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All Journal Science and Technology Indonesia Journal of Pharmaceutical and Sciences.
Ainaputri, Aliza Salsabila
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health

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Development of a Gelatin-Based Genomic Reference Material for Halal Authentication Using Real-Time PCR Rahma, Anisa Aula; Meilani, Nanda Diva; Sulistiawati; Ainaputri, Aliza Salsabila; Damara, Dandy Satria; Malau, Jekmal
Science and Technology Indonesia Vol. 10 No. 1 (2025): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.1.27-42

Abstract

Indonesia, home to over 270 million people, has the largest Muslim population globally, with approximately 87.18% adhering to Islam, driving significant demand for halal products, particularly in the food and pharmaceutical sectors. Gelatin, commonly used in medicinal capsules, often originates from porcine sources, necessitating precise halal authentication methods. This study presents the development of a novel genomic DNA-based Reference Material (RM) for gelatin, specifically for porcine DNA detection, employing Real-Time Polymerase Chain Reaction (qPCR) techniques. The methodology encompassed in-silico primer design, sample extraction optimization, DNA quality and quantity analysis, linearity assessment, limit of detection (LoD) and quantification (LoQ) determination, and RM characterization. Results indicated that the designed primers could reliably and efficiently detect porcine DNA, with optimal annealing at 58°C and primer concentration at 500 nM, achieving a PCR efficiency of 96.74%. The LoD and LoQ for pork meat samples were determined to be 0.02 pg/uL and 0.004 pg/uL, respectively, while the LoD for porcine gelatin was 0.27 ng/uL. The RMs exhibited robust homogeneity (Sig. 0.052), significant intergroup differences (Sig. 0.000), and low variation (CV 0.96%). Short-term storage at -80°C and -20°C preserved Ct value stability and consistency. Conclusively, this study successfully developed a novel gelatin-based genomic DNA RM for halal authentication, offering a scientifically validated tool that strengthens the halal assurance system, addressing Indonesian consumers’ demand for porcine-free products. These findings hold substantial implications for regulatory authorities, especially in Indonesia, and could inform the development of standardized qPCR RMs for porcine DNA detection in halal compliance testing.
Menelusuri Perkembangan, Kondisi Terkini, dan Prospek Masa Depan Pengujian DNA dalam Aplikasi Forensik Manusia dan Non-Manusia: Tinjauan Naratif Siboro, Dewi Pratiwi Purba; Zahra, Aliya Azkia; Kasasiah, Ahsanal; Malau, Jekmal; Aprillia, Cantika; Ainaputri, Aliza Salsabila; Nugraha, Afif Tri; Aprianti, Endeh
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 1 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i1.1389

Abstract

DNA typing has become a cornerstone of modern forensic science, profoundly influencing criminal investigations, forensic human identification, and non-human forensic applications. Since its introduction in the mid-1980s, forensic DNA analysis has evolved from restriction fragment length polymorphism-based methods to polymerase chain reaction-based short tandem repeat profiling, and more recently to sequence-based approaches enabled by massively parallel sequencing, resulting in substantial improvements in analytical sensitivity, robustness, and discriminatory power. This narrative review aims to trace the historical development, examine the current state, and explore future directions of DNA typing in both human and non-human forensic contexts, with particular emphasis on empirical case studies from Asia. A narrative review methodology was employed through a comprehensive analysis of peer-reviewed literature published between 2015 and 2026, sourced from major scientific databases including Google Scholar, PubMed, and ScienceDirect, with studies selected based on forensic relevance, methodological rigor, and regional significance. The review highlights the extensive application of DNA typing in routine criminal casework, disaster victim identification, missing persons investigations, wildlife forensic genetics, food fraud detection, and biosecurity, and documents emerging technologies such as portable DNA systems and CRISPR-based detection. Despite these advances, significant challenges remain related to data interpretation, validation requirements, contamination control, ethical and legal governance, and uneven forensic capacity across regions. Overall, this review underscores the continuing evolution of forensic DNA typing and emphasizes the importance of standardized protocols, interdisciplinary collaboration, and region-specific validation to ensure the reliable and responsible application of DNA evidence in modern forensic science.
Genetika dan Farmakogenomika untuk Kinerja Atletik: Tren Terkini dan Arah Masa Depan di ASEAN Nugraha, Afif Tri; Malau , Jekmal; Kasasiah , Ahsanal; Aprillia, Cantika; Ainaputri, Aliza Salsabila; Siboro , Dewi Pratiwi Purba
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 1 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i1.1375

Abstract

The integration of sport genomics and pharmacogenomics has become a central pillar of precision sports medicine, enabling individualized training, nutrition, and medication/supplement strategies for athletes. This structured narrative review synthesizes literature published between 2015 and 2025 from PubMed, ScienceDirect, and Google Scholar, with particular emphasis on implementation relevance in ASEAN, especially Indonesia. Findings are organized into three major themes: (1) genetic contributions to strength and endurance phenotypes, body composition, recovery, and injury susceptibility; (2) pharmacogenomic implications for drug selection, dose optimization, therapeutic safety, and potential exercise with drug interactions (e.g., NSAIDs, inhaled bronchodilators, caffeine, and creatine); and (3) ethical, privacy, equity, and gene-doping considerations. Athletic performance is inherently polygenic; variants in genes such as ACTN3, ACE, PPARGC1A, PPARA, and VEGF-A contribute to the power–endurance spectrum and vascular–metabolic adaptation, while pharmacogenes including CYP2C9, CYP1A2, ADORA2A, and ADRB2 may modulate therapeutic efficacy and adverse-effect risk. Moreover, physical activity itself may influence drug metabolism and pharmacodynamic responses, underscoring the limitations of one-size-fits-all protocols. Overall, genetic information should be positioned as a complementary decision-support layer rather than a deterministic predictor in sports medicine practice. Strengthening implementation in ASEAN requires larger, well-characterized athlete cohorts, standardized marker panels, longitudinal data integration, and robust ethical–regulatory frameworks to ensure safe and evidence-based genomic application.
Co-Authors Ahsanal Kasasiah Aprianti, Endeh Aprillia, Cantika Damara, Dandy Satria Jekmal Malau Kasasiah , Ahsanal Malau , Jekmal Meilani, Nanda Diva Nugraha, Afif Tri Rahma, Anisa Aula Siboro , Dewi Pratiwi Purba Siboro, Dewi Pratiwi Purba Sulistiawati Zahra, Aliya Azkia