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Bintang Pamungkas, Puguh
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Aerospace Engineering Agriculture, Biological Sciences & Forestry Materials Science & Nanotechnology

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Identifikasi Bakteri Endofit Dalam Penerapan Eco-Enzyme Pada Budidaya Jahe Merah (Zingiber Officinale Var. Rubrum) Bintang Pamungkas, Puguh; Purwaningsih, Okti; A Hi Wahid, Rahmat
JURNAL ILMIAH AGRINECA Vol. 25 No. 1 (2025): JURNAL ILMIAH AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v25i1.4365

Abstract

During the COVID-19 pandemic, the use of red ginger as a herbal treatment increased significantly, establishing it as a highly popular herbal commodity. However, disruptions caused by plant pests have led to fluctuating demand. One major issue is bacterial wilt disease caused by Ralstonia solanacearum, which can result in yield losses of up to 90%. Biological control using endophytic bacteria has shown promise in managing bacterial wilt, particularly bacteria residing in the red ginger rhizosphere. This research aimed to explore and identify endophytic bacteria from the rhizosphere of red ginger plants. The study was conducted from September to October 2022 at the Greenhouse and Integrated Chemistry and Biology Laboratory, PGRI University Yogyakarta. Soil samples were collected from red ginger cultivation systems implementing eco-enzymes as part of a healthy farming approach. Four treatments were applied with three replications: no eco-enzyme, 0.1% eco-enzyme, 0.3% eco-enzyme, and 0.5% eco-enzyme. Each treatment involved three soil samplings, yielding a total of 36 soil samples for analysis. Exploration of the rhizosphere resulted in the isolation of 36 bacterial strains, of which 20 were identified as belonging to the genera Bacillus sp. and Pasteuria sp. Among these, 16 isolates were identified as Bacillus sp., including six from the no eco-enzyme treatment, one from the 0.3% eco-enzyme treatment, and nine from the 0.5% eco-enzyme treatment. Additionally, five isolates were identified as Pasteuria sp., with two from the 0.1% eco-enzyme treatment and three from the 0.3% eco-enzyme treatment.
Karakterisasi Jamur Patogen Penyebab Penyakit Pada Tanaman Srikaya (Annona Squamosa) di UD. Sabila Farm: Karakterisasi Jamur Patogen Penyebab Penyakit Pada Tanaman Srikaya (Annona Squamosa) di UD. Sabila Farm Purnomo, Azis; Pratama, Dion; Bintang Pamungkas, Puguh; Kandito, Argawi
JURNAL ILMIAH AGRINECA Vol 25 No 2 (2025): JURNAL ILMIAH AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v25i2.4998

Abstract

Soursop (Annona squamosa) is a horticultural crop with high economic value. However, this plant is susceptible to diseases caused by pathogenic fungal infections, which can reduce yield and fruit quality. This study aims to identify pathogenic fungi affecting soursop plants at UD. Sabila Farm and understand their role in disease development. The research was conducted at UD. Sabila Farm, located in Kapanewon Pakem, Sleman Regency, Special Region of Yogyakarta. Further laboratory analyses were carried out at the Biology Laboratory, Faculty of Agriculture, Universitas PGRI Yogyakarta. The study employed field exploration and laboratory identification using Potato Dextrose Agar (PDA), samples were collected from leaves, stems, and fruits showing signs of infection. The findings revealed several fungal pathogens contributing to plant diseases, such as Colletotrichum sp., Fusarium solani sp., and Curvularia sp.. Morphological characterization and pathogenicity analysis provide a foundation for developing effective disease management strategies. This research is expected to support farmers in maintaining plant health and improving soursop production sustainably.
Co-Authors A Hi Wahid, Rahmat Kandito, Argawi OKTI PURWANINGSIH Pratama, Dion Purnomo, Azis