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Sahidin, Idin

Universitas Padjadjaran, Indonesia

Author-ID : 839167

Medicine & Pharmacology

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Antioxidant Activity of Ethanol Extract of Polygonum pulchrum Blume Sadino, Asman; Sahidin, Idin; Wahyuni, Wahyuni
Pharmacology and Clinical Pharmacy Research Vol 1, No 2
Publisher : Universitas Padjadjaran, Indonesia

The use of antioxidants from natural resources has attracted increasing interest. One of theplant that was empirically used as an antioxidant dietary supplement was Polygonum pulchrumBlume (P. pulchrum Blume). This study aimed to investigate antioxidant activityof roots, stems, leaves and flowers ethanol extract of P. pulchrum Blume. The extract wasobtained by maceration method using ethanol solvent. Antioxidant activity was determinedwith 1,1-diphenyl-picrylhydrazyl (DPPH) method. We found that ethanol extracts of P. pulchrumBlume roots and stems had strong antioxidant activity with IC50 values of 25.2 mg/land 43.26 mg/l, respectively. Ethanol extract of flowers had the weakest antioxidant activitywith IC50 value of 202.96 mg/l. Vitamin C had very strong antioxidant activity with IC50value of 3.97 mg/l. In conclusion, our study revealed that ethanol extract of Polygonumpulchrum Blume roots and stems had strong antioxidant activity. Therefore, P. pulchrumBlume might be potential as an excellent source for natural antioxidant agents for medicalapplication.Keywords: Polygonum pulchrum Blume, DPPH, antioxidant, maceration, ethanol

Antibacterial Activity of Polygonum pulchrum Blume Ethanol Extract on Staphylococcus aureus and Escherichia coli Sadino, Asman; Sahidin, Idin; Wahyuni, Wahyuni
Pharmacology and Clinical Pharmacy Research Vol 3, No 2 (2018)
Publisher : Universitas Padjadjaran, Indonesia

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The emergence of resistant bacteria strain has become a global health concern. It encourages the exploration of potential antibacterial agents, particularly from natural sources. The aim of this study was to investigate the antibacterial activity of ethanol extract of root, stems, leaves, and flowers of Polygonum pulchrum Blume against Staphylococcus aureus and Escherichia coli, through disc diffusion method using cup-plate method. Inhibition zone against S. aureus from roots, stems, leaves, and flowers ethanol extract were 3.5 mm, 2.5 mm, 2.25 mm, and 2.62 mm, respectively, while the inhibition zone against E. coli were 2.25 mm, 2.12 mm, 1.62 mm, and 1.75 mm, respectively. In conclusion, ethanol extract of root, stem, leaves, and flower of P. pulchrum Bl possessed weak antibacterial activity against S. aureus and E. coli.Keywords: P. pulchrum Bl, antibacterial, E. coli, S. aureus, cup-plate technique

Acute Toxicity of Ethanol Extract of Polygonum pulchrum Blume using Brine Shrimp Lethality Test Method Sadino, Asman; Sahidin, Idin; Wahyuni, Wahyuni
Pharmacology and Clinical Pharmacy Research Vol 2, No 2
Publisher : Universitas Padjadjaran, Indonesia

The potential toxicity effect presents in the medicinal plants is important to be identified forthe safety assurance. Accute toxicity study is an initial step in the drug safety assurance test.Thus, the aim of this study is to identify the potential acute toxicity of roots, stems, leaves,and flowers ethanol extracts of bamboo bamboo plants (Polygonum pulchrum Blume). Thisstudy used brine shrimp lethality test (BSLT) method. The total number of larvae used ineach concentrations in three times replications was 330 larvae. Each group was given consecutivelyroots, stems, leaves and flowers ethanol extracts of P. pulchrum Blume plants withvariation concentrations 12.5 Î¼g/ml, 25 Î¼g/ml, 50 Î¼g/ml, 100 Î¼g/ml, 125 Î¼g/ml, 250 Î¼g/ml, 500 Î¼g/ml, 1000 Î¼g/ml, 2000 Î¼g/ml, and 4000 Î¼g/ml. The number of dead larvae after24 hours treatment were calculated to obtain the mortality percentages and to determine theLC50 value, which were determined by probit analysis using MiniTab application version17.1.0. Ethanol extract of P. pulchrum roots and stems resulted LC50 values of 933.08 Î¼g/mland 919.58 Î¼g/ml, respectively. While the LC50 values of leaves and flowers extracts were2207.06 Î¼g/ml and 1081.90 Î¼g/ml, respectively. In conclusion, P. pulchrum Blume roots andstems were classified as toxic according to BLST method, while leaves and flowers were not.Keywords: acute toxicity, brine shrimp lethality test, Polygonum pulchrum Blume

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