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All Journal Indonesian Journal of Chemistry Makara Journal of Health Research
Karsani, Saiful Anuar
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Chemical Engineering, Chemistry & Bioengineering Chemistry

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Comparative Analysis of Proteomics Biomarkers Associated with Residual Ridge Resorption Induced by Denture Wear Ahmad, Rohana; Mohamad Napi, Ainin Sofia; Lim, Tong Wah; Tan, Su Keng; Karsani, Saiful Anuar; Mazlan, Musalmah; Teh, Lay Kek; Morgano, Steven M; Baba, Nadim Z
Makara Journal of Health Research Vol. 25, No. 2
Publisher : UI Scholars Hub

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Abstract

Background: The biochemical bone turnover markers for residual ridge resorption (RRR) are unclear. Therefore, the present study aimed to determine the biochemical bone turnover markers associated with RRR by comparing proteomics between the compressed mucosa of denture wearers and the non-compressed mucosa of non-denture wearers. Methods: The mucosal specimens of 11 complete-denture wearers were obtained from the alveolar ridge during surgical implant exposure for implant-retained overdentures. All denture wearers had been edentulous and worn dentures for at least 5 years. The tissues of 11 non-denture wearers were taken from the ridge during minor preprosthetic surgery. The mucosal proteins were extracted, purified, precipitated, and subsequently separated by two-dimensional gel electrophoresis for comparative proteomics. Differentially expressed proteins between the groups were analyzed by ANOVA using Progenesis SameSpots software. Results: Comparative proteomics analysis showed significant upregulation of 78 kDa glucose-regulated protein (GRP78; +2.2 fold, p = 0.015) and lumican (+1.8 fold, p = 0.005), as well as significant downregulation of heat shock protein 27 (HSP27; −1.9 fold, p = 0.029) in the denture group. Conclusions: Differential expression of the biochemical bone turnover markers of GRP78, lumican, and HSP27 may occur as a result of denture pressure on the mucosa. These markers may play important roles in RRR.
Evaluation of Extraction Buffers for Protein Identification in Fish Paste with Chicken Blood Plasma (CBP) Spikes: A Preliminary Mass Spectrometry Study Azmi, Nurhazirah; Abidin, Siti Aimi Sarah Zainal; Zakaria, Nurkhurul Ain; Shukor, Mohd Syarafuddin Abdul; Karsani, Saiful Anuar; Fatt, Low Kim; Yuswan, Mohd Hafis
Indonesian Journal of Chemistry Vol 25, No 2 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.100755

Abstract

Blood plasma is commonly used as a protease inhibitor in surimi production to improve product quality and stability during processing. However, its use in food is prohibited in Islam and classified as najs al-mutawasitah, necessitating the detection of blood plasma adulteration to uphold halal standards and food safety. This study compared the effectiveness of different extraction buffers—ultrapure water, 0.05 M Tris-HCl, 0.05 M Tris-Urea, and ultrapure water followed by acetone precipitation—on protein and peptide yield from chicken blood plasma (CBP) using liquid chromatography–quadrupole linear ion trap mass spectrometry (LC-QTRAP-MS). Ultrapure water and acetone precipitation yielded the highest protein content, prompting further proteome profiling of CBP, fish paste, and surimi spiked with CBP (0.5, 1, and 1.5%) via liquid chromatography–quadrupole time-of-flight mass spectrometry (LC–QTOF-MS). Apolipoprotein AI (Apo AI) and fibrinogens emerged as key proteins in CBP. Apo AI was detected in all spiked surimi samples, demonstrating its potential as a marker for blood plasma contamination. The proposed method enhances extraction and detection protocols, using mass spectrometry to provide a reliable tool for addressing halal compliance and mitigating food safety risks associated with blood-derived adulterants in surimi products.
Co-Authors Abidin, Siti Aimi Sarah Zainal Ahmad, Rohana Azmi, Nurhazirah Baba, Nadim Z Fatt, Low Kim Lim, Tong Wah Mazlan, Musalmah Mohamad Napi, Ainin Sofia Morgano, Steven M Shukor, Mohd Syarafuddin Abdul Tan, Su Keng Teh, Lay Kek Yuswan, Mohd Hafis Zakaria, Nurkhurul Ain