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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Global Health Management Journal
Nisa, Mutia Khoirun
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Exploring the Antioxidant and Anti-proliferative Effects of Murraya koenigii (L.) Leaves Methanol Extract on T47D Breast Cancer Cell Lines Nisa, Mutia Khoirun; Salamah, Rohmi; Wijayanti, Nastiti
GHMJ (Global Health Management Journal) Vol. 8 No. 1 (2025)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-811204

Abstract

Background: Murraya koenigii (Curry) leaves are herbal plants that have bioactive compounds such as phenolics, flavonoids, and alkaloids that function as antioxidants and anti-cancers. Aims: This study aimed to quantitatively determine the bioactive compounds in curry leaves by calculating phenolic, flavonoid, and alkaloid content. Its potential as an antioxidant and anti-proliferative compound in T47D breast cancer cell lines was also analyzed. Methods: Murraya koenigii leaves extract was obtained by maceration using 80% methanol (1:5 w/v), then the antioxidant and anti-proliferative test was carried out using the 1,1-Diphenyl-2-Pycrylhidracyl (DPPH) and MTT (-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay, respectively, followed by flow cytometry to determine the apoptotic activity of the extract on T47D breast cancer cell lines. Results: The results for the bioactive compounds in the methanol extract of Murraya koenigii leaves were 156.62±1.49 mg/g phenolics, 99.19±0.25 mg/g flavonoids, and 2.90±0.01 mg/g alkaloids. The IC50 value for antioxidant activity was 25.058±2.2 μg/ml and showed an anti-proliferative effect on T47D cells in a dose-dependent manner with IC50 74.71±5.45 μg/ml for cytotoxicity. Furthermore, flow cytometry showed that a concentration of 1/16 IC50 has the best results for apoptosis. Conclusion: The methanol extract of Murraya koenigii leaves has the potential as an antioxidant and anti-proliferative agent and can induce apoptosis of T47D cells.
Nanochitosan from Manufacture Shells of Mud Crab (Scylla sp.) and Its Application as Acne Patch Luthfiyana, Novi; Ratrinia, Putri Wening; Nusaibah, Nusaibah; Nisa, Mutia Khoirun
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 2 (2024): August 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.928

Abstract

Chitosan is a potential raw material for marine cosmetics with antibacterial,anti-inflammatory properties and can stimulate collagen synthesis in theskin. This research aimed to obtain nano-sized chitosan (nanochitosan) frommud crab (Scylla sp.) shells and apply it as a cosmetic acne patch. Theparameters used to characterize nanochitosan are particle size, polydispersityindex, zeta potential and morphology. Meanwhile, the parameters of the Acnepatch produced are characterized by physical appearance and ance bacterialactivity. The results revealed that nanochitosan had an average size of 35.20±1.66 nm, the highest intensity of 11.70±1.25, polydispersity of 0.274±0.10,and zeta potential of 42.9±1.41 mV. The morphology of nanochitosan is in theform of broken rods, not hollow and irregular but uniform. Its elements includecarbon, oxygen, magnesium, aluminum, phosphorus, and calcium. The acnepatches resulting from the addition of 0 mg (K0), 50 mg (K1), 100 mg (K2),and 150 mg (K3) were opaque white, slightly transparent, smooth, elastic,and odorless. The thickness varies, namely, 0.014 ± 0.002 mm (K0), 0.017 ±0.002 mm (K1), 0.022 ± 0.004 mm (K2), and 0.031 ± 0.005 mm (K3). Theweight variations were 0.019 ± 0.001 mg (K0), 0.026 ± 0.005 mg (K1), 0.033± 0.002 mg (K2), and 0.047 ± 0.013 mg (K3). The moisture loss varied,namely, 7.525 ± 0.054% (K0), 3.201 ± 0.487% (K1), 2.741 ± 0.279% (K2), and2.017 ± 0.290% (K3). Acne patches K1, K2, and K3 were proven to be able toinhibit the activity of acne bacteria Propionibacterium acnes (9.5 ± 0.25 mm,10.67 ± 0.76 mm, and 9.17 ± 0.76 mm), Staphylococcus epidermidis (9.33 ±1.15 mm, 13.67 ± 2.02 mm, and 8.67 ± 1.89 mm), and S. aureus (10.33 ±0.25 mm, 11.83 ± 0.76 mm, and 8.66 ± 0.76 mm). This research succeededin obtaining chitosan from crab shells (Scylla sp) in nano size and acnepatches with the addition of nanochitosan effectively inhibited the growth ofacne bacteria.
Co-Authors Nastiti Wijayanti Novi Luthfiyana Nusaibah Nusaibah Putri Wening Ratrinia Salamah, Rohmi