Rafiq, Faizal
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The Detection and Analysis of Factors Associated with the Incidence of Lumpy Skin Disease in Cattle Transhipped at Merak Port, Indonesia Rafiq, Faizal; Murtini, Sri; Mujiatun, Mujiatun; Dewi, Arum Kusnila; Adiningsih, Melani Wahyu
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.26-39

Abstract

Lumpy Skin Disease (LSD) is caused by the Lumpy Skin Disease Virus (LSDV) that affects cattle and buffalo. The symptoms include the development of lumps or nodules on the skin of infected animals. Therefore, this study aimed to detect the presence of LSD and determine factors associated with the incidence in cattle transhipped through Merak Port. Samples were collected in the form of oral and nasal swabs, then tested with quantitative real-time PCR (qPCR). The results showed that there were two positive LSD samples confirmed by molecular testing using qPCR. The positive cattle did not show clinical signs or were suspected to be sub-clinically infected, while the Ct values obtained were 27.71 and 28.88. The use of molecular methods with qPCR showed relatively good results for the detection of LSD. Cattle that did not show clinical signs were detected as positive by the test. This is because qPCR can detect viruses more quickly and accurately, even at very low viral load levels. Factors associated with the incidence of LSD in the Merak Port (p < 0.05) were farm origin and biosecurity measures, knowledge of livestock handlers, vaccination status, and disinfection practices.
Detection of Lumpy Skin Disease Virus in Transhipped Through Merak Port and The Impact of its Spread Rafiq, Faizal; Murtini, Sri; -, Mujiatun; -, Helmi; Raditya, Mochammad Nova
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.95129

Abstract

Research to detect the presence of Lumpy Skin Disease Virus (LSDV) was carried out in the work area of the Banten Animal, Fish and Plant Quarantine Center (BBKHIT). Sampling for LSDV detection was taken from cattle that were being transported both in and out of Merak Port. The aim of this research is to detect the presence of LSDV both clinically and in laboratory examinations at Merak Port and estimate the impact of its spread. 152 mouth and nose swab samples were taken, then a quantitative real time PCR (qPCR) test was carried out. The results of the study showed that there were no clinical symptoms observed in the trafficked cattle, however there were 2 positive samples for LSD by molecular testing using qPCR. These results can be said to be subclinically infected cattle. Positive samples came from cattle trafficked from Sumbawa Regency and Central Lampung Regency with respective Ct values of 27.71 and 28.88. Estimates of the impact of the spread of LSDV were carried out based on the categories of breeding cattle and beef cattle. The impact of the spread of LSDV is estimated to be more dangerous and will occur in breeding cattle because of their long lifespan during the rearing process. This means that the LSD virus can continue to spread to other cows rather than cows that are immediately slaughtered.