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All Journal Makara Journal of Science Biodiversitas Journal of Biological Diversity
MAZYTHA KINANTI RACHMANIA
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Indonesia, Kampus UI Depok, Depok 16424, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Activities of Cellulolytic, Amylolytic, and Secondary Metabolites from Thermophilic Actinomycetes Collected from the Heated Soil around Cisolok Geyser Sari, Dhian Chitra Ayu Fitria; Ningsih, Fitria; Rachmania, Mazytha Kinanti; Yokota, Akira; Sjamsuridzal, Wellyzar
Makara Journal of Science Vol. 28, No. 4
Publisher : UI Scholars Hub

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Abstract

This study investigated the antimicrobial activity, cellulolytic, and amylolytic abilities of eight thermophilic Actinomy-cetes isolated from the heated soil around the Cisolok geyser, West Java, Indonesia. Cellulolytic and amylolytic abili-ties were screened for on media by adding 1% (w/v) soluble starch and carboxymethylcellulose (CMC), respectively. Antimicrobial activity was screened for using the agar-plug diffusion method by cultivating strains on various growth media. Growth at various temperatures showed that six strains could grow up to 50 °C. Seven out of eight strains showed amylolytic ability at 40 °C and 45 °C, and only four strains did so at 50 °C. Screening for cellulolytic ability showed that seven strains could hydrolyze CMC at 40 °C, whereas seven and five strains could hydrolyze at 45 °C and 50 °C, respectively. Antibacterial activity showed that strains UICC B-110 and UICC B-118 grown on various media could inhibit the growth of Gram-positive bacteria. The results of the antifungal activity test showed that strain UICC B-118, incubated for seven and 14 days, could inhibit the growth of Candida albicans. Based on 16S rRNA gene se-quence similarity and phylogenetic analyses, strains UICC B-110 and UICC B-118 were identified as Streptomyces glaucescens and Microbispora rosea subsp. rosea, respectively. These results show that thermophilic Actinomycetes from the soil around the Cisolok geyser have the potential as a source of thermostable enzymes and new bioactive compounds with antimicrobial activity.
Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia WINDA AYU SYAFITRI; FITRIA NINGSIH; PUTRI PRATIWI SETYANINGSIH; MAZYTHA KINANTI RACHMANIA; DHIAN CHITRA AYU FITRIA SARI; SHUHEI YABE; AKIRA YOKOTA; ARIYANTI OETARI; WELLYZAR SJAMSURIDZAL
Biodiversitas Journal of Biological Diversity Vol. 20 No. 7 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d200720

Abstract

Abstract. Syafitri WA, Ningsih F, Setyaningsih PP, Rachmania MK, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridzal W. 2019. Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a geothermal area in West Java, Indonesia. Biodiversitas 20: 1929-1938. In this study, we describe the screening for amylolytic activity of 17 thermophilic Actinobacteria isolates obtained from the soil of Cisolok geysers, a geothermal area in West Java, Indonesia. All isolates were screened for amylolytic activity by the starch-agar plate method at various temperatures. The results showed that all of isolates were able to grow at 45oC. The growth abilities of the isolates grown in ISP 1 medium varied at temperatures from 45 to 60oC. Fifteen of the 17 isolates showed amylolytic activity at 45oC, 13 showed such activity at 50oC, and four showed activity at 55oC. Only three isolates, designated SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4, showed growth and amylolytic activity at 60oC. These three isolates were selected for molecular identification. The nearly full-length of 16S rRNA gene sequences data showed that these three isolates have a similarity of 99.93-100% with Actinomadura keratinilytica WCC-2265T and of 98.74-98.91% with A. miaoliensis BC 44T-5T. Phylogenetic tree shows that all three isolates are clustered together in a monophyletic group with the type strain of A. keratinilytica WCC-2265T as their most closely related species, with 100% bootstrap support. Based on sequencing of the 16S rRNA gene, phylogenetic comparison, and phenotypic characterization, the three isolates were identified as A. keratinilytica.
Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia PUTRI PRATIWI SETYANINGSIH; FITRIA NINGSIH; MAZYTHA KINANTI RACHMANIA; WINDA AYU SYAFITRI; DHIAN CHITRA AYU FITRIA SARI; SHUHEI YABE; AKIRA YOKOTA; ARIYANTI OETARI; Wellyzar Sjamsuridzal
Biodiversitas Journal of Biological Diversity Vol. 20 No. 11 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201105

Abstract

Abstract. Setyaningsih PP, Ningsih F, Rachmania MK, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridzal W. 2019. Cellulolytic enzyme-producing thermophilic Actinobacteria isolated from the soil of Cisolok Geysers, West Java, Indonesia. Biodiversitas 20: 3134-3141. This study investigated 17 thermophilic Actinobacteria isolated from the soil of geysers in the Cisolok geothermal area, West Java, as potential producers of cellulase. Screening for cellulase was performed on minimal (Mm) agar medium with and without the addition of 1% (w/v) carboxymethylcellulose (CMC) and microcrystalline cellulose (MCC), then incubated at 45, 50, 55 and 60°C for up to 7 days. Formation of clear zones around colonies indicated cellulose hydrolysis. The results showed that 15, 14, 4, and 3 isolates showed cellulolytic activity on CMC agar medium at 45, 50, 55, and 60°C, respectively, after 7 days of incubation. Three potential isolates showed cellulolytic activity on MCC agar medium after being incubated for 7 days at 45°C. Molecular identification based on the 16S rRNA gene was performed for three isolates with positive cellulolytic activity at 60°C. The results showed that the three isolates are closely related to Actinomadura keratinilytica WCC-2265T with 99.93-100% sequence similarities. A phylogenetic tree based on 16S rRNA gene sequences confirmed that the three isolates were clustered together with Actinomadura keratinilytica WCC-2265T with 100% bootstrap value. The tree also showed that cellulase producers and non-cellulase producers in Thermomonosporaceae are grouped into different clades.
Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area MAZYTHA KINANTI RACHMANIA; FITRIA NINGSIH; PUTRI PRATIWI SETYANINGSIH; WINDA AYU SYAFITRI; DHIAN CHITRA AYU FITRIA SARI; SHUHEI YABE; AKIRA YOKOTA; ARIYANTI OETARI; Wellyzar Sjamsuridzal
Biodiversitas Journal of Biological Diversity Vol. 21 No. 1 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210119

Abstract

Abstract. Rachmania MK, Ningsih F, Setyaningsih PP, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridjal W. 2020. Xylan-degrading ability of thermophilic Actinobacteria isolated from soil in a geothermal area. Biodiversitas 21: 144-154. This study was conducted to obtain the potential isolates of xylan-degrading thermophilic Actinobacteria. Seventeen isolates were obtained from the soil samples collected from Cisolok geysers in West Java, Indonesia. These isolates were screened for their xylan-degrading ability on minimal medium with the addition of 0.5% xylan as substrate, and were incubated at various temperatures for 7 days. A total of 15, 14, 4, and 3 isolates showed a xylan-degrading ability at 45°C, 50°C, 55°C, and 60°C, respectively. The three isolates (SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4) that showed xylan-degrading ability at 60°C on 0.5% xylan were further tested by using minimal medium with the addition of 0.1% RBB-xylan as a substrate. The results showed that these isolates were also able to utilized RBB-xylan at 45 to 60°C. The xylanolytic activity of the crude enzymes from the three isolates on minimal medium containing 0.5% xylan or 0.1% RBB-xylan as substrates, indicated that these isolates were able to produce extracellular xylanase. This study revealed that the soil of Cisolok geysers served as an ideal source for finding the thermophilic Actinobacteria which produced xylanase at high temperatures.
Identification and screening of enzymatic abilities of Ktedonobacteria from forest soil of Cisolok Geothermal Area, Indonesia MAZYTHA KINANTI RACHMANIA; FITRIA NINGSIH; DHIAN CHITRA AYU FITRIA SARI; YURIZA ESHANANDA; YASUTERU SAKAI; SHUHEI YABE; AKIRA YOKOTA; WELLYZAR SJAMSURIDZAL
Biodiversitas Journal of Biological Diversity Vol. 23 No. 9 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230935

Abstract

Abstract. Rachmania MK, Ningsih F, Sari DCAF, Eshananda Y, Sakai Y, Yabe S, Yokota A, Sjamsuridzal W. 2022. Identification and screening of enzymatic abilities of Ktedonobacteria from forest soil of Cisolok Geothermal Area, Indonesia. Biodiversitas 23: 4686-4695. This study aimed to provide information on culturable Ktedonobacteria from forest soil in the Cisolok geothermal area and their potential as enzyme producers. Twelve ktedonobacterial isolates were obtained from this study and identified based on full-sequence of the 16S rRNA gene. Seventeen isolates (including five isolates from previous studies) were used for enzymatic screening and phylogenetic analyses. Screening of amylolytic (0.5% soluble starch) and cellulolytic (0.5% carboxymethylcellulose) (CMC) activities from ktedonobacterial isolates was performed on a ten-fold diluted R2A agar medium, and were incubated at 30 °C for 21 days. The EzBioCloud search revealed that all isolates showed low sequence similarities with Dictyobacter aurantiacus S-27T (97.82 to 98.18%) as their closest related species. The phylogenetic tree showed that all isolates belong to the genus Dictyobacter (family Dictyobacteraceae of the class Ktedonobacteria). All isolates formed a monophyletic group and were placed as the sister clade to D. aurantiacus, as supported by a very strong bootstrap value (99%). Screening of amylolytic and cellulolytic abilities showed that most isolates (88.23%) were able to degrade both 0.5% starch and 0.5% CMC as substrates. This study revealed the presence of Ktedonobacteria with amylolytic and cellulolytic abilities in the forest soil of Cisolok geothermal area.
Co-Authors AKIRA YOKOTA AKIRA YOKOTA AKIRA YOKOTA ARIYANTI OETARI ARIYANTI OETARI ARIYANTI OETARI DHIAN CHITRA AYU FITRIA SARI DHIAN CHITRA AYU FITRIA SARI DHIAN CHITRA AYU FITRIA SARI DHIAN CHITRA AYU FITRIA SARI fitria ningsih Fitria Ningsih PUTRI PRATIWI SETYANINGSIH PUTRI PRATIWI SETYANINGSIH PUTRI PRATIWI SETYANINGSIH Sari, Dhian Chitra Ayu Fitria SHUHEI YABE SHUHEI YABE SHUHEI YABE WELLYZAR SJAMSURIDZAL WINDA AYU SYAFITRI WINDA AYU SYAFITRI WINDA AYU SYAFITRI YASUTERU SAKAI Yokota, Akira YURIZA ESHANANDA