Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal The Journal of Experimental Life Sciences (JELS) Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat
Haryadi, Nanang
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Social Sciences

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Isolation and Characterization of α-Amylase Enzyme on Brown Planthopper (Nilaparvata lugens Stal) On Rice Syakuro, Muhammad Yusuf Abdan; Haryadi, Nanang; Muhlison, Wildan; Sucipto, Irwanto
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.02

Abstract

Brown leafhopper (Nilaparvata lugens Stal) is an important pest in Indonesia that causes heavy losses. The rice was damaged by N. Lugens sucking plant liquid in the form of carbohydrates in the form of starch. Environmentally friendly control methods are needed to reduce the increasingly high use of inorganic pesticides. Control of N. lugens with biotechnology can be done by inhibiting the metabolic cycle in these insects. This method utilizes the proteins available in rice seeds to stop the performance of enzyme α-amylase in the digestive system of N. lugens. α-amylase is an enzyme that plays a role in the process of starch degradation so that it becomes a simpler form both in microorganisms. The characterization of α-amylase enzymes in insects is an important first step to the determination of appropriate protein inhibitors so that they can be used to produce pest-resistant Genetically Modified Organisms crops. The research was conducted in the Agrotechnology laboratory, University of Jember. The research began with isolation and purification of α enzymes from brown stems and then continued with testing of α-amylase activity. Test parameters include the effect of temperature, pH, and substrate concentration on the activity of the α-amylase enzyme. The results showed that supernatant extracted from brown planthoppers produces a clear zone in the agar medium, which means the activity of enzymes α-amylase in the hydrolysis of starch. The pH value of 6 provides the most optimum conditions for the activity of α enzymes. The α-amylase enzyme is able to work optimally in the temperature range of 30°C - 45°C, and experiences a decrease in activity when the temperature reaches 50°C. The α-amylase enzyme shows the ability to hydrolyze the amylase substrate to a concentration of 0.8 μg.μL-1. Keywords: α-amylase, Nilaparvata lugens Stal, Rice.
Nolobranti tourism development in Wangkal village Gading district of Probolinggo regency become agroeduwisata Haryadi, Nanang; Hari Purnomo; Wildan Muhlison; Titin Agustina
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 2 (2022): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/abdimastalenta.v7i2.8750

Abstract

Wangkal Village is one of the villages in Gading District of Probolinggo Regency that has agricultural potential that supports tourism development. Agricultural potential includes abundant water conditions so that people prefer to grow rice every year and be used for freshwater fish cultivation. Another potential that now exists in Wangkal village is the Nolobranti River Tubing tour. This tour offers the concept of tubing down the river along the village of Wangkal. This tour in the dry season is less running because the water discharge is reduced. The Wangkal village government wants to develop agricultural potential and serve as a center for education for the community and tourists who are traveling in Nolobranti. The problem of nolobranti tourism development, among others, this tourist spot only provides river tubing packages. Therefore, it is necessary for the development of nolobranti tourism to become a place of education in the field of agriculture by developing freshwater fisheries combined with rice farming and becoming an educational center for organic agricultural startups. The hope is that this tourist area will not only become a tourist transit, but also a place of education as an agroeduwisata. Agroeduwisata area will be able to offer tour packages, among others: farming various types of vegetables, getting to know sewage treatment, getting to know fish and livestock cultivation, making biopesticides, biofertilizers, packages feeding freshwater fish, airy tours about organic agricultural systems or rice minas, business motivation and other trainings. The results of the service activities show that the community has been able to produce biological products such as making biopesticides, learning the cultivation of black flies to process waste and freshwater cultivation combined with aquponics and mina padi (rice-fish integrated farming system).
Co-Authors Hari Purnomo Irwanto Sucipto, Irwanto Syakuro, Muhammad Yusuf Abdan Titin Agustina Wildan Muhlison, Wildan