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Review Artkel Efek Sinergis Ekstrak Nabati terhadap Pengendalian Bakteri Patogen pada Produk Pertanian dan Kesehatan Rezky Maulidiya; Ardi Mustakim
Mikroba : Jurnal Ilmu Tanaman, Sains Dan Teknologi Pertanian Vol. 2 No. 1 (2025): April : Mikroba : Jurnal Ilmu Tanaman, Sains Dan Teknologi Pertanian
Publisher : Asosiasi Riset Ilmu Tanaman Dan Hewan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/mikroba.v2i1.248

Abstract

Vegetable ingredients are natural sources of active compounds that have the potential to be used as control agents for pathogenic microorganisms in various fields, including health and agriculture. This study aims to evaluate the synergistic effect between aloe vera (Aloe vera L.) and bandotan (Ageratum conyzoides L.) extracts on inhibiting the growth of pathogenic bacteria in different media. Aloe vera extract is obtained using the infundation method, while bandotan extract is obtained using the maceration technique. The concentrations tested were 30%, 45%, and 60% for each extract, as well as a combination of both. Testing was carried out on two main applications: as an antiseptic hand sanitizer by testing germ colonies using Plate Count Agar (PCA), and as a bacteria control Erwinia carotovora, the cause of carrot tuber rot, using the Path Ways Analysis test. The results showed that the combination of aloe vera and bandotan extracts had more significant inhibitory effectiveness than single extracts in both applications. A combination concentration of 60% gave the best results with a reduction in bacterial colonies of up to 90%. The mechanism of action of active compounds such as flavonoids, phenolics and saponins in this vegetable extract plays an important role in damaging bacterial cell membranes and inhibiting the metabolism of microorganisms. This research confirms the potential of using local plant materials as an environmentally friendly and economical solution for controlling pathogenic bacteria in the health and agricultural sectors.
Pengaruh Ekstrak Orthosiphon Aristatus terhadap Pertumbuhan Mikroorganisme Uji dalam Praktikum Mikrobiologi Dasar Rezky Maulidiya; Ardi Mustakim
Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa Vol. 3 No. 4 (2025): Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa
Publisher : Asosiasi Riset Ilmu Matematika dan Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/algoritma.v3i4.643

Abstract

Orthosiphon aristatus is a medicinal plant that has the potential as a natural antibacterial agent. This study aims to evaluate the effect of cat's whiskers leaf extract on microbial growth using Nutrient Agar (NA) media with variations in extract concentrations of 100 gr, 50 gr, and 25 gr. Antibacterial activity tests were carried out using the well diffusion paper disk method, and observations of microbial growth inhibition zones were carried out after incubation for 24 hours at 37°C. The test results showed that there was inhibition of microbial growth that varied according to the concentration of the extract used; a concentration of 100 gr gave the largest inhibition zone compared to 50 gr and 25 gr. Data analysis showed that increasing extract concentration was directly proportional to increasing antibacterial effectiveness. These findings indicate that Orthosiphon aristatus leaf extract has the potential as a natural antibacterial that is effective in inhibiting microbial growth in NA media, so it has the potential to be developed as an active ingredient in pharmaceutical formulations.