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All Journal Universitas Muhammadiyah Yogyakarta Undergraduate Conference Proceeding Prosiding Seminar Nasional Program Pengabdian Masyarakat
Putri, Cut Laras Dwi Hendia
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Optimization of Temperature to Bacteriocin by Lactobacillus acidophilus Against Methicillin-Resistant Staphylococcus aureus Putri, Cut Laras Dwi Hendia; Suryani, Lilis
Proceedings of Universitas Muhammadiyah Yogyakarta Graduate Conference Vol. 4 No. 2 (2025): Renewable Energy, Environment, and Technology for Sustainable Transformation
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/umygrace.v4i2.664

Abstract

Introduction: Methicilin resistant Staphylococcus aureus (MRSA) is a pathogenic bacterium that has evolved resistance to antibiotics. MRSA is resistant to the antibiotics methisillin, cephalosporin, and betalaktam. MRSA causes many health problems ranging from lung damage to septicemia. The prevalence of MRSA infections in the world reached 14.69%. Lactobacillus acidophilus produces an antimicrobial substance bacteriosine. These bacteriosins have bactericidal and bacteriostatic activity. Purpose: The study aims to recognize the influence of temperature on the activity of bacteriosins produced by L.acidophilus against MRSA. Methode: This research used a quasi-experimental method. L.acidophilus reproduced on liquid MRS media for 48 hours. Bacteriosine is obtained by centrifying a MRS broth containing bacteria at a speed of 6000 rpm for 40 minutes. The fluid was tested for its antibacterial effects on MRSA bacteria using Kirby Bauer's method. Bacteriosine is given at different temperatures that is; 40, 60, 80, dan 100 oC. The diameter of the formed barrier zone is measured in millimeters. Data analysis with ANOVA method. Finding: Bacteriosine activity produced by Lactobacilus acidophilus against MRSA shows an average inhibition zone diameter of 5.25 mm. The optimum broadest spectrum of antibacterial activity at temperature 60 oC. Conclusion: This research shows that temperature does not affect the antibacterial activity of bacteriosin produced by L.acidophilus against MRSA. Originality/Implication: The results of this study prove that temperature treatment with various variations does not affect the ability of the bacteriosine produced by L.acidophilus to inhibit/kill MRSA.
Peningkatan Kesadaran Masyarakat tentang Hipertensi melalui Edukasi dan Skrining di Dusun Cabean Ashidiqi, Mahardhika Zulfa; Satritama, Ajar; Anggun Prima Jannata; Ananta, Ardian Surya; Putri, Cut Laras Dwi Hendia; Qur'aini, Farra Adina; Mukti, Laksono Hardi; Lestari, Putri Hayu; Fazaril A, Rasyad; Rosandia, Resita Nike; Meida, Nur Shani
Prosiding Seminar Nasional Program Pengabdian Masyarakat Vol. 8 No. 1 (2025): Rekonstruksi Pendidikan di Indonesia
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/ppm.81.1290

Abstract

Hypertension is one of the non-communicable diseases that contribute to high mortality and health complications, especially in the elderly. Based on observations and discussions with community leaders and posyandu cadres, it was found that the incidence of hypertension in Cabean Hamlet is quite high. Therefore, this KKN program emphasizes hypertension counseling, elderly gymnastics, family care for people with hypertension, and balanced nutrition education as the main strategies in preventing and treating hypertension. The purpose of the Community Service Program (KKN) in Cabean Hamlet, Bumirejo, Lendah, Kulon Progo is to increase public awareness about hypertension so that it can reduce mortality and complications due to hypertension. The methods used in this program include educational approaches and hypertension screening. The educative approach was conducted through lectures and interactive discussions about hypertension and a healthy diet to prevent hypertension. Hypertension screening was carried out through blood pressure checks (which were carried out before and after the elderly gymnastics) and measurement of body weight and height.The results of the activity showed that there was an increase in public awareness about hypertension. The results of blood pressure checks showed a decrease in blood pressure in most participants after doing elderly exercises, with an increase in the percentage of blood pressure becoming normal (from 38.9% to 58.3%). In addition, in the family care program, two elderly people who were monitored showed different results, one had improved blood pressure after routinely taking antihypertensive drugs, while the other continued to have high blood pressure due to non-compliance with treatment. The results showed that there are still some residents with non-ideal nutritional status, both obese and malnourished, who are at risk of hypertension. In conclusion, the service-learning program succeeded in raising community awareness about hypertension and had a positive impact on lowering the blood pressure of the elderly through comprehensive interventions. The success of this program shows the importance of the role of family and community in supporting sustainable public health.
Co-Authors Ananta, Ardian Surya Anggun Prima Jannata Ashidiqi, Mahardhika Zulfa Fazaril A, Rasyad Lestari, Putri Hayu Lilis Suryani Mukti, Laksono Hardi Nur Shani Meida Qur'aini, Farra Adina Rosandia, Resita Nike Satritama, Ajar