Jurnal Penelitian Pendidikan IPA (JPPIPA)
Vol 11 No 5 (2025): May

Identification of Phenolic Compounds Content in Tinospora crispa Stem Decoction by FTIR and UV-Visible Spectrophotometry

Wulo, Elisabet Sa (Unknown)
Unda, Yunike Kurnia (Unknown)
Suparno (Unknown)



Article Info

Publish Date
25 May 2025

Abstract

Phenolics are compounds that have a hydroxyl (OH) group attached to an aromatic ring, and are known for various health benefits such as antioxidant, antimicrobial, and anti-inflammatory properties. This study aims to measure the total phenolic content and characterize the functional groups in Tinospora crispa stem extract using a decoction method, as well as evaluate its potential as a source of bioactive compounds. Extraction was performed by boiling dried Tinospora crispa stems in water. Total phenolic content was quantified using UV-Vis spectrophotometry (λ = 765 nm) via the Folin-Ciocalteu reaction, with a gallic acid calibration curve (R² = 0.998). Functional group analysis was conducted using FTIR (4000–400 cm⁻¹). The extract showed high phenolic content (384.909 mg GAE/g), supported by the identification of characteristic functional groups such as O-H (3255.55 cm⁻¹), C=C (2121.59 cm⁻¹), and C-O (1261.45 cm⁻¹) in the FTIR spectrum. These groups are associated with the presence of bioactive compounds such as phenolics. Therefore, Tinospora crispa has potential as a source of phenolic compounds, one of which can be obtained using the decoction method.

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Journal Info

Abbrev

jppipa

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

Description

Science Educational Research Journal is international open access, published by Science Master Program of Science Education Graduate Program University of Mataram, contains scientific articles both in the form of research results and literature review that includes science, technology and teaching ...