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All Journal Indonesia Natural Research Pharmaceutical Journal (INRPJ) Anakes: Jurnal Ilmiah Analis Kesehatan
Wulandari, Destiana
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

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Identifikasi Jamur Penyebab Onikomikosis Pada Kuku Jari Kaki Petani Sawah Di Desa Rajeg Kabupaten Tangerang Mulyati; Rohadatul Aisy, Dzia Ulhaq; Wulandari, Destiana
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.1995

Abstract

Fungi are eukaryotic microorganisms that can grow in damp places. Onychomycosis is a fungal infection of the nails, which causes nail damage affecting approximately 10% of the world's population. Rice farming is a job that has a high risk of onychomycosis due to high activity in a humid environment. The aim of this research was to determine the description of the fungus that causes onychomycosis in the toenails of rice farmers in Rajeg Village, Tangerang Regency.This research uses a descriptive observation method with data analysis presented in the form of univariate and bivariate tables. The research was carried out in May-June 2023, with 34 samples taken using the technique random sampling. The research results showed that the most positive results for onychomycosis infection occurred in rice farmers with female gender at 55.9% and the productive age group 26-65 years at 64.7%. Apart from that, onychomycosis infections often occur in farmers who have worked >5 years, amounting to 82.3%, with the habit of not using footwear when working at 91.2% and farmers who do not wash their toenails with soap at 64.7%. Based on research, it is known that the frequency distribution of the fungus that causes onychomycosis in rice farmers by direct examination was found to be 41.2% and by culture examination was found to be 91.2% with the largest species found, namelyAspergillus sp 46.7%. Based on the research results, it can be concluded that length of work, habit of using footwear, and habit of washing toenails with soap are several factors that influence the occurrence of onychomycosis infection in farmers.   Keywords           : Fungi, Onychomycosis, Rice Field Farmers
Identifikasi Jamur Penyebab Onikomikosis Pada Kuku Jari Kaki Petani Sawah Di Desa Rajeg Kabupaten Tangerang Mulyati; Rohadatul Aisy, Dzia Ulhaq; Wulandari, Destiana
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.1995

Abstract

Fungi are eukaryotic microorganisms that can grow in damp places. Onychomycosis is a fungal infection of the nails, which causes nail damage affecting approximately 10% of the world's population. Rice farming is a job that has a high risk of onychomycosis due to high activity in a humid environment. The aim of this research was to determine the description of the fungus that causes onychomycosis in the toenails of rice farmers in Rajeg Village, Tangerang Regency.This research uses a descriptive observation method with data analysis presented in the form of univariate and bivariate tables. The research was carried out in May-June 2023, with 34 samples taken using the technique random sampling. The research results showed that the most positive results for onychomycosis infection occurred in rice farmers with female gender at 55.9% and the productive age group 26-65 years at 64.7%. Apart from that, onychomycosis infections often occur in farmers who have worked >5 years, amounting to 82.3%, with the habit of not using footwear when working at 91.2% and farmers who do not wash their toenails with soap at 64.7%. Based on research, it is known that the frequency distribution of the fungus that causes onychomycosis in rice farmers by direct examination was found to be 41.2% and by culture examination was found to be 91.2% with the largest species found, namelyAspergillus sp 46.7%. Based on the research results, it can be concluded that length of work, habit of using footwear, and habit of washing toenails with soap are several factors that influence the occurrence of onychomycosis infection in farmers.   Keywords           : Fungi, Onychomycosis, Rice Field Farmers
REVIEW ARTICLE: VALIDATION OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) METHODS IN THE ANALYSIS OF VOLATILE COMPOUNDS IN NATURAL MATERIALS Fauzan, Ki Agus Rifki; Wea Bii, Maria Febriani; Salsabila, Atikah; Rahma, Siti; Wulandari, Destiana; Yanto, Ari
INDONESIA NATURAL RESEARCH PHARMACEUTICAL JOURNAL Vol 10, No 2 (2025)
Publisher : Fakultas Farmasi Universitas 17 Agustus 1945 Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52447/inrpj.v10i2.9195

Abstract

Natural ingredients from plants are a major source of volatile compounds with biological activities such as antimicrobial, antioxidant, anti-inflammatory, and antifungal, particularly in essential oils. Testing of these compounds relies on reliable techniques such as gas chromatography-mass spectrometry (GC-MS), which separates and identifies components through retention time and mass spectra, with validation ensuring linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ). This review article assesses the use and validation of GC-MS methods for the analysis of volatile compounds from natural ingredients, based on national and international literature over the past five years. The review approach included a structured search of databases such as PubMed, ScienceDirect, Google Scholar, and SINTA journals, with a focus on validation parameters and GC-MS instrument settings. The findings show that GC-MS stably detects key compounds such as monoterpenes and sesquiterpenes with 97-99% accuracy, and validates them according to AOAC standards (R² > 0.99; RSD < 8%; recovery 80-100%), where different extraction techniques affect their biological content and effectiveness. GC-MS validation is important to encourage innovation of environmentally friendly natural products in the pharmaceutical, food, and cosmetic sectors as a substitute for synthetic preservatives.
Co-Authors Fauzan, Ki Agus Rifki Mulyati Rohadatul Aisy, Dzia Ulhaq Salsabila, Atikah Siti Rahma Wea Bii, Maria Febriani Yanto, Ari