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All Journal Jaringan Laboratorium Medis Medica Arteriana (Med-Art)
Elen, Mircha Restiana
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health

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Appropriate Slice for Microscopic of Hepar and Kidney Mice Fixed With BNF 10% Armalina, Desy; Elen, Mircha Restiana; Labai, Nikita Ferren
Medica Arteriana (Med-Art) Vol 6, No 2 (2024): Desember 2024
Publisher : University of Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/medart.6.2.2024.43-49

Abstract

Background: Histological processing encompasses the steps of acquiring fresh tissue, preserving it through fixation to maintain its natural appearance, slicing it into thin sections, placing the sections on glass slides, and subsequently staining them. This enables the observation of various histological components within the tissue using a microscope. The objective of this study was to ascertain the microscopic characteristics of heparin preparations and the kidneys of mice (Mus musculus) through fixation using a formalin buffer solution.Method: This kind of research encompasses experimental research. The study employed a purposive sample methodology in its research design. This study concludes that preparations containing images provide a representative depic-tion of the cell nucleus, cytoplasm, and exhibit consistent coloration with different cutting thicknesses. The standard thickness for cutting the mice hepatic tissue using a microtome is 5 μm.Result: This thickness yields good microscopic results overall, with 25 field views from 5 preparations. The percentage of high-quality microscopic images obtained at this thickness is 100%. The statistical analysis revealed significant differences (p<0.05) between the 2 μm and 5 μm groups, as well as between the 5 μm and 8 μm groups. There was no statistically significant difference (p>0.05) observed between the 2 μm and 8 μm groups in terms of many aspects including structural, nucleolus, cytoplasm, color uniformity, and total structure scores.Conclusion: The optimum thickness in this research was 5 μm. Additional investigation is required to examine the microscopic characteristics of tissue samples by varying the duration of staining on the samples.
Profil Kualitas Mikroskopis Sediaan Hepar Mencit (Mus musculus) dengan Pemotongan Ketebalan 2µm, 5µm dan 8µm Elen, Mircha Restiana
Jaringan Laboratorium Medis Vol. 4 No. 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8483

Abstract

The cutting stage is the network manufacturing stage which needs to be considered the thickness scale. The standard scale that is commonly used is 2-7 µm, while the thickness of 5 µm which is used as a standard for cutting the thickness of the liver tissue shows that the cell is good for cutting liver tissue in mice (Mus musculus). Mice have many advantages, while the liver organ is one of the organs that are often used in research. The aim of this study was to describe the microscopic quality of liver preparations of mice (Mus musculus) by cutting tissue thickness of 2 µm, 5 µm and 8 µm. This type of research includes experimental research with descriptive research criteria. The study design used a purposive sampling study approach. The results of the quality of microscopic images of mice tissue (Mus musculus) at a thickness of 2 µm there were 12% not good, 20% not good and 68% good. The thickness of 5 µm is 100% good, while the thickness of 8 µm is 8%, the preparation is not good,  36% is not good, and 56% is good. The conclusions of this study were microscopic tissue mice (Mus musculus) preparations with good quality 68%, 8 µm 56%, and 100% at a thickness of 5 µm.
Co-Authors Desy Armalina Labai, Nikita Ferren