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All Journal Health Care : Journal of Community Service Advance Sustainable Science, Engineering and Technology (ASSET)
Reny Pratiwi
Universitas Setia Budi
Chemistry Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Health Professions Industrial & Manufacturing Engineering Medicine & Pharmacology

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Comparing Conventional and Modern Methods for The Phycocyanin Extraction from Spirullina sp Dian Marlina; Desi Purwaningsih; Reny Pratiwi; Ryan Werytama Saputra; Widiastuti Setyaningsih; Supriyono Supriyono
Advance Sustainable Science Engineering and Technology Vol. 6 No. 3 (2024): May - July
Publisher : Science and Technology Research Centre Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v6i3.907

Abstract

Spirulina platensis, a blue-green algae abundant in tropical regions, is rich in minerals, vitamins, fibers, and pigments, with low nucleic acid content. It has unique chromoproteins called phycobiliproteins, notably phycocyanin, used in various applications. This study aims to optimize phycocyanin extraction using different solvents (distilled water and sodium phosphate buffer pH 6.7) and methods (freeze-thaw and sonication). Spirulina platensis biomass was extracted in both solvents, then some of them was freeze for 24 and 48 hours followed by thawing overnight. The other was sonicated for 2.5 minutes, 50 Hz then soaked for 1, 2, and 3 hours. All of the samples were centrifuged at 6000 rpm for 10 minutes and the absorbance was measured using a UV-Vis spectrophotometer at wavelengths of 280, 620, and 650 nm. with freeze-thawing for 48 hours yielded the highest phycocyanin concentration (0.55%), with a yield of 11.07 and purity of 0.21. Sonication improved phycocyanin concentration, yield, and purity significantly, yielding 1.108, 25.85, and 0.26, respectively.
Pentingnya Mengetahui Golongan Darah Sejak Dini sebagai Identitas Diri Rumeyda Chitra Puspita; Lucia Sincu Gunawan; Reny Pratiwi
Health Care : Journal of Community Service Vol. 4 No. 1 (2026)
Publisher : Rena Cipta Mandiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62354/healthcare.v4i1.185

Abstract

Blood group identification from an early age is an important aspect of health awareness and serves as a personal identity that can support emergency management, such as anemia and blood transfusion needs. This community service activity aimed to improve students’ knowledge and awareness regarding the importance of knowing their blood group through health education and direct examination. The methods included health education on the importance of blood groups and anemia prevention, followed by ABO and Rhesus blood group examination using the agglutination method with Anti-A, Anti-B, Anti-AB, and Anti-D antisera from capillary blood samples. The subjects consisted of elementary and junior high school students, including 60 students from SMP 12 Surakarta, 50 students from SMP 18 Surakarta, and 50 students from MIAI Grobogan. The results showed that most respondents in SMP 12 (70.0%) and SMP 18 (88.0%) were aged 13–15 years, while MIAI Grobogan was dominated by students aged 11–12 years (56.0%). The level of knowledge was still low in SMP 12 (23.3%) and SMP 18 (36.0%), but higher in MIAI Grobogan (64.0%). Most students showed a neutral response toward the examination, although fear was higher in MIAI Grobogan (60.0%). Overall, blood group distribution was dominated by type O (30.6%) and A (27.5%), followed by B (25.6%) and AB (16.3%). In conclusion, this activity was effective in improving students’ knowledge and awareness regarding the importance of blood group identification; however, continuous educational reinforcement is still needed.
Co-Authors Desi Purwaningsih Gunawan, Lucia Sincu Marlina, Dian Rumeyda Chitra Puspita Ryan Werytama Saputra Supriyono Supriyono Widiastuti Setyaningsih