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All Journal Asian Journal of Science, Technology, Engineering, and Art Kwaghe International Journal of Sciences and Technology Open Access DRIVERset
Hannah, Nuhu Tubasen
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Agriculture, Biological Sciences & Forestry Arts Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Engineering Environmental Science Law, Crime, Criminology & Criminal Justice Social Sciences

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Antibiofilm Activity of Silver Nanoparticles Synthesized from Seed Extract of Garcinia Kola Patricia, Okonofua Eghe; Nkene, Istifanus Haruna; David, Bando Christopher; Hannah, Nuhu Tubasen; Ngozi, Odii Josephine; Rebecca, Ayodele; Odii, Umahi Onu
Asian Journal of Science, Technology, Engineering, and Art Vol 2 No 4 (2024): Asian Journal of Science, Technology, Engineering, and Art
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstea.v2i4.3617

Abstract

Silver nanoparticles from plant extracts are novel compounds with potential antimicrobial properties. Studies on antibiofilm activity of Ag-NPs synthesized from seed extracts of Garcinnia kola (G. kola) were carried out. Garcinnia kola seed were obtained from Keffi market, Nigeria. Green synthesis of Ag-NPs from the seed was carried using 2.0mm silver-nitrate by use of standard method. The Ag-NPs synthesized from the seed were characterized using former transmission infrared (FITR) spectroscopy and scanning election microscope. The antimicrobial activity of the Ag-NPs against Klebsiella pneumonia (Kp) isolates were carried out using agar dilution method. The biofilm formation by the isolates as well as the inhibition and dissolution by Ag-NPs were eval__uated using microplate method. The functional groups detected in the Ag-NPs were N-H, C-O, N-O, and CΞC with peaks 906.5cm-1,1282.2cm-2, 13344cm-1, 1550.6cm-1 and 217.1cm-1 respectively. The size of the particles ranges from 179-296nm. The minimum inhibiting concentration (MICs) of the particles and meropenem against the isolates were 250µg/l and 4.0µg/l. The functional inhibiting concentrates of the particles were 1.0. The optical clarity of biofilm formed by the isolates was 2.073 and 2.049. the percentage biofilm inhibiting effects of the particles was highest apart. KpC (K. Pneumoniae ATCC BAA 1075) with percentage inhibit ranges from 27.28-21.67% at 80-12.5% of the MICs. The percentage inhibiting effect of Ag-NPs in with meropenem was highest at MICs but low in MIC 12.5 with percentage inhibition 28.26% and 27.18%. The Ag-NPs alone and antibacterial activity and biofilm inhibiting effect while Ag-NPs in with meropenem had effect but against isolate but with potential antibiofilm activity.
Evaluation of the Effect of Pre-Germination Treatment on Delonix Regia Seeds Ogu, Odiba Emmanuel; Joshua, Efi Uwhe; Haruna, David Ephraim; Samaila, Danjuma; Hannah, Nuhu Tubasen; Patricia, Okonofua Eghe; Rebecca, Ayodele; David, Bando Christopher
Kwaghe International Journal of Sciences and Technology Vol 1 No 1 (2024): Kwaghe International Journal of Sciences and Technology
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/kijst.v1i1.3292

Abstract

Delonix regia (Flamboyant) is a multipurpose tree species indigenous to semi-arid regions of Sub-Saharan Africa. It is being exploited to local extinction due to high dependence for fuel wood and other uses. The present study explored different pre-treatment methods for enhancing seed germination and growth of Delonix regia (Flamboyant) in Taraba State, Northern Nigeria. The experiment employed a 4 × 4 factorial design with seeds subjected to four pre-treatments (50% sulphuric acid concentration, 98% sulphuric acid concentration, cold water and hot water) at four pre-treatment time durations. Number of germination and plant height varied significantly between pre-treatments with cold water treatment recording the highest plant height (3.5cm) and the 98% sulphuric acid concentration recording the least (1.3cm). Germination rate had a moderate positive relationship with plant height. The study recommends seed immersion in cold water for 48 h as the most efficient pre-treatment for Delonix regia (Flamboyant).
Evaluation of the Effect of Pre-Germination Treatment on Delonix Regia Seeds Ogu, Odiba Emmanuel; Joshua, Efi Uwhe; Haruna, David Ephraim; Samaila, Danjuma; Hannah, Nuhu Tubasen; Patricia, Okonofua Eghe; Rebecca, Ayodele; David, Bando Christopher
Kwaghe International Journal of Sciences and Technology Vol 1 No 1 (2024): Kwaghe International Journal of Sciences and Technology
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/kijst.v1i1.3292

Abstract

Delonix regia (Flamboyant) is a multipurpose tree species indigenous to semi-arid regions of Sub-Saharan Africa. It is being exploited to local extinction due to high dependence for fuel wood and other uses. The present study explored different pre-treatment methods for enhancing seed germination and growth of Delonix regia (Flamboyant) in Taraba State, Northern Nigeria. The experiment employed a 4 × 4 factorial design with seeds subjected to four pre-treatments (50% sulphuric acid concentration, 98% sulphuric acid concentration, cold water and hot water) at four pre-treatment time durations. Number of germination and plant height varied significantly between pre-treatments with cold water treatment recording the highest plant height (3.5cm) and the 98% sulphuric acid concentration recording the least (1.3cm). Germination rate had a moderate positive relationship with plant height. The study recommends seed immersion in cold water for 48 h as the most efficient pre-treatment for Delonix regia (Flamboyant).
Co-Authors David, Bando Christopher Haruna, David Ephraim Joshua, Efi Uwhe Ngozi, Odii Josephine Nkene, Istifanus Haruna Odii, Umahi Onu Ogu, Odiba Emmanuel Patricia, Okonofua Eghe Rebecca, Ayodele Samaila, Danjuma