicinal plants have been passed down from generation to generation by our ancestors. One plant that has medicinal properties is green grass jelly (Cyclea barbata Miers). In this research, the extraction method used was maceration, with 96% ethanol solvent. The ethanol extract of green grass jelly leaves was screened for phytochemicals and tested for toxicity using the Brine Shrimp Lethality Test (BSLT) method. Toxicity tests were carried out at various concentrations of 100 ppm, 250 ppm, 500 ppm, 750 ppm and 1000 ppm. The results of the analysis of secondary metabolites contained in the ethanol extract of green grass jelly leaves are alkaloids, flavonoids, tannins and steroids. In the toxicity test, the highest average death percentage was obtained at the 1000 ppm extract concentration variation of 73.33%. From the probit and log concentration data, a linear regression equation y = was obtained with an LC 50 value of 558,25 ppm. This shows that the 96% ethanol extract of green grass jelly leaves (Cyclea barbata Miers) has toxic potential because the LC 50 value is in the range of 30-1000 ppm.
Pengaruh Perbedaan Ketinggian Tumbuh Daun Miana (Coleus scutellarioides) di Daerah Bali Terhadap Kadar Flavonoid Total Puspita Sari, Dyah Ratna Ayu; Ugrasena, Putu Yudha; Indraswari, Putu Ika Indah
Herbal Medicine Journal Vol 8 No 2 (2025): Herbal Medicine Journal
Publisher : Program Studi S1 Farmasi, STIKES Senior, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58996/hmj.v8i2.156

Abstract

Miana (Coleus scutellarioides) contains flavonoids as its primary active components and is widely used in traditional medicine, particularly in Indonesia. In Bali, which is renowned for its traditional healing practices (Usada Bali), miana is commonly utilized as a herbal remedy. The leaves of miana possess various pharmacological activities, including anti-inflammatory, antiproliferative, antidiabetic, anthelmintic, and antipyretic effects. Geographical conditions, such as the altitude of the growing region, are among the factors that influence the flavonoid content in miana leaves. This study aimed to determine the total flavonoid content in miana leaves from three different altitudes in Bali: Bedugul (1273 masl), Klungkung (393 masl), and Sanur (11 masl). An experimental laboratory design was employed, using maceration with 96% ethanol as the extraction method. The total flavonoid content was quantified using UV-Vis spectrophotometry, with quercetin as the reference standard. The results showed that miana leaves from Klungkung exhibited the highest total flavonoid content (540.85 ± 7.97 mgQE/g), followed by Bedugul (488.68 ± 6.07 mgQE/g) and Sanur (368.53 ± 11.2 mgQE/g). Statistical analysis revealed that the total flavonoid content of miana leaves from Klungkung was significantly higher (P<0.05) than that from the other two regions. These findings indicate that the altitude of the growing region affects the total flavonoid content in miana leaves, with the highest levels observed at an intermediate altitude (393 masl).
Ethanolic Extract of Cyclea barbata Leaves as a Promising Natural Candidate for Anti Peptic Ulcer: Ekstrak Etanol Daun Cyclea barbata sebagai Kandidat Alami Antitukak Lambung Puspita Sari, Dyah Ratna Ayu; Ugrasena, Putu Yudha; Mahayani, Ni Putu Rika
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 11 No. 2 (2025): October 2025
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24428744.2025.v11.i2.17657

Abstract

Background: Peptic ulcer disease (PUD) was a persistent gastrointestinal disorder characterized by mucosal damage in the stomach or duodenum. Cyclea barbata leaves had been reported to contain bioactive compounds with gastroprotective potential. Objectives: This study investigated the protective effects of 70% ethanolic extract of C. barbata leaves on ethanol-induced gastric ulcers in rats. Methods: Thirty male rats were randomly allocated into six groups. The groups consisted of a normal control, a negative control (Na CMC 0.5%), a positive control (ranitidine 13.5 mg/kg BW), and three treatment groups that received C. barbata ethanolic extract at doses of 100, 200, and 400 mg/kg BW. Treatments were administered orally for 14 consecutive days, and the animals were sacrificed on day 15. Ulcer count, ulcer diameter, ulcer index, and gastric pH were assessed. Results: The negative control group exhibited an average of 6.4 ulcers. Administration of ranitidine and all tested concentrations of C. barbata extract significantly reduced the number of ulcers compared to the negative control. The most pronounced effect was observed at 400 mg/kg BW, yielding an average of 1 ulcer and a significantly lower ulcer index (p < 0.05). In contrast, gastric pH values showed no significant differences among the treatment and negative control groups (p > 0.05). Conclusions: The findings indicated that the ethanolic extract of C. barbata leaves exerted gastroprotective activity, with the 400 mg/kg BW dose demonstrating the strongest ulcer-preventive effect.
Co-Authors I Gede Agus Sindhu A Indraswari, Putu Ika Indah Mahayani, Ni Putu Rika Ni Putu Ayu Desi Astini Putu Yudha Ugrasena Ugrasena, Putu Yudha