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All Journal Jurnal Riset Ilmu Kesehatan Umum dan Farmasi
Dedi Nofiandi
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Health Professions Public Health

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Penetapan Kadar Beta Karoten pada Ekstrak Aseton Buah Blewah (Cucumis Melo L.) Berdasarkan Tingkat Kematangan Buah Dedi Nofiandi; Roslinda Rasyid; Aulia Maizola
Jurnal Riset Ilmu Kesehatan Umum dan Farmasi (JRIKUF) Vol. 3 No. 1 (2025): Januari : Jurnal Riset Ilmu Kesehatan Umum dan Farmasi (JRIKUF)
Publisher : LPPM STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/jrikuf.v3i1.533

Abstract

Blewah fruit (Cucumis melo L.) is a plant that contains β-carotene. β-caroten is a natural organic pigment that is yellow, orange and red orange brown which is found in vegetables and fruits. The research was also aimed to see difference of beta caroten on the Blewah fruit (Cucumis melo L.) based on the level of maturity of the fruit namely law and ripe. Each sample was extracted by using maceration method using aceton as as solvent, more over. Aceton extract was saponified matter and re-extracted using petroleum eter. Qualitative test using thin layer chromatography method with eluent n-hexane: aseton (9:1) and quantitative test using uv-vis spectrophotometry method at a maximum absorption wavelength of 479 nm. The qualitative test showed that law blewah and ripe blewah identified as containing beta caroten with an Rf 0,7. The quantitative analysis showed that beta caroten in young blewah and ripe fruit blewah 0,237 % b/b ± 0,0009 % b/b with a coefficient of variation 0,3861 % and 0,387 % b/b ± 0,0009 % b/b with a coefficient of variation 0,2366 %. The results show higest level of beta caroten obtained ripe fruit blewah followed by young blewah. The results of the residual standard deviation (SBr) 0,00414 µg/mL, limit of detection (BD) 2,28308 µg/mL and limit of quantitation with the regression equation y = -0,0012 + 0,00544x. Analysis method using t indenpenden (SPSS 25) showed the significant difference in each young blewah and ripe fruit blewah (p<0,005). Based on the levels obtained, the ripe blewah fruit can be used as a source of vitamin A.
Penetapan Kadar Beta Karoten pada Daun Sirih Hijau (Piper Betle L.) Berdasarkan Ketinggian Tempat Tumbuh Dedi Nofiandi; Hazli Nurdin; Sulha Ananda Putri
Jurnal Riset Ilmu Kesehatan Umum dan Farmasi (JRIKUF) Vol. 4 No. 1 (2026): Januari : Jurnal Riset Ilmu Kesehatan Umum dan Farmasi (JRIKUF)
Publisher : LPPM STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/jrikuf.v4i1.953

Abstract

Research has been conducted on determining beta carotene levels in green betel leaves (Piper betle L.) based on the altitude at which they grow. The purpose of this study was to determine the levels and differences in beta carotene levels in green betel leaves based on the altitude where it grows. This research consists of qualitative analysis and quantitative analysis. Qualitative analysis with thin layer chromatography and phytochemical screening tests on the extract, while quantitative analysis with UV-Visible spectrophotometry. The results of qualitative analysis with thin layer chromatography showed that green betel leaf extract contains beta carotene compounds obtained Rf value 0.48 and pure beta carotene Rf value 0.48. Other qualitative analysis with phytochemical screening tests on the three sample acetone extracts originating from the Alahan Panjang, Solok and Pasir Jambak areas contained terpenoid, phenolic, flavonoid compounds and did not contain saponin and alkaloid compounds. The results of quantitative analysis obtained the maximum absorption wavelength of beta carotene is 481 nm with the average beta carotene content in green betel leaves of Alahan Panjang, Paninggahan, Solok Regency, and Pasir Jambak respectively, namely 113.845 mg/100 g, 129.98 mg/100 g, and 7.530 mg/100 g. Data analysis using the One Way Anova test (SPSS 31.0) showed a significant effect of the height of the growing area on the beta carotene content of green betel leaves (P < 0.05).
Co-Authors Aulia Maizola Hazli Nurdin Roslinda Rasyid Sulha Ananda Putri