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All Journal HAYATI Journal of Biosciences Indonesian Journal of Mollusk
Lumban Gaol, Josua Gabriel
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science

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Screening for Antibacterial and Antifungal Activity in Fungi Associated with Molluscs Lumban Gaol, Josua Gabriel; Pringgenies, Delianis; Ari Setyati , Wilis
Jurnal Moluska Indonesia Vol. 9 No. 1 (2025): April 2025
Publisher : Masyarakat Moluska Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54115/jmi.v9i1.114

Abstract

Molluscs are invertebrate organisms that produce secondary metabolites to adapt to their environment. These secondary metabolites are also found in fungi that live in symbiosis with molluscs. Research has shown that these symbiotic fungi produce various secondary metabolites with antibacterial and antifungal properties. This study aims to explore the potential of fungi associated with molluscs in developing bioprospecting strategies against pathogenic bacteria and fungi. The antibacterial and antifungal screening tests were conducted against the pathogenic bacteria Escherichia coli and Staphylococcus aureus, and the pathogenic fungi Candida albicans and Trichoderma harzianum, over a period of 36 hours. The results indicated that 12 isolates of mollusk-associated fungi exhibited antibacterial activity against the tested bacterial pathogens. Additionally, 4 of these 12 isolates also demonstrated antifungal activity. The antibacterial and antifungal activities were identified by the clear zones formed in the test medium. The screening tests revealed that fungi associated with molluscs possess antibacterial and antifungal activities against several tested pathogenic bacteria and fungi. Keywords: Pathogenic Bacteria; Association Fungi; Pathogenic Fungi; Molluscs
Seaweed Diversity and Bioactive Compounds in Panjang Islands, Central Java, Indonesia Setyati, Wilis Ari; Pramesti, Rini; Putri, Angela Salsalina; Risandhi, Danendra Aquila Azfa; Firdaus, Syifa Shafira; Lumban Gaol, Josua Gabriel
HAYATI Journal of Biosciences Vol. 33 No. 3 (2026): May 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.3.669-680

Abstract

The biodiversity of seaweed encompasses a wide array of potential bioactive compounds applicable to various industries, particularly pharmaceuticals. This study aimed to collect seaweed diversity data from Panjang Island, Jepara, Central Java, Indonesia, and to identify the bioactive compounds and biological activity of each seaweed species for preliminary screening. Random sampling was used to collect the sample. Qualitative identification of bioactive compounds was performed using the maceration method for extraction, phytochemical screening tests, and pigment identification based on Rf values on TLC. Antibacterial screening tests were performed against Gram-positive (S. aureus) and Gram-negative (E. coli) bacteria, followed by testing against pathogenic fungi (C. albicans) using the disk diffusion method, and an antioxidant test using the DPPH method. The results showed that six species from three phyla (Chlorophyta, Phaeophyta, and Rhodophyta) exhibited distinct morphological characteristics, and the types of bioactive compounds produced by each species differed. The biological activity test results showed a low inhibitory activity. Antibacterial and antifungal biological activities were at the value of (<5 mm), and antioxidant biological activity was (>750 ppm). However, the active compounds and pigments with potential antibacterial, antifungal, and antioxidant properties can be optimized in various fields of bioindustry in the future.
Co-Authors Ari Setyati , Wilis Delianis Pringgenies Firdaus, Syifa Shafira Putri, Angela Salsalina Rini Pramesti Risandhi, Danendra Aquila Azfa Wilis Ari Setyati