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Winarto Haryadi
Department of Chemistry, Universitas Gadjah Mada, Yogyakarta-55281 (Indonesia)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics

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Profiling and Antibacterial Activity Assay of Secondary Metabolites from Streptomyces Isolated from Mangrove Sediment Sample Dwi Marwati Juli Siswanti; Kasta Gurning; Winarto Haryadi; Chairil Anwar; Respati Tri Swasono
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.236

Abstract

Bacterial infections pose significant threats to human health, particularly due to the rise of antibiotic-resistant strains. Identifying new sources of effective antibiotics is therefore crucial for combating these resistant pathogens. This study aims to isolate novel Streptomyces species and profile their secondary metabolites through extraction and high-resolution mass spectrometry (HRMS) analysis. Furthermore, the antibacterial activity of the extract containing the secondary metabolites was assessed through the in vitro agar well diffusion method and supported by the molecular docking, molecular dynamics simulations, and drug-likeness analysis. Sediment samples were collected from mangrove forests in Yogyakarta. The bacteria were then isolated, purified, and characterized using 16S rRNA sequencing. The isolates were then cultured to enrich the secondary metabolites, and their secondary metabolites were extracted using methanol and dichloromethane solvents in a 1:1 volume ratio. The results showed that the isolated bacteria of Streptomyces sp. were obtained with a 95.44% similarity rate, which produced several secondary metabolites. The in vitro antibacterial assay of the extract resulted in an inhibition zone of 14, 14, and 15 mm against Propionibacterium acnes, Staphylococcus aureus, and Escherichia coli, respectively. The molecular docking and molecular dynamic simulations for 100 ns revealed that compound SB236057A could inhibit the function of thymidylate kinase protein through a carbon-hydrogen bond with Glu37 residue. Furthermore, drug-likeness analysis showed that the secondary metabolites of Streptomyces sp. exhibited preferable drug-likeness and pharmacokinetic properties. This research focuses on the understanding of microbial biodiversity in mangrove sediments, particularly focusing on the genus Streptomyces and its potential to produce novel antibiotics.
Potential of Bioactive Compounds In Coleus amboinicus, Lour., Leaves Against Breast Cancer By Assessment Using A Network Pharmacology Approach and Cytotoxic Test Winarto Haryadi; Kasta Gurning; Jajah Fachiroh; Endang Astuti
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.246

Abstract

Breast cancer is a disease that significantly contributes to global women death. The study aims to conduct in vitro activity testing and assessment with a bioinformatics approach using a pharmacological network of bioactive compounds from bangun-bangun (Coleus amboinicus) leaves extract as a breast cancer drug. The methods used are extraction of bioactive compounds by maceration and partition, identification and analysis of bioactive compounds using the Liquid Chromatography High-Resolution Mass Spectrometry (LC-HRMS) instrument, cytotoxic testing of breast cancer cells (MCF-7) and normal cells (CV-1) with the MTT method, and assessment with a bioinformatics approach through a network pharmacology. The results of the cytotoxic test of ethyl acetate extract provided better activity with IC50 value of 102.30 and 457.09 µg/mL against MCF-7 cancer cells and CV-1 normal cells. The selectivity index value of 4.23 indicates the potential for further development in the treatment of breast cancer. The results of the analysis of chemical compound content show various types of potential bioactive compounds as breast cancer anticancer; assessment of the bioinformatics approach through networks pharmacology with pathways in cancer provides predictions of signal transducer and activator of transcription 3 (STAT3) protein as the main therapeutic mechanism target in breast cancer treatment. This study provides initial information for further research on testing and utilizing bioactive compounds from C. amboinicus leaves as an alternative treatment for breast cancer.
Co-Authors Chairil Anwar Dwi Marwati Juli Siswanti Endang Astuti Jajah Fachiroh Kasta Gurning Respati Tri Swasono