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Jain, Sanjay
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Identification and Antifungal Susceptibility Pattern of Candida Isolates Recovered from Urine and Blood Specimens from Patients Admitted in Wards of a Tertiary Care Hospital, North Delhi Jain, Sanjay; Khatri, Shilpa; Kumar, Manoj; Bharara, Tanisha; Yadav, Abhishek
Journal of Epidemiology and Public Health Vol. 9 No. 2 (2024)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/jepublichealth.2024.09.02.08

Abstract

Background: Candida species are responsible for various clinical infections ranging from mucocutaneous infection to life-threatening invasive diseases. Increased resistance to antifungal drugs during the last decade has become a serious concern. Therefore, identification of Candida up to species level and its antifungal susceptibility testing is very important in the management of Candida infections. This study aimed to identify these organisms and study their susceptibility patterns.Subjects and Method: A retrospective study was conducted over 9 months (July 2022 to March 2023) from urine and blood samples collected from 80 IPD patients admitted to various wards of Hindu Rao Hospital. The samples were selected based on their growth on blood agar. The variables of interest are the different species of Candida and susceptibility to antibiotics. Identification of Candida species was done by Gram stain, Germ tube formation test, color on HiCrome Candida agar medium, chlamydospore formation on corn meal agar, and VITEK 2 Compact System. The MICs were interpreted according to the CLSI guidelines 2022.Results: 47 and 33 of urine and blood cultures were positive for Candida species respectively. Most of the isolates were from the Paediatric ward (28.75%), followed by the Medicine ward (27.5%). The most common species was C. tropicalis (56.25%) followed by C. albicans (23.75%). Most species of Candida were sensitive to amphotericin B, fluconazole, voriconazole, caspofungin, micafungin, and flucytosine except Candida albicans which showed 100% resistance to amphotericin B and Candida krusei which showed 66% sensitivity to voriconazole and 33% to caspofungin.Conclusion: Candida colonization has a considerable prevalence among patients hospitalized in our hospital. The species identification of Candida isolates along with their antifungal susceptibility pattern can help the clinician in better treatment of patients with candiduria and candidemia. Keywords: Candida, bloodstream infection, minimum inhibitory concentration
Prevalence of Infections Caused by Hepatitis B Virus, Hepatitis C Virus and Their Co-Infection in A Tertiary Care Centre, Delhi, India Kumar, Manoj; Jain, Sanjay
Journal of Epidemiology and Public Health Vol. 10 No. 1 (2025)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/jepublichealth.2025.10.01.08

Abstract

Background: Hepatitis B and C are the most dominant causes of viral hepatitis, leading to both acute and chronic infections. This study was done to determine the magnitude of the prevalence of Hepatitis B and C and their co-infection.Subjects and Method: A retrospective cross-sectional observational study was conducted in the Department of Microbiology, North DMC Medical College and Hindu Rao Hospital, Delhi, India from July 2023 to June 2024. All blood samples received from indoor and outdoor patients from all departments were tested by Monolisa HBsAg Ultra and anti-HCV(Ag-Ab Ultra V2) Monolisa by ELISA method. The variables studied were age, sex, in-patient/out-patient, and type of ward. The data was analyzed using the Chi-Square test and Mann-Whitney test.Results: Of a total of 20,000 blood samples received, 5,358(26.80%) samples were from inpatient department/IPD (2,210 female, 3,148 male) and 14642 (73.20%) and outpatient department/OPD (9,201 female, 5,441 male). 422 samples (2.11%) were positive for HBsAg (210 IPD -77 female, 133 male; 212 OPD - 121 female, 91 male) and 614 (3.07%) for anti-HCV (408 IPD - 135 female, 273 male; 206 OPD - 100 female, 106 male). Co-infection of Hepatitis B and C viruses was seen in 40 patients, 31 IPD (10 female, 21 male) and 9 OPD (1 female, 8 male). Maximum cases were seen from medicine wards among IPD patients of HBsAg positive, anti-HCV positive, and co-infection and in the age group 41-60 years in all three categories.Conclusion: Screening for HBV and HCV infection is compulsory so that preventive measures are implemented to improve public health.
Identification and Antifungal Susceptibility Pattern of Candida Isolates Recovered from Urine and Blood Specimens from Patients Admitted in Wards of a Tertiary Care Hospital, North Delhi Jain, Sanjay; Khatri, Shilpa; Kumar, Manoj; Bharara, Tanisha; Yadav, Abhishek
Journal of Epidemiology and Public Health Vol. 9 No. 2 (2024)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/jepublichealth.2024.09.02.08

Abstract

Background: Candida species are responsible for various clinical infections ranging from mucocutaneous infection to life-threatening invasive diseases. Increased resistance to antifungal drugs during the last decade has become a serious concern. Therefore, identification of Candida up to species level and its antifungal susceptibility testing is very important in the management of Candida infections. This study aimed to identify these organisms and study their susceptibility patterns.Subjects and Method: A retrospective study was conducted over 9 months (July 2022 to March 2023) from urine and blood samples collected from 80 IPD patients admitted to various wards of Hindu Rao Hospital. The samples were selected based on their growth on blood agar. The variables of interest are the different species of Candida and susceptibility to antibiotics. Identification of Candida species was done by Gram stain, Germ tube formation test, color on HiCrome Candida agar medium, chlamydospore formation on corn meal agar, and VITEK 2 Compact System. The MICs were interpreted according to the CLSI guidelines 2022.Results: 47 and 33 of urine and blood cultures were positive for Candida species respectively. Most of the isolates were from the Paediatric ward (28.75%), followed by the Medicine ward (27.5%). The most common species was C. tropicalis (56.25%) followed by C. albicans (23.75%). Most species of Candida were sensitive to amphotericin B, fluconazole, voriconazole, caspofungin, micafungin, and flucytosine except Candida albicans which showed 100% resistance to amphotericin B and Candida krusei which showed 66% sensitivity to voriconazole and 33% to caspofungin.Conclusion: Candida colonization has a considerable prevalence among patients hospitalized in our hospital. The species identification of Candida isolates along with their antifungal susceptibility pattern can help the clinician in better treatment of patients with candiduria and candidemia. Keywords: Candida, bloodstream infection, minimum inhibitory concentration
Prevalence of Infections Caused by Hepatitis B Virus, Hepatitis C Virus and Their Co-Infection in A Tertiary Care Centre, Delhi, India Kumar, Manoj; Jain, Sanjay
Journal of Epidemiology and Public Health Vol. 10 No. 1 (2025)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/jepublichealth.2025.10.01.08

Abstract

Background: Hepatitis B and C are the most dominant causes of viral hepatitis, leading to both acute and chronic infections. This study was done to determine the magnitude of the prevalence of Hepatitis B and C and their co-infection.Subjects and Method: A retrospective cross-sectional observational study was conducted in the Department of Microbiology, North DMC Medical College and Hindu Rao Hospital, Delhi, India from July 2023 to June 2024. All blood samples received from indoor and outdoor patients from all departments were tested by Monolisa HBsAg Ultra and anti-HCV(Ag-Ab Ultra V2) Monolisa by ELISA method. The variables studied were age, sex, in-patient/out-patient, and type of ward. The data was analyzed using the Chi-Square test and Mann-Whitney test.Results: Of a total of 20,000 blood samples received, 5,358(26.80%) samples were from inpatient department/IPD (2,210 female, 3,148 male) and 14642 (73.20%) and outpatient department/OPD (9,201 female, 5,441 male). 422 samples (2.11%) were positive for HBsAg (210 IPD -77 female, 133 male; 212 OPD - 121 female, 91 male) and 614 (3.07%) for anti-HCV (408 IPD - 135 female, 273 male; 206 OPD - 100 female, 106 male). Co-infection of Hepatitis B and C viruses was seen in 40 patients, 31 IPD (10 female, 21 male) and 9 OPD (1 female, 8 male). Maximum cases were seen from medicine wards among IPD patients of HBsAg positive, anti-HCV positive, and co-infection and in the age group 41-60 years in all three categories.Conclusion: Screening for HBV and HCV infection is compulsory so that preventive measures are implemented to improve public health.
Co-Authors Bharara, Tanisha Khatri, Shilpa Kumar, Manoj Yadav, Abhishek