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Elisabet Sa Wulo
Universitas Negeri Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Identification of Phenolic Compounds Content in Tinospora crispa Stem Decoction by FTIR and UV-Visible Spectrophotometry Elisabet Sa Wulo; Yunike Kurnia Unda; Suparno
Jurnal Penelitian Pendidikan IPA Vol 11 No 5 (2025): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i5.10069

Abstract

Phenolics are compounds that have a hydroxyl (OH) group attached to an aromatic ring, and are known for various health benefits such as antioxidant, antimicrobial, and anti-inflammatory properties. This study aims to measure the total phenolic content and characterize the functional groups in Tinospora crispa stem extract using a decoction method, as well as evaluate its potential as a source of bioactive compounds. Extraction was performed by boiling dried Tinospora crispa stems in water. Total phenolic content was quantified using UV-Vis spectrophotometry (λ = 765 nm) via the Folin-Ciocalteu reaction, with a gallic acid calibration curve (R² = 0.998). Functional group analysis was conducted using FTIR (4000–400 cm⁻¹). The extract showed high phenolic content (384.909 mg GAE/g), supported by the identification of characteristic functional groups such as O-H (3255.55 cm⁻¹), C=C (2121.59 cm⁻¹), and C-O (1261.45 cm⁻¹) in the FTIR spectrum. These groups are associated with the presence of bioactive compounds such as phenolics. Therefore, Tinospora crispa has potential as a source of phenolic compounds, one of which can be obtained using the decoction method.
Co-Authors Suparno Suparno Yunike Kurnia Unda