Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal Majalah Obat Tradisional Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan
Daud, Intan
Unknown Affiliation
Health Professions Medicine & Pharmacology Public Health

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
MEKANISME KO-KEMOTERAPI EKSTRAK ROSELLA (Hibiscus sabdariffa. L) DENGAN DOXORUBICIN PADA SEL KANKER SERVIKS (HeLa) SECARA IN VITRO Daud, Intan; Hayu Nurani, Laela; Bachri, Moch. Saiful; Ariani Edityaningrum, Citra; Ma’ruf, Muhammad
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 9 No 2 (2024): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jiis.v9i2.2158

Abstract

Cervical cancer caused by exposure to Human Papilloma Virus (HPV) through sexual intercourse can be treated with Doxorubicin. Doxorubicin shows side effects so it needs to be combined with co-chemotherapy. Rosella (Hibiscus sabdariffa L.) The main content of anthocyanins is cytotoxic to HeLa cells.  The purpose of this study was to find co-chemotherapy combination of Doxorubicin and Rosella extract with apoptosis mechanism and Rosella antiproliferation.  The research method starts from the maceration process of Rosella petals using 96% ethanol. Cytotoxic test method, antiproliferation test, apoptosis, and combination test with MTT method using HeLa cells. The result of cytotoxic test showed IC50 of Rosella extract was 32.3 ± 2.15 µg/mL and IC50 of Doxorubicin was 2.68 ± 0.09 µg/mL. The antiproliferation test results in the control cell doubling time of 28.25 ± 0.21 hours while the concentration of Rosella extract at levels of 32.3; 16.15; and 8.08 µg/mL doubling time of 53.23 ± 0.20 hours, 39.02 ± 0.19 hours and 46.74 ± 0.23 hours so that it shows the inhibition of cell multiplication growth. The IC results show the combination of 4.04 µg/mL Rosella Flower Extract and 2.67 µg/mL Doxorubicin which is 0.0013 can be interpreted as a very strong synergistic effect (0.0013 ≤ 0.1). So it can be concluded the combination of ethanol extract Rosella flowers has antiproliferation properties through the mechanism of delaying the doubling time.
Cytotoxic Potential of Combination of Hibiscus sabdariffa L. Extract and Cisplatin against HeLa Cervical Cancer Cell Line: A Study of Antiproliferative Activity and Apoptosis Induction Nurani, Laela Hayu; Azzahra, Fara; Utami, Dwi; Edityaningrum, Citra Ariani; Guntarti, Any; Irham, Lalu Muhammad; Daud, Intan; Khairurrizki, Amanda; Putra, Ichsan Luqmana Indra; Ismiyati, Nur; Rofida, Siti; Sugiyanto, Sugiyanto
Majalah Obat Tradisional Vol 30, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.92440

Abstract

Cervical cancer is one of the most prevalent cancer types, making the development of effective anticancer agents critical. Cisplatin (CIS) is a drug that has been used to treat cancer, but it also affects normal cells. Hibiscus sabdariffa L. extract (HSE), which has the potential as an anticancer agent, can be developed as a co-chemotherapy with CIS. This study aimed to determine the potential of HSE as a co-chemotherapy with CIS against HeLa cervical cancer cells and determine specific and non-specific parameters of the studied extract. Hibiscus sabdariffa L. (HS) simplicia powder was macerated with 96% ethanol. An in vitro evaluation was carried out on antiproliferative activity by calculating the doubling time. Additionally, a selectivity test was conducted to calculate combination index (CI) values based on the microtetrazolium (MTT) method. Apoptosis mechanisms were explored based on immunocytochemical methods using p53, caspase, and Bax antibodies, followed by an observation of apoptotic induction using a flow cytometer. The HSE and CIS selectivity index values obtained for Vero cells and HeLa cells were 209 and 278, respectively. The antiproliferation test results showed that the combination of HSE and CIS could better extend the doubling time of cells compared to the negative control. This combination also demonstrated a strong synergistic effect, with a CI value of 0.001. The extract as a co-chemotherapy with CIS was capable of increasing the expression of p53, caspase-3, and Bax. The flow cytometry analysis results indicated that HSE could induce apoptosis. Based on the results on the IC50 and CI value of HSE, as well as on the doubling time and apoptosis induction of HSE-influenced HeLa cells, it is concluded that HSE has the potential as a co-chemotherapy against cervical cancer.
Co-Authors Any Guntarti Azzahra, Fara Citra Ariani Edityaningrum, Citra Ichsan Luqmana Indra Putra, Ichsan Luqmana Indra Khairurrizki, Amanda Laela Hayu Nurani Lalu Muhammad Irham Moch. Saiful Bachri Muhammad Ma'ruf Muhammad Yusron Maulana El-Yunusi Nur Ismiyati Siti Rofida Sugiyanto -