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PERBANDINGAN PEMERIKSAAN TINJA ANTARA METODE FLOTASI DAN METODE RITCHIE DALAM MENDETEKSI SOIL TRANSMITTED HELMINTH Wasilah, Nayla; Sulaeman; Sundara Mulia, Yuliansyah; Firman Solihat, Mohamad
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Microscopic stool examination has two examinations, namely qualitative and quantitative examination. Methods that can be done for qualitative fecal examination are direct slide method, anal swab method, flotation method, tape method, and sedimentation method. While in quantitative examination, namely Stoll, Ritchie, and Kato Katz. This study aims to determine the difference in the identification results of the number of eggs of each species found in the Flotation Method and the Ritchie Method. This research was conducted at the Parasitology Laboratory of the Polytechnic of the Ministry of Health Bandung in March-April 2024. The population in this study was obtained from feces suspected of Soil Transmitted Helminth. The number of samples used in this study were 30 samples using purposive sampling method, namely sampling techniques with certain criteria. Data analysis used the Paired Sample T Test statistical test by calculating the sensitivity and specificity values. The results showed that the number of Soil Transmitted Helminth eggs was found more in the ritchie method, namely in Ascaris lumbricoides 350 (84.95%) and Trichuris trichiura 62 (15.05%), while in the flotation method in Ascaris lumbricoides 195 (85.15%) and Trichuris trichiura 34 (14.85%). Flotation method and Ritchie method have the same sensitivity and specificity values, namely 100% sensitivity and 0% specificity. The results of the Paired Sample T Test have a significant value of 0.001 (p < 0.05) indicating that there is a difference in the results of STH examination with flotation and ritchie methods.
PERBANDINGAN LAMA WAKTU PENUNDAAN SPESIMEN FESES TERHADAP PEMERIKSAAN SOIL TRANSMITTED HELMINTH DENGAN METODE KATO−KATZ Jenita Nurrafikasari, Putri; Sulaeman; Sundara Mulia, Yuliansyah; Kurniawan, Entuy
Jurnal Kesehatan Siliwangi Vol. 5 No. 2 (2024): JURNAL KESEHATAN SILIWANGI
Publisher : Politeknik Kesehatan Kemenkes Bandung

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Abstract

Soil transmitted helminth (STH) is a parasitic worm whose spread requires soil media. The spesieses of STH that infect humans are Ascaris lumbricoides, Trichuris trichiura, Necator americanus, and Ancylostoma duodenale. The Kato-katz method is easy to perform but requires accuracy in counting the number of worm eggs. The number of worm eggs found can be influenced by the length of time the sample is delayed. This research aims to determine the difference in the number of STH eggs based on the length of time delayed fresh feces samples in less than 3 hours and delayed feces samples less than 72 hours using the Kato-katz method. The research was conducted with feces samples from Padjadjaran University which indicated the presence of STH eggs. The results found in the delayed feces samples < 72 hours eggs are less than in fresh feces samples in < 3 hours. The number of Ascaris lumbricoides eggs in fresh samples in < 3 hours was 4,950 EPG while in samples delayed < 72 hours was 3,495 EPG. The number of Trichuris trichiura eggs in fresh samples in < 3 hours was 510 EPG while in samples delayed < 72 hours was 450 EPG. Based on Twoway Anova test obtained sig value. < 0.05 from the time delay to the results of the eggs found, namely 0.037. So, it can be interpreted that there is an influence in the length of time delayed examination of feces samples on the number of Soil transmitted helminth eggs found.