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All Journal The Journal of Experimental Life Sciences (JELS) Biotropika
Wahyuningsih, Nadia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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Phytochemical Profiles, DPPH Radical Inhibition Effectiveness, and Cytotoxic Potential of Polyherbal Plants in T47D Cell Line Wahyuningsih, Nadia; Rahayu, Sri; Rifa'i, Muhaimin
The Journal of Experimental Life Science Vol. 15 No. 1 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.01.02

Abstract

Breast cancer subtypes include luminal A, luminal B, HER2, triple-negative, and normal-like. Antioxidants, ROS, and cancer have a close relationship. Cancer cells exhibit an elevated ROS level, which can be used for selective cell-based therapeutic targeting. Therefore, developing potential therapeutic potential therapeutic options is crucial. Herbal medicine has gained much interest in treating cancer. Indian jujube (Ziziphus mauritiana), cumin (Nigella sativa), turmeric (Curcuma longa), soursop (Annona muricata), and meniran (Phylanthus niruri) potentially have a role as anticancer agents and were used as alternative medicine. This study aimed to determine the phytochemicals total content, DPPH radical inhibition, and cytotoxicity of the polyherbal plants to the T47D cell line. The antioxidant analysis was conducted using DPPH assay, and the phytochemicals analysis was determined using total flavonoid (TFC), phenolic (TPC), and alkaloid (TAC) content. The cell viability assessment was performed using the WST-1 method. The antioxidant analysis revealed that the IC50 value of polyherbal medicine is 213.65 µg.ml-1. The phytochemical analysis showed that the highest content is the flavonoid compared to others, with a value of 138.986 mgQE.mg-1. Other than that, the cell viability assessment showed that the IC50 of the herbal medicine is 480.8 µg.mL-1. These findings indicated that combining turmeric, cumin, meniran, Indian jujube, and soursop has moderate cytotoxicity against the T47D cell line since it can reduce T47D cell line viability at high doses. Keywords: antioxidant, breast cancer, herbal medicine, phytochemicals, T47D cell line.
Virtual Based Screening of Rosmaniric Acid and Its Derivatives as Potential Anticancer Approach Targeting HIF-1a Naufal, Achmad Hanif; Wachid, Nisa Nabila Aufa; Kamila, Fairuz Sarah; Wahyuningsih, Nadia; Ilmiyah, Silvi Zakiyatul; Nafisah, Wirdatun; Fatchiyah, Fatchiyah
Biotropika: Journal of Tropical Biology Vol. 13 No. 1 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.01.03

Abstract

Cancer is one of the leading causes of death in the world. Rosmarinic acid becomes one of the important polyphenolic compounds. It includes derivatives of two amino acids, caffeic acid from phenylalanine and 3,4-dihydroxyphenyl-lactic acid from tyrosine, secondary metabolites found in many herbal plants from the subfamily Lamiaceae and Boraginaceae. Rosmarinic acid works in inhibiting the occurrence of metastases and tumor formation. In this study, we evaluated rosmarinic acid and its derivatives through study literature. The compounds were downloaded from the PubChem database, while the three-dimensional structure of HIF-1a was retrieved from RCSB PDB. The biological activity was analyzed using the PASS server, and the cytotoxic effect was predicted using CLC-Pred., The pharmacological properties of bioactive compounds were analyzed using SwissADME. The compounds and HIF-1a were prepared using PyRx 0.8 version and Discovery Studio software, docked specifically using Autodock Vina, visualized using Discovery Studio software, and molecular dynamic simulation using CABS-flex 2.0. Rosmarinic acid and its derivative showed an inhibition with the highest affinity value of compound against HIF-1a are control (-6.9 kcal/mol), Rosmarinic acid (-6.4 kcal /mol), and Methyl rosmarinate (-6.3 kcal/mol). Rosmarinic acid could be expected to have activity as an inhibitor modulated at the post-translational level. Comprehensive and further analysis is required in future research based in vivo and in vitro for the development of cancer treatments.
Co-Authors fatchiyah . Ilmiyah, Silvi Zakiyatul Kamila, Fairuz Sarah Nafisah, Wirdatun Naufal, Achmad Hanif Rifa'i, Muhaimin SRI RAHAYU Wachid, Nisa Nabila Aufa