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Andi Muflihunnah
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Medicine & Pharmacology

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Analisis Kadar Astaxanthin Ekstrak Cangkang Udang Vannamei (Litopenaeus vannamei) Asal Kecamatan Malangke Barat Dengan Metode Spektrofotometri UV-Vis Aulia Nur Afifah; Harti Widiastuti; Andi Muflihunnah
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 1 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

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Abstract

Shrimp waste consisting of heads, shells, legs and tails contains several components such as protein, chitin, enzymes, minerals, carotenoids, and others. One type of carotenoid found in shrimp waste is astaxanthin. Astaxanthin is a carotenoid pigment with higher antioxidant activity compared to other β-carotene and vitamin E derivatives. The purpose of this study was to determine the levels of astaxanthin in the shells of vannamei shrimp (Litopenaeus vannamei) from West Malangke District. The astaxanthin compound contained in vannamei shrimp shells was extracted using the UAE method and liquid-liquid extraction. The percentage value of the reduction is 0.486%. Qualitative analysis used thin layer chromatography (TLC) and quantitative analysis using UV-Vis spectrophotometry. TLC analysis on the sample obtained an Rf value of 0.254. Analysis by UV-Vis spectrophotometry at a maximum wavelength of 203 nm obtained y = 0.1513x – 0.0819, correlation coefficient (r) = 0.9947, Vx0 = 4.64%, and the average level of astaxanthin = 73.2 %.
Uji Aktivitas Antioksidan Ekstrak Etanol Buah Mangsi (Phyllanthus reticulatus) dengan Menggunakan Metode DPPH Nurmayada wahab; Andi Muflihunnah; Rahmawati Rahmawati
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 1 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

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Abstract

Antioxidants are compounds that can inhibit free radical reactions in the body. Synthetic antioxidants are often used for food but their use is limited because they are toxic. This study aims to determine the antioxidant activity of mangosteen fruit samples. The method used in this test is the DPPH method. This test was carried out by adding 1 mL of the test solution with 4 mL of 40 ppm DPPH. The absorbance was measured with a UV-Vis spectrophotometer at a wavelength of 498 nm and the IC50 value was calculated based on the absorbance data. The calculation results show that there is antioxidant activity in the ethanol extract of mangosteen fruit (Phyllantus reticulatus) with an IC50 value of 2.278 ppm, that is, these results show very strong antioxidant activity, and quercetin as a comparison has a very strong antioxidant activity with an IC50 value of 4.184 ppm. Keywords: : Antioxidant, Ethanol Extract of Mangsi Fruit (Phyllanthu reticulatus), DPPH, Spectrophotometer UV-Vis
Co-Authors Aulia Nur Afifah Harti Widiastuti Nurmayada wahab Rahmawati Rahmawati