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All Journal Indonesian Journal of Chemistry
Yuswan, Mohd Hafis
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Chemical Engineering, Chemistry & Bioengineering Chemistry

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Evaluation of Extraction Buffers for Protein Identification in Fish Paste with Chicken Blood Plasma (CBP) Spikes: A Preliminary Mass Spectrometry Study Azmi, Nurhazirah; Abidin, Siti Aimi Sarah Zainal; Zakaria, Nurkhurul Ain; Shukor, Mohd Syarafuddin Abdul; Karsani, Saiful Anuar; Fatt, Low Kim; Yuswan, Mohd Hafis
Indonesian Journal of Chemistry Vol 25, No 2 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.100755

Abstract

Blood plasma is commonly used as a protease inhibitor in surimi production to improve product quality and stability during processing. However, its use in food is prohibited in Islam and classified as najs al-mutawasitah, necessitating the detection of blood plasma adulteration to uphold halal standards and food safety. This study compared the effectiveness of different extraction buffers—ultrapure water, 0.05 M Tris-HCl, 0.05 M Tris-Urea, and ultrapure water followed by acetone precipitation—on protein and peptide yield from chicken blood plasma (CBP) using liquid chromatography–quadrupole linear ion trap mass spectrometry (LC-QTRAP-MS). Ultrapure water and acetone precipitation yielded the highest protein content, prompting further proteome profiling of CBP, fish paste, and surimi spiked with CBP (0.5, 1, and 1.5%) via liquid chromatography–quadrupole time-of-flight mass spectrometry (LC–QTOF-MS). Apolipoprotein AI (Apo AI) and fibrinogens emerged as key proteins in CBP. Apo AI was detected in all spiked surimi samples, demonstrating its potential as a marker for blood plasma contamination. The proposed method enhances extraction and detection protocols, using mass spectrometry to provide a reliable tool for addressing halal compliance and mitigating food safety risks associated with blood-derived adulterants in surimi products.
Co-Authors Abidin, Siti Aimi Sarah Zainal Azmi, Nurhazirah Fatt, Low Kim Karsani, Saiful Anuar Shukor, Mohd Syarafuddin Abdul Zakaria, Nurkhurul Ain