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All Journal IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Jurnal Kesehatan Farmasi
Tania, Alinda
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Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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The Effect of Oxidative Stress on Growth and Antioxidant Activity of Microalgae Oocystis sp Musifa, Eva; Warni, Mega; Talib, Ramanisa Muliani; Dharma, Abdi; Mardiah, Elida; Harvianti, Yuniar; Indah, Indah; Tania, Alinda
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 10, No 1 (2025): February 2025
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v10.i1.30

Abstract

This study was conducted to see the effect of growth and antioxidant activity of microalgae Oocystis sp before and after adding NaOCl to Growmore medium combined with urea. Antioxidant activity test was conducted by calculating IC50 spectrophotometrically with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Oocystis sp extract was obtained by sonication and maceration process with methanol solvent. The results showed the best growth curve for the growth of microalgae Oocystis sp in a mixture of Growmore medium 0.02% b/v and Urea 0.002% b/v. These results also showed a decrease in Oocystis sp growth in a mixture of Growmore medium 0.02% b/v and Urea 0.002% b/v with the lowest growth decrease in the addition of NaOCl with a concentration of 0.01%. The IC50 value of Oocystis sp extract in methanol solvent has high antioxidant activity in Growmore 0.02% b/v + Urea 0.002% b/v medium, which is 55.5862 mg/L. It can be concluded that NaOCl affects the growth and antioxidant activity of microalgae Oocystis sp, but at certain concentrations NaOCl experienced a negative effect.Keywords: Microalgae, Oocytis sp, Oxidative Stress, Growth, Antioxidant
PROFIL METABOLIT DAN PENENTUAN NILAI SUN PROTECTION FACTOR IN VITRO EKSTRAK ETANOL DAUN MURBEI (Morus alba L.) SEBAGAI TABIR SURYA Tania, Alinda; Agustin, Yeni
Jurnal Kesehatan Farmasi Vol 7 No 1 (2025): Jurnal Kesehatan Farmasi
Publisher : Jurusan Farmasi, Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jkpharm.v7i1.2878

Abstract

Besarnya kemampuan tabir surya untuk melindungi kulit ditentukan melalui nilai SPF yang menyatakan lamanya kulit seseorang berada dibawah sinar matahari tanpa mengalami sengatan surya. Penelitian ini bertujuan untuk memperkirakan nilai SPF dari ekstrak etanol daun murbei (Morus alba L.). Daun murbei memiliki kandungan alkaloid, flavonoid, polifenol dan terpenoid yang mempunyai peranan sebagai antioksidan. Penelitian ini bertujuan untuk menetahui potensi penggunaan ekstrak etanol daun murbei sebagai tabir surya. Ekstraksi dilakukan dengan metode maserasi dan penentuan nilai SPF dilakukan secara in vitro menggunakan metode Spektrofotometri UV-Vis. Absorbansi ekstrak daun murbei diukur pada panjang gelombang sinar UV-B yaitu 290-320 nm. Penentuan nilai SPF didasarkan pada persamaan Mansur. Berdasarkan hasil penelitian, diketahui bahwa daun murbei terbukti memiliki potensi sebagai tabir surya dengan nilai SPF rata-rata 3,19 yang terkategori minimal pada konsentrasi 300 ppm dan sebesar 6,27 yang terkategori ekstra pada konsentrasi 600 ppm. Hasil penelitian ini dapat menjadi dasar dikembangkannya produk tabir surya berbahan dasar ekstrak etanol daun murbei.
Co-Authors Abdi Dharma Elida Mardiah Harvianti, Yuniar Indah Indah Musifa, Eva Talib, Ramanisa Muliani Warni, Mega Yeni Agustin