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Anjas, Anjas Wilapangga
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Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

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Skrining Fitokimia dan Uji Antibakteri Ekstrak Daun Jambu Kristal (Psidium guajava cv. Kristal L.) Nicolas Yonathan; Anjas, Anjas Wilapangga; Sri Royani
The Journal Of Pharmacy Vol. 1 No. 2 (2024): Journal Of Pharmacy UMRI
Publisher : LPPM, Universitas Muhammadiyah Riau

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Abstract

The crystal guava plant or (Psidium guajava cv. Kristal L) grows abundantly in tropical regions, especially in Kemangkon, Purbalingga, Indonesia. This plant is known for its health benefits, one of which is as an antibacterial agent due to its secondary metabolite content. This research is an experimental study that examines the secondary metabolites and antibacterial properties of crystal guava leaf extract from Kemangkon, Purbalingga. The method used is maceration extraction with 96% ethanol solvent, phytochemical screening including saponins, tannins, flavonoids, and alkaloids, and antibacterial testing using the disc diffusion method with concentrated extracts at 50 ppm and 150 ppm tested against Staphylococcus aureus, with chloramphenicol as a positive control. Phytochemical results show that crystal guava leaves contain alkaloids, flavonoids, saponins, and tannins. Antibacterial tests prove that ethanol extract of crystal guava leaves effectively inhibits bacteria, especially at concentrations of 50 ppm and 150 ppm. At 50 ppm, the inhibition zone within 24 hours reaches 1.3 cm and expands to 1.5 cm after 48 hours. At 150 ppm, the inhibition zone reaches 1.7 cm within 24 hours and remains the same after 48 hours.
Skrining Fitokimia Ekstrak Etanol 96% Bunga Telang (Clitoria ternatea L.) Dan Uji Kemampuan Sebagai Antibakteri Niken Hataningtyas; Anjas, Anjas Wilapangga; Sri Royani
The Journal Of Pharmacy Vol. 1 No. 2 (2024): Journal Of Pharmacy UMRI
Publisher : LPPM, Universitas Muhammadiyah Riau

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Abstract

Indonesia secara geografis memiliki letak yang strategis untuk ditumbuhi tanaman herbal seperti bunga telang. Bunga telang dengan nama ilmiah Clitoria ternatea L. tergolong ke dalam keluarga Fabaceae yang merupakan salah satu jenis tanaman yang hidup dengan baik di Indonesia. Bunga telang merupakan salah satu tanaman yang telah lama digunakan dalam pengobatan dan telah terbukti memiliki aktivitas sebagai antibakteri. Salah satu metabolit sekunder yang berperan sebagai antibakteri yaitu flavonoid. Oleh karena itu dilakukan penelitian terkait bunga telang sebagai antibakteri. Berdasarkan hasil skrining fitokimia ekstrak etanol 96% bunga telang yang telah dilakukan, diketahui bahwa bunga telang positif mengandung alkaloid, flavonoid, saponin dan tanin. Ekstrak bunga telang diperoleh dengan metode maserasi dengan menggunakan pelarut etanol 96%. Pada proses maserasi yang telah dilakukan diperoleh hasil rendemen sebesar 22,5%. Pada pengujian antibakteri menunjukkan bahwa ekstrak etanol 96% bunga telang dapat menghambat bakteri Strapylococcus aureus. Pengujian antibakteri dilakukan menggunakan metode difusi cakram, dimana cakram kosong direndam pada ekstrak etanol 96% bunga telang dengan berbagai konsentrasi. Ekstrak etanol 96% bunga telang untuk pengujian antibakteri dibuat dengan konsentrasi 50 ppm dan 100 ppm. Sedangkan kontrol positif yang digunakan yaitu antibiotik chloramphenicol. Pada penelitian ini ekstrak etanol 96% bunga telang dengan konsentrasi 50 ppm memiliki daya hambat terhadap bakteri Straphylococcus aureus sebesar 1,04 mm dan pada konsentrasi 100 ppm memiliki daya hambat sebesar 1,72 mm. Dengan demikian ekstrak etanol 96% bunga telang konsentrasi 100 ppm memiliki daya hambat terhadap bakteri Strapylococcus aureus lebih besar dibandingkan konsentrasi 50 ppm.
Green Synthesis Nanopartikel Perak (NPAg) Menggunakan Ekstrak Etanol 96% Daun Kelor (Moringa oleifera) Sebagai Antibakteri Viderika Haini Nurfadia; Anjas, Anjas Wilapangga; Sri Royani
The Journal Of Pharmacy Vol. 1 No. 2 (2024): Journal Of Pharmacy UMRI
Publisher : LPPM, Universitas Muhammadiyah Riau

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Abstract

Indonesia has various types of plants that can be used as medicine, one of which is moringa leaves (moringa oleifera). To utilize Indonesia's natural wealth, a method was developed in a drug delivery system that uses the principle of nanoparticles by utilizing medicinal plants as reductants. This study aims to prove that moringa leaf extract functions as a bioreductor and the synthesized silver nanoparticles have antibacterial potential. The nanoparticle synthesis method used in this study is the green synthesis method. Moringa leaves (Moringa oleifera) were extracted using the maceration method, then the 100 ppm concentration extract was used to synthesize silver nitrate (AgNO3). The silver nanoparticle solution was characterized using UV-Vis spectrophotometry and tested for antibacterial activity. An extract yield of 40.85% was obtained. The synthesis process of silver nanoparticles solution is brownish yellow in 6.56 seconds, has a wavelength of 400 nm with an absorbance value of 0.966. Antibacterial activity is indicated by the formation of a clear zone around the disc paper, silver nanoparticles showed an inhibition zone of 1.44 cm on petri dish 1 and 1.25 cm on petri dish 2. Based on the research that has been done, flavonoids in Moringa leaves are able to act as bioreductors in the synthesis of silver nanoparticles. In addition, the silver nanoparticles obtained have potential as antibacterial agents.
Co-Authors Nicolas Yonathan Niken Hataningtyas sri royani Viderika Haini Nurfadia