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All Journal Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Haj Ali, Mayadah A.
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Agriculture, Biological Sciences & Forestry

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Analysis of sesame phyllody disease using transmission electron microscopy and intersimple sequence repeat-polymerase chain reaction Haj Ali, Mayadah A.; Kheder, Ahmed A.; Hamwieh, Aladdin
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12584-95

Abstract

Sesame phyllody disease caused by phytoplasma has been identified in Egypt as a devastating phytopathogenic mollicute, However, the genetic diversity of phytoplasma infecting sesame plants in Egypt, as revealed by Intersimple Sequence Repeats-Polymerase Chain Reaction (ISSRs-PCR), has not been fully explored. During 2021–2022, sesame plants exhibiting virescence, phyllody, proliferation, and witches’ broom symptoms were observed in various fields across the Faiyoum, Luxor, and Beheira governorates in Egypt. This study aims to identify phytoplasma using transmission electron microscopy (TEM) and ISSRs-PCR. The methodology includes sampling, pathogenicity testing, TEM analysis of ultrathin sections, nested polymerase chain reaction (nested-PCR) targeting the 16S rRNA gene, and clustering analysis using 15 ISSRs primers. Phytoplasma was successfully transmitted to healthy plants through grafting and dodder transmission, with success rates of 80% and 90%, respectively. TEM analysis revealed polymorphic phytoplasma bodies and extensive phloem necrosis. Nested-PCR produced 1250 bp amplicons for all isolates. Phylogenetic analysis showed that the Beheira isolate (“OP185273.1”) is closely related to the peanut WB group. DNA polymorphic fragments ranged from 1 to 10 per profile, with fragment sizes between 150 and 1500 bp. Cluster analysis revealed that only two isolates (Faiyoum and Beheira) clustered together. This study demonstrates the effectiveness of ISSR-PCR in detecting and analyzing phytoplasma infecting sesame plants in Egypt.
Molecular Characterization of Mungbean yellow mosaic India virus and Tomato leaf curl New Delhi virus in Sukoharjo and Magelang Regencies, Indonesia Surwadinata, Antama; Putro, Ananda Restu; Pradhana, Adhi; Shalihah, Filzah; Pancasona, Muhammad Garda; Saputra, Rachmad; Haj Ali, Mayadah A.; Santosa, Adyatma Irawan
Jurnal Perlindungan Tanaman Indonesia Vol 29, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.101371

Abstract

Diseases caused by Begomoviruses in black-eyed pea (Vigna unguiculata subsp. unguiculata) and cucumber (Cucumis sativus) are remains insufficiently explored in Indonesia. One symptomatic black-eyed pea and two cucumber samples were collected from Sukoharjo and Magelang Regencies, respectively, to be molecularly tested using Krusty/Homer and SPG1/SPG2 primer pairs for universal detection of Begomoviruses. NCBI BLAST analysis on the obtained nucleotide sequences confirmed mungbean yellow mosaic India virus (MYMIV, Begomovirus vignaradiataindiaense) infection in black-eyed pea sample and tomato leaf curl New Delhi virus (ToLCNDV, Begomovirus solanumdelhiense) infection in both cucumber samples. Sequences of partial AV1, and partial AC1 and AC2 genes of the three isolates were registered with accession nos. PQ539469-71 and PQ539476-78, respectively, in NCBI GenBank. No recombination signal was detected in the sequences of the new isolates according to scan by Recombination Detection Program (RDP v.5.30). In the phylogenetic trees built by MEGA 11 software with Tamura-Nei parameter model, MYMIV H-2 as well as ToLCNDV N-8 and V-97 isolates shared basal nodes with Indonesian isolates, indicated their close genetic relationship with other isolates also found in the country. While expanding our information regarding genetic diversity of Begomoviruses, this study also reported the first cases of MYMIV in black-eyed pea in Indonesia and ToLCNDV in cucumber in Magelang, to the best of our knowledge.
Co-Authors Adyatma Irawan Santosa Hamwieh, Aladdin Kheder, Ahmed A. Pancasona, Muhammad Garda Pradhana, Adhi Putro, Ananda Restu Saputra, Rachmad Shalihah, Filzah Surwadinata, Antama