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All Journal Jurnal Florea
Haris Rizki Maulana
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Comparison of Blood Glucose Examination Results Using Serum and Plasma Sodium Floride (NaF) in Hyperglycemia Samples With Examination Delay Time of 30 Minutes and 1 Hour Ellies Tunjung Sari Maulidyanti; Lihabi Lihabi; Nur Vita Purwaningsih; Rahma Widyastuti; Haris Rizki Maulana
Florea : Jurnal Biologi dan Pembelajarannya Vol. 11 No. 1 (2024)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v11i1.21930

Abstract

Blood glucose level examination until now often cannot be done immediately after the sample is taken. Glycolysis in the blood can occur in vitro, thus affecting the examination results. The addition of anticoagulants such as NaF aims to inhibit the in vitro glycolysis process. Several studies have described the effects of anticoagulants in maintaining blood sugar levels in examination samples. However, the results presented still have some differences. This study aims to determine the difference in blood glucose levels using serum and plasma NaF in hyperglycemia samples with a delay of 30 minutes and 1 hour. The type of research used in this study is analytical observational with a cross-sectional approach that is a comparative study. The population in this study were all outpatients at the Muhammadiyah Hospital in Surabaya. This study was conducted at the Muhammadiyah Hospital Laboratory in Surabaya, during the study in March-April 2024. The results of this study obtained blood glucose levels using either serum or NaF plasma in hyperglycemic and non hyperglycemic samples examined with a delay of 30 minutes and 1 hour. The Greenhouse-Geisser Sig value was 0.000 (<0.05) which means there is a real (significant) difference
Comparison of Albumin Level Test Results with Serum and EDTA Plasma Samples : Comparison of Albumin Level Test Results with Serum and EDTA Plasma Samples Rinza Rahmawati Samsudin; lihabi, lihabi; Fitrotin Azizah; Haris Rizki Maulana
Florea : Jurnal Biologi dan Pembelajarannya Vol. 12 No. 2 (2025)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v12i2.22995

Abstract

Blood albumin level testing is an important parameter in assessing nutritional status and liver function. In clinical laboratory practice, the use of serum or EDTA plasma as biological samples remains a subject of debate, particularly regarding the efficiency and accuracy of results. This study aims to compare albumin level test results using serum and EDTA plasma samples. Blood samples were collected from 20 participants and analyzed using spectrophotometry. The average albumin level in serum samples was 4.285 g/dL, while in EDTA plasma it was 4.280 g/dL. The Shapiro-Wilk normality test indicated a normal data distribution (p>0.05), and the paired t-test yielded a p-value of 0.804 (>0.05), indicating no significant difference between the two sample types. These findings suggest that EDTA plasma can serve as an alternative to serum in albumin testing, with the advantage of improved processing efficiency.
Co-Authors Ellies Tunjung Sari Maulidyanti Fitrotin Azizah Lihabi Lihabi Lihabi, Lihabi Nur Vita Purwaningsih, Nur Vita Rahma Widyastuti Rinza Rahmawati Samsudin