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All Journal Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Sarifan, Gracyana Putri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Other

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Penurunan Total Coliform Mata Air dengan Desain Biofilter Limbah Organik Djawa, Viennye Wilhelmina; Sarifan, Gracyana Putri; Tahu, Maria Astin Rosari; Semiun, Chatarina Gradict
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.555

Abstract

Unconserved springs tend to be polluted by various organic wastes. Accumulation of organic waste causes water to be contaminated with coliform bacteria, thus reducing water quality. Biofilter technology can be applied to reduce the number of coliform bacteria. This study aimed to determine the ability of BioFilter K4Ser-Col (moringa seeds, rice husks and kesambi charcoal) to reduce total coliform in spring water. The research location was at the Riumata Spring, Nekbaun village, West Amarasi sub-district, Kupang district. The work consisted of preparing the BioFilter K4Ser-Col media, taking water samples, and testing MPN coliform. MPN coliform testing was done twice before and after the water filtration on the BioFilter K4Ser-Col media. The study's results showed a decrease in the MPN value before and after filtering. The MPN coliform value before filtering was 1600 MPN/100 mL. After filtered water on the BioFilter K4Ser-Col media, it decreased to 23.23 MPN/100 mL. This value had met the national water quality standards for the class I category based on PP No. 22 of 2021. Thus, the use of BioFilter K4Ser-Col media could greatly reduce the number of coliform bacteria in water.
Kualitas Bakteriologi (Fecal Coliform) Mata Air Desa Toobaun Kabupaten Kupang Sarifan, Gracyana Putri; Semiun, Chatarina Gradict; Mamulak, Yulita Iryani
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.530

Abstract

Springs are groundwater that comes out on its own to the surface of the earth. Water sources originating from springs are generally suitable for direct consumption because they undergo natural purification. However, the high demand for clean water causes springs to experience anthropogenic pressure, which can reduce the quality of spring water. The study aimed to determine the Bacteriological quality of spring water in Toobaun village. The method used in this study was the Most Probable Number (MPN), using a series of five tubes with a variety of 5-5-5. The test included three stages, namely the prediction test, the confirmation test, and the complete test. The data obtained were presented in the form of a table, then compared with the MPN table value and analyzed descriptively by comparing the MPN value with the water quality standard, namely PP No. 22 of 2021 (class I). The results of the study showed that the Fecal Coliform value found was 220 MPN/100ml and did not meet the quality standards of PP No. 22 of 2021 (Class I). In the complete test using EMBA media, three types of bacteria were found that had characteristics similar to Escherichia coli and Salmonella sp. Klebsiella sp. Based on the results of water testing at the Toobaun spring, it cannot be consumed directly without first being processed.
Co-Authors Chatarina Gradict Semiun Djawa, Viennye Wilhelmina Tahu, Maria Astin Rosari Yulita Iryani Mamulak