Farhan, Rana Salim
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L-Carnitine Treatment of Adult Rats With Busulfan-Induced Oligospermia Owain, Fahad Saber; Mejbel, Elham Ahmed; Farhan, Rana Salim; Saleh , Eman Naji
Indonesian Journal on Health Science and Medicine Vol. 1 No. 2 (2024): October
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/ijhsm.v1i1.12

Abstract

Abstract. The purpose of this research was to examine the effects of L-carnitine treatment on adult rats that had busulfan-induced oligospermia. In our experiment, we used twenty-one adult male rats, each weighing around 200 g. Three groups of rats were used for the experiments: The first set of subjects, called the control group, had DMSO administered intraperitoneally and 1 mL of pure water gavaged orally once daily. The second group received 20 mg/kg of busulfan (Bus) administered IP once daily along with a daily oral gavage of 1 ml of distilled water. The third set of subjects received a single I.P. of busulfan along with L-carnitine (L-car) and busulfan administered orally. One day after busulfan injection, L-carnitine were administered for a duration of 28 days at 350 mg/kg.bw. After dissecting, the epididymis and testis were meticulously removed and weighed, along with any excess fat and blood. The spermatozoa were taken from the removed caudal epididymis and incubated in PBS medium containing at 37C for 5 minutes, this used for counting and checking abnormalities. MDA and GSH were also estimated in testes tissues by using HPLC. The administration of BU resulted in a substantially testes weight, and relative testes weight (p <.05) as compared to the control group. testes weight, and relative testes weight were all markedly improved with L-carnitine administration as compared to G2. after administering BU, sperm count and motility were significantly lower (p <.05) compared to G1. There was a significant improvement in motility, sperm count, and abnormalities with L-carnitine administration compared to G2. after BU was administered, MDA were considerably higher and GSH significantly lower (p <.05) as compared to G1. The results of measuring oxidative stress indicators were dramatically improved with L-carnitine administration when contrasted with G2. In conclusion, Busulfan may have a negative impact on male fertility if taken alone, but when combined with L-carnitine, the drug's cytotoxic effects can be mitigated, protecting both testis and semen. Highlights: L-carnitine Treatment: Significantly improved testis weight and sperm quality in busulfan-treated rats. Oxidative Stress Reduction: L-carnitine mitigated increased MDA levels and restored GSH in testicular tissue. Fertility Protection: Co-administration of L-carnitine reduced the cytotoxic effects of busulfan on male fertility. Keywords: L-carnitine, busulfan, oligospermia, male fertility, oxidative stress
Plesiomonas Shigelloides: Unraveling the Mysteries of a Neglected Enteric Pathogen: Plesiomonas Shigelloides: Mengungkap Misteri Patogen Enterik yang Terabaikan Altaee, Zainab Agab; Farhan, Rana Salim; Jameel, Nabaa Qais; Saleh, Eman Naji
Indonesian Journal on Health Science and Medicine Vol. 1 No. 2 (2024): October
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/ijhsm.v1i2.30

Abstract

The oxidase-positive, motile, Gram-negative bacteria Plesiomonas shigelloides is found all across the natural world. Additionally, it is a major pathogen that mostly causes disorders of the intestines in humans. Most of these illnesses are characterised by diarrhoea, which may be watery, invasive, or chronic in nature. There have been reports of intestinal illnesses caused by Plesiomonas that were spread by food and water. There are a number of extraintestinal diseases caused by P. shigelloides, the most prevalent of which are sepsis and meningitis, both of which are associated with significant fatality rates. Phylogenetically closely related to other Enterobacteriaceae species, P. shigelloides differs biochemically from them. A single biovar, with over a hundred serovars documented. Some have proposed P. shigelloides as a "natural" vaccination against shigellosis since it is thermo-, alkali-, acido-, and halotolerant. There are some intestinal media that are known to limit the development of Plesiomonas, and in the lab, it looks barely there on the surface of many agar plates. The antibiotic sensitivity patterns of Plesiomonas are somewhat peculiar, and the amount of the inoculum determines how susceptible the bacteria are to certain drugs. One distinguishing feature of high bacterial densities in the presence of certain β-lactam antibiotics is the development of significant cell filamentation Highlights: Pathogen: Causes diarrheal diseases, sepsis, and meningitis in humans. Characteristics: Gram-negative, motile, tolerates heat, alkali, acid, and salt. Antibiotics: Susceptibility varies; β-lactams cause cell filamentation in high densities. Keywords: Plesiomonas shigelloides, bacteria, diarrhea