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All Journal The Journal of Experimental Life Sciences (JELS) REKAYASA Jurnal Penelitian Pendidikan IPA (JPPIPA) Yumary: Jurnal Pengabdian kepada Masyarakat
Susanti, Yogita Ayu Dwi
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Social Sciences Other

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Analysis of Microbial Abundances in Biofilms and Water in Hypersaline Environments with Different NaCl Levels Zamzami, Ilham Misbakudin Al; Kilawati, Yuni; Pramudia, Zulkisam; Susanti, Yogita Ayu Dwi; Kurniawan, Andi
The Journal of Experimental Life Science Vol. 13 No. 2 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.02.07

Abstract

Biofilm is the dominant habitat for aquatic microbes that can grow in various aquatic conditions, including environments with extreme conditions. One of the extreme conditions found in aquatic ecosystems is a hypersaline environment. As part of aquatic biological resources, microbes can also live in this environment. However, although it is the predominant habitat of microbes, biofilms in hypersaline environments have yet to be widely explored. Understanding biofilms in hypersaline environments, especially salt ponds, will increase knowledge about microbial biofilms. The knowledge can open opportunities for using microbes as aquatic resources, such as aquaculture or environmental biotechnology. This study analyzed microbial abundance in biofilms and water in hypersaline environments. The water is from salt ponds on the South Coast of Malang Regency, Indonesia, with different salinity levels (2%, 25%, and 40%). Microbial abundance was analyzed using the culture method using several culture media (Modified Nutrient Agar, Modified Total Plate Agar, Mannitol Salt Agar, and Casein Medium Agar). The substrate for biofilm growth is HDPE, often used as a bottom coating for salt ponds and shrimp ponds. Moreover, this study also analyzed water quality parameters (pH, dissolved oxygen, turbidity, conductivity, total dissolved solids, temperature) during the biofilms' forming process. The results of this study indicate that the abundance of good microbes that grow in the biofilm or the water around the biofilm is strongly influenced by the type of media used for culturing. The main composition of the most influential media is peptone. The density of microbes in the biofilm is hundreds to thousands of times higher than that in the surrounding water. The results of this study also show that the presence of microbes in both water and biofilm affects the water quality properties in hypersaline environments. Keywords: aquatic ecosystem, biofilm, hypersaline, microbial ecology.
Analisis Environmental-DNA (E-DNA) Untuk Estimasi Jumlah Total Bakteri Pada Air Kolam dengan Sistem Recirculation Aquaculture System (RAS Kurniawan, Andi; Amin, Abd. Aziz; Guntur, Guntur; Susanti, Yogita Ayu Dwi; Wiryawan, Adam; Pramudia, Zulkisam; Okuda, Hideki; Yanuar, Adi Tiya
Rekayasa Vol 15, No 3: Desember 2022
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/rekayasa.v15i3.16844

Abstract

Catfish is an aquaculture commodity that has great potential to be developed in various regions in Indonesia. The success of catfish cultivation is primarily determined by creating a suitable environment for catfish. One way to create a suitable environment for catfish cultivation is the Recirculation Aquaculture System (RAS). In applying RAS, the presence of organic matter in the pond is controlled so the pond water can be reused. The presence of organic matter in pond water is determined mainly by the activity of bacteria in the water. These bacteria are actively involved in the pond's residual organic matter degradation process. Therefore, the total number of bacteria in the water can be one of the essential parameters in the RAS. The method widely used to calculate the abundance of bacteria is counting the number of bacteria based on bacterial culture method. However, the bacteria that can be cultured are only a small part of the bacteria in the water, so the abundance value obtained does not reflect the actual bacterial population. Therefore, it is necessary to analyze the abundance of bacteria using a method closer to the actual abundance value, such as the environmental DNA (eDNA) analysis. This study aims to analyze the abundance of bacteria in water in catfish aquaculture with RAS using the eDNA method. In addition, this study also analyzes water quality data (temperature, pH, Dissolved Oxygen). This study's results indicate that the water temperature value in catfish ponds ranges from 28.0 to 29.0 °C, the average pH value is 7.7, and the dissolved oxygen is between 5.7 - 6.2 mg/L. The water quality analysis results indicate that RAS can maintain optimum conditions in the catfish cultivation process. This study also showed that the total microbial abundance value at the beginning of cultivation was 1.68 × 107 cells/ml, and on day 30, it was 3.6 × 106 cells/ml. The dynamic of bacterial densities in this study may indicate that this system can maintain the stability of the microbial community. 
Aplikasi Tandon-Filter untuk meningkatkan Kualitas dan Kuantitas Garam dalam Teknologi Greenhouse Salt Tunnel di Pantai Selatan dan Utara Jawa Timur Amin, Abd. Aziz; Salamah, Lutfi Nimatus; Zamzami, Ilham Misbakudin Al; Susanti, Yogita Ayu Dwi; Yanuar, Adi Tiya; Kurniawan, Andi
Rekayasa Vol 17, No 1: April, 2024
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/rekayasa.v17i1.24877

Abstract

Salt is an essential commodity that concerns the livelihood of society and cannot be substituted by other commodities. East Java is the largest salt-producing center in Indonesia. The results of community salt production often still have NaCl content 95%. To achieve salt production results with NaCl quality 96%, research, and technology development are needed to eliminate impurities and improve the quality of the salt produced. One of the technologies being developed is the utilization of reservoirs and raw water filters. This research aims to analyze the application of reservoir and filter technology in improving the quality and quantity of salt production on the South and North Coasts of East Java using greenhouse salt tunnels. The results of this study indicate that the dynamics of environmental parameters and the quality of raw water on the South and North Coasts of East Java affect the results of the salt production process. The quality and quantity tests of salt with reservoir and filter application on the South Coast of East Java show NaCl quality of 98.13%, water content of 5.44%, and whiteness presentation value of salt 80.5% with production quantity of 7 kg/m². In trials on the North Coast of East Java, the salt quality was 98.25%, water content was 5.37%, and the whiteness percentage value of salt was 79%, with a salt production quantity of 7.8%. This research indicates that reservoir and filter technology can improve the quality and quantity of salt according to the characteristics of the South Coast and North Coast of East Java.
Analisis Dinamika Faktor Lingkungan Selama Proses Pengkristalan Garam di Greenhouse Salt Tunnel (Studi kasus di Pantai Utara dan Pantai Selatan, Jawat Timur, Indonesia) Amin, Abd Aziz; Yanuar, Adi Tiya; Pramudia, Zulkisam; Susanti, Yogita Ayu Dwi; Zamzami, Ilham Misbakudin AL; Salamah, Lutfi Ni’matus; Lestariadi, Riski Agung; Hakim, Lukman; Ardian, Gatot; Hanifuddin, Mokh; Kurniawan, Andi
Jurnal Penelitian Pendidikan IPA Vol 11 No 4 (2025): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i4.7131

Abstract

Salt is an essential commodity for both domestic use and industrial applications, with East Java Province in Indonesia playing a crucial role in national salt production. Traditional salt production methods, primarily based on natural evaporation, face challenges due to seasonal factors, particularly during the rainy season. This study investigates the environmental conditions affecting the salt crystallization process in the Greenhouse Salt Tunnel (GST) technology. Field experiments were conducted at two sites, Tuban Regency on the North Coast and Malang Regency on the South Coast, where key parameters such as air temperature, water temperature, humidity, wind speed, and evaporation rates were monitored three times daily. The findings showed that water temperatures within the GST ranged from 31 to 38°C on the North Coast and 26 to 38°C on the South Coast. Wind speeds varied between 1.5–2.4 m/s on the North Coast and 0.4–1.0 m/s on the South Coast, while humidity levels ranged from 54 to 80%. The GST system achieved a salt production rate of 6.4 kg/m² with NaCl content exceeding 94%, a significant improvement over traditional methods, which yield less than 70% NaCl. These results demonstrate that GST technology enhances both the quality and quantity of salt production, though design modifications are needed to address the dynamic coastal environment.
Induksi Teknologi Budikdamber (Budidaya Ikan Dalam Ember) Untuk Rumah Pangan Generasi Milenial Pramudia, Zulkisam; Moehammad, Khibar Syiar; Susanti, Yogita Ayu Dwi; Dewi, Citra Satrya Utama; Dailami , Muhammad; Gafur, Abdul
Yumary: Jurnal Pengabdian kepada Masyarakat Vol. 6 No. 2 (2025): Desember
Publisher : Penerbit Goodwood

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35912/yumary.v6i2.4769

Abstract

Purpose: The 1000 Villages Strategic Service Activity aims at community service activities with this strategic service scheme to introduce household-scale cultivation technology, namely Budikdamber, for millennial generation food houses to support increased food security in Bondowoso Regency. Methodology/approach: This service was carried out in Bondowoso Regency Maes an. These locations are at SMK Negeri 1 Maesan, and the Az Zahra Mosque Youth Secretariat, Pekingan Village. The method for implementing Strategic Service Grant activities is designed using a participatory approach, where both the activity implementer (UB) and the recipient (target partner) are proactive in carrying out service activities. Results/findings: The parameter for success is that the millennial generation has succeeded in increasing their knowledge and practice of fish cultivation through induction of Budikdamber technology, assistance with the Budikdamber system to increase analytical skills in cultivating aquaponic systems, and the process of mentoring water quality management. Conclusion: The community service program was successfully implemented by partner participants as a household-scale aquaculture solution. Its success was demonstrated through their understanding of water quality management based on CBIB principles. Limitations: The study cultivating aquaponic systems, and the process of mentoring water quality management. Contribution: This community service activity is the induction of household-scale aquaculture technology, namely Budikdamber, for millennial home-based food systems to support improved food security in Bondowoso Regency, which was successfully implemented and carried out by the partners.
Co-Authors Abd. Gafur Amin, Abd Aziz Amin, Abd. Aziz Andi Kurniawan Ardian, Gatot Dailami , Muhammad Dewi, Citra Satrya Utama Guntur Guntur Hanifuddin, Mokh Lestariadi, Riski Agung Lukman Hakim Moehammad, Khibar Syiar Okuda, Hideki Pramudia, Zulkisam Salamah, Lutfi Nimatus Salamah, Lutfi Ni’matus Wiryawan, Adam Yanuar, Adi Tiya Yuni Kilawati Zamzami, Ilham Misbakudin Al