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All Journal Airlangga Journal of Innovation Management
Haryo Triajie
University of Trunojoyo Madura
Economics, Econometrics & Finance Social Sciences

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Formulation of Indigenous Bacterial Consortium From Salt Wastewater (Bittern) in Degrading Plastic Abdus Salam Junaedi; Maftuh Ahnan; Putri Wahyuningtyas; Muhammad Zainuri; Haryo Triajie
Airlangga Journal of Innovation Management Vol. 7 No. 2 (2026): Airlangga Journal of Innovation Management
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ajim.v7i2.81616

Abstract

Plastic is classified as a synthetic polymer with a long and repetitive chain structure, making its decomposition process very slow. This study aims to measure the optical density (OD) value, determine the total plate count (TPC) value, and compare the plastic degradation activity between the indigenous bacterial formulation from salt washing wastewater and the control bacterial formulation. The optical density (OD) values of the indigenous bacteria from salt washing wastewater (bittern) in degrading plastic waste in Yeast Extract Broth (YEB) + 0.1% glucose medium by formulations F1, F2, F3, and F4 after 7 days of incubation were 1.595, 1.592, 1.488, and 1.645, respectively. The total plate count (TPC) values of the indigenous bacteria from salt washing wastewater (bittern) in degrading plastic waste in Yeast Extract Broth (YEB) + 0.1% glucose medium by formulations F1, F2, F3, and F4 after 7 days of incubation were 13.66 Log CFU/mL, 13.66 Log CFU/mL, 13.73 Log CFU/mL, and 13.62 Log CFU/mL, respectively. The percentage of plastic dry weight after 7 days of incubation in Yeast Extract Broth (YEB) + 0.1% glucose medium produced by indigenous bacteria from salt washing wastewater (bittern) F1, F2, F3, and F4 were 10.43%, 40%, 15.89%, and 15.68%, respectively. This study demonstrates the potential of indigenous bacterial formulations from salt washing wastewater (bittern) as effective plastic biodegradation agents, contributing positively to the development of environmental biotechnology, and serving as a reference for industries and policymakers in promoting sustainable plastic waste management.
Application of Indigenous Bacterial Formulation of Salt-Washing Wastewater (Bittern) in Degrading Plastic Waste Aliefia Miftakhul Ardani; Wahyu Dwi Saputri; Abdus Salam Junaedi; Haryo Triajie
Airlangga Journal of Innovation Management Vol. 5 No. 4 (2024): Airlangga Journal of Innovation Management
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ajim.v5i4.65189

Abstract

The accumulation of plastic waste can lead to environmental changes. One solution to reduce plastic waste is through a biodegradation process involving microorganisms or bacteria. One solution is biodegradation by using microorganisms or bacteria to break down plastic. Biodegradation is a solution to reduce plastic numbers with the help of microorganisms or bacteria. This study aims to measure the Optical Density (OD), Total Plate Count (TPC), and degradation effectiveness of indigenous bacteria from salt wash wastewater (bittern) in degrading plastic on Nutrient Broth (NB) media with 1% glucose. The research process included sterilization of tools and materials, sampling, media preparation, rejuvenation of bacterial cultures, inoculation, OD measurement, TPC calculation, and percentage of plastic weight loss. Data on OD and TPC were analyzed quantitatively with visual aids. The plastic degradation percentage was measured after 7 days of incubation. The percentage of plastic degradation was measured after 7 days of incubation. The results showed that the highest OD value was bacteria BB8 which amounted to 1.614. TPC for plastic degradation was highest in BB9 which amounted to 13.37 Log CFU/g. BB4 showed the highest percentage of plastic dry weight reduction 36.4% after 7 days. These findings highlight the potential of salt-washing wastewater bacteria for plastic waste degradation.
Co-Authors Abdus Salam Junaedi Aliefia Miftakhul Ardani Maftuh Ahnan Muhammad Zainuri Putri Wahyuningtyas Wahyu Dwi Saputri