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Fernanda, MA Hanny Ferry
Universitas Nahdlatul Ulama Sidoarjo
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology

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Journal : Journal of Research and Technology

 1 
ANALISIS KUALITATIF DAN KUANTITATIF CEMARAN LOGAM BERAT TIMBAL (Pb) DAN KADMIUM (Cd) PADA PRODUK KOSMETIK PENSIL ALIS MENGGUNAKANSPEKTROFOTOMETER SERAPAN ATOM (SSA) Arifiyana, Djamilah; Fernanda, MA Hanny Ferry
Journal of Research and Technology Vol 4, No 1 (2018)
Publisher : Universitas Nahdlatul Ulama Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (88.057 KB) | DOI: 10.5281/zenodo.2580443

Abstract

The aim of this study was to identify and determine the heavy metal content of lead (Pb) and cadmium (Cd) towards eyebrow pencil registered and not registered by the Food and Drug Supervisory Agency (BPOM). This research was conducted in several markets in Surabaya. Twelve eyebrow pencil items were investigated for lead (Pb) and cadmium (Cd). Simple reagents, included HCl, NaOH, KI, Na2S2O3 and NH4OH were used to identify the presence of these heavy metal first. The study results showed that samples of eyebrow pencil registered and unregistered by BPOM RI after being tested turned out to contain lead and cadmium in a range of 1.092-5,834 mg/kg and 0,054-0.243 mg/kg, respectively. The content is still within what is required based on the regulations of BPOM RI, < 20 mg/kg for lead content and < 5 mg/kg for cadmium content. In short, all the sample of eyebrow pencil contained lead (Pb) and cadmium (Cd) within what is required based on the regulations of BPOM RI.
VERIFIKASI LINIERITAS KURVA BAKU TESTOSTERON MENGGUNAKAN METODE ELISA (ENZYME-LINKED IMMUNOSORBENT ASSAY) Fernanda, MA Hanny Ferry; Sa'adi, Ashon; Sudjarwo, Sudjarwo
Journal of Research and Technology Vol 5, No 1 (2019)
Publisher : Universitas Nahdlatul Ulama Sidoarjo

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Abstract

ELISA (Enzyme-Linked Linked Immunosorbent Assay) is one of the quantitative analysis methods that is often used to determine the levels of active compounds in biological samples. In this study, we will verify the method of determining testosterone active compounds in blood and urine samples of female patients who have PCOS (Polycystic Ovary Syndrome) using the Human Testosterone ELISA Kit. Before conducting testosterone levels using ELISA, it is necessary to verify the linearity of the standard curve first to determine the effect of testosterone standard levels on the analyte response in the form of optical density. The linearity verification of the standard testosterone curve is calculated using linear regression calculations with the results of the coefficient of determination which is 0.978 with a value of α = 0.05. While for testing with the calculation of 4 parameter logistic (4PL), the results of the coefficient of determination are 0.999. Based on these results it can be concluded that the testosterone standard level has a significant influence on the response of optical density analytes.
Co-Authors Amalia, Anisa Riski Asroriyah, Heppy Cicik Herlina Yulianti Devianti, Vika Ayu Djamilah Arifiyana Elidya, Devi Kusumo, Galuh Gondo Manaheda, Novianti Ayu Qomaryah, Nurul Sa'adi, Ashon Sudjarwo Sudjarwo Umam, Muhammad Khotibul Wardani, Ratih Kusuma