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PERBANDINGAN PEMERIKSAAN TINJA ANTARA METODE FLOTASI DAN METODE RITCHIE DALAM MENDETEKSI SOIL TRANSMITTED HELMINTH Wasilah, Nayla; Sulaeman; Sundara Mulia, Yuliansyah; Firman Solihat, Mohamad
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Microscopic stool examination has two examinations, namely qualitative and quantitative examination. Methods that can be done for qualitative fecal examination are direct slide method, anal swab method, flotation method, tape method, and sedimentation method. While in quantitative examination, namely Stoll, Ritchie, and Kato Katz. This study aims to determine the difference in the identification results of the number of eggs of each species found in the Flotation Method and the Ritchie Method. This research was conducted at the Parasitology Laboratory of the Polytechnic of the Ministry of Health Bandung in March-April 2024. The population in this study was obtained from feces suspected of Soil Transmitted Helminth. The number of samples used in this study were 30 samples using purposive sampling method, namely sampling techniques with certain criteria. Data analysis used the Paired Sample T Test statistical test by calculating the sensitivity and specificity values. The results showed that the number of Soil Transmitted Helminth eggs was found more in the ritchie method, namely in Ascaris lumbricoides 350 (84.95%) and Trichuris trichiura 62 (15.05%), while in the flotation method in Ascaris lumbricoides 195 (85.15%) and Trichuris trichiura 34 (14.85%). Flotation method and Ritchie method have the same sensitivity and specificity values, namely 100% sensitivity and 0% specificity. The results of the Paired Sample T Test have a significant value of 0.001 (p < 0.05) indicating that there is a difference in the results of STH examination with flotation and ritchie methods.
PERBANDINGAN PEMERIKSAAN JUMLAH TROMBOSIT METODE MANUAL (AMONIUM OKSALAT) DAN METODE PENCITRAAN DIGITAL (IMAGEJ) PADA KAMAR HITUNG IMPROVED NEUBAUER Gusmana, Riki; Firman Solihat, Mohamad; Nurhayati, Betty; Durachim, Adang; Kristian, Erick
Jurnal Kesehatan Siliwangi Vol. 6 No. 2 (2025): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

ABSTRACT Platelet count examination is a crucial parameter in evaluating hemostatic function, especially in diagnosing diseases such as dengue fever. This study compares the manual method using Ammonium Oxalate reagent with the digital imaging method utilizing ImageJ software on the Improved Neubauer chamber. The research aims to evaluate the differences between these methods and compare them with the automated method (Hematology Analyzer) as the gold standard. The study employed an observational analytic design with a cross-sectional approach. Samples consisted of blood from patients undergoing complete hematology examinations at RSUD Bedas Kertasari, Bandung Regency. Results from the three methods were compared to determine accuracy, efficiency, and objectivity. The results of the study showed the quantitative distribution of platelet counts using automatic (Hematology Analyzer), manual (Ammonium oxalate) and digital imaging (ImageJ) tools obtained the average values ​​respectively: 302,400 cells/mm3, 297,500 cells/mm3, 305,000 cells/mm3. There was no statistically significant difference in the test of platelet count examination data pairs between the automatic method (Hematology Analyzer) and the manual method (Ammonium oxalate) and between the automatic method data pairs (Hematology Analyzer) and the digital imaging method (ImageJ). While in the test of platelet count examination data pairs in the Improved Neubauer counting chamber between the manual method (Ammonium oxalate) and the digital imaging method (ImageJ) there was a statistically significant difference, but clinically the difference was still within the permissible threshold.