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All Journal Jurnal Ilmiah Kedokteran Wijaya Kusuma
Teresa Lilliana Wargasetya
Department of Pathology, Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

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Modulated Hepatic Expression of NF-kB and CCN1 Genes in a Breast Cancer Mouse Model Induced by DMBA and High-Fat Diet Demes Chornelia Martantiningtyas; Raden Ghita Sariwidyantry; Hana Ratnawati; Teresa Lilliana Wargasetya; Ardo Sanjaya
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 14, No 1 (2025): EDISI MARET 2025
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jikw.v14i1.4002

Abstract

Background: The study investigates the combined effects of 7,12-dimethylbenz[a]anthracene (DMBA) and a high-fat diet (HFD) on hepatic gene expression in a breast cancer mouse model. Both DMBA and HFD are associated with oxidative stress, inflammation, and fibrosis, which may contribute to cancer progression.Methods: Female C57BL/6 mice were divided into three groups: standard diet (ND), ND + DMBA, and HFD + DMBA. Mice in the ND + DMBA and HFD + DMBA groups received DMBA injections weekly for six weeks. The HFD + DMBA group was also fed a high-fat diet for four weeks prior to DMBA administration. After a 12-week treatment period, liver tissues were collected and analyzed for NF-κB and CCN1/CYR61 gene expression using semi-quantitative RT-PCR.Results: The study revealed significant upregulation of NF-κB and CCN1/CYR61 expression in the ND + DMBA and HFD + DMBA groups compared to the control group. Notably, the HFD + DMBA group exhibited the highest expression levels of these genes, suggesting that the combination of HFD and DMBA synergistically exacerbates hepatic inflammation and fibrosis.Conclusion: The findings indicate that a high-fat diet in combination with carcinogen exposure can significantly enhance hepatic gene expression related to inflammation and fibrosis, highlighting the importance of dietary management in cancer prevention and treatment. 
Co-Authors Ardo Sanjaya Demes Chornelia Martantiningtyas Hana Ratnawati Raden Ghita Sariwidyantry