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All Journal The Journal of Experimental Life Sciences (JELS) REKAYASA Jurnal Penelitian Pendidikan IPA (JPPIPA)
Zamzami, Ilham Misbakudin Al
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Electrical & Electronics Engineering Engineering Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics

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Journal : Jurnal Penelitian Pendidikan IPA (JPPIPA)

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Analysis of Protein Profile and Functional Groups in Biofilms in the Hypersaline Environment Zamzami, Ilham Misbakudin Al; Kilawati, Yuni; Ibdiah, Raudatul; Kurniawan, Andi
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4464

Abstract

Halophilic microorganisms include extremophiles that can live and develop in a hypersaline environment. Biofilm, as microecology, consists of a collection of microorganisms attached to a surface, forming an Extracellular Polymeric Substance (EPS) matrix. This study aims to analyze the protein profile and functional groups of biofilms that grow in hypersaline environments. The method used in this study is the SDS PAGE test to diagnose protein profiling and FTIR to analyze biofilm functional groups. The samples used to grow the biofilm came from salt ponds with different NaCl levels, namely 2%, 25%, and 40%. The results showed that each NaCl treatment had different functional groups in the biofilm samples. In the 2% NaCl treatment, there were peaks with solid intensity at the wavenumber 1100 cm-1, indicating the carboxylic acid functional group. In the 25% NaCl treatment, peaks with solid intensity at wavenumber 2250 cm-1 indicate the isocyanate functional group. In the 40% NaCl treatment, there were peaks with solid intensity at wavenumber 860 cm-1, indicating the alkene functional group. The protein analysis for each treatment shows the presence of pyruvate kinase protein in the biofilm with 2% NaCl. The 25% NaCl biofilm sample contained avidin protein, and the 40% NaCl biofilm sample contained tyrosine protein.
Analisis Dinamika Faktor Lingkungan Selama Proses Pengkristalan Garam di Greenhouse Salt Tunnel (Studi kasus di Pantai Utara dan Pantai Selatan, Jawat Timur, Indonesia) Amin, Abd Aziz; Yanuar, Adi Tiya; Pramudia, Zulkisam; Susanti, Yogita Ayu Dwi; Zamzami, Ilham Misbakudin AL; Salamah, Lutfi Ni’matus; Lestariadi, Riski Agung; Hakim, Lukman; Ardian, Gatot; Hanifuddin, Mokh; Kurniawan, Andi
Jurnal Penelitian Pendidikan IPA Vol 11 No 4 (2025): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i4.7131

Abstract

Salt is an essential commodity for both domestic use and industrial applications, with East Java Province in Indonesia playing a crucial role in national salt production. Traditional salt production methods, primarily based on natural evaporation, face challenges due to seasonal factors, particularly during the rainy season. This study investigates the environmental conditions affecting the salt crystallization process in the Greenhouse Salt Tunnel (GST) technology. Field experiments were conducted at two sites, Tuban Regency on the North Coast and Malang Regency on the South Coast, where key parameters such as air temperature, water temperature, humidity, wind speed, and evaporation rates were monitored three times daily. The findings showed that water temperatures within the GST ranged from 31 to 38°C on the North Coast and 26 to 38°C on the South Coast. Wind speeds varied between 1.5–2.4 m/s on the North Coast and 0.4–1.0 m/s on the South Coast, while humidity levels ranged from 54 to 80%. The GST system achieved a salt production rate of 6.4 kg/m² with NaCl content exceeding 94%, a significant improvement over traditional methods, which yield less than 70% NaCl. These results demonstrate that GST technology enhances both the quality and quantity of salt production, though design modifications are needed to address the dynamic coastal environment.
Co-Authors Amin, Abd Aziz Amin, Abd. Aziz Andi Kurniawan Ardian, Gatot Hanifuddin, Mokh Ibdiah, Raudatul Lestariadi, Riski Agung Lukman Hakim Pramudia, Zulkisam Salamah, Lutfi Nimatus Salamah, Lutfi Ni’matus Susanti, Yogita Ayu Dwi Yanuar, Adi Tiya Yuni Kilawati