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Peran Ekspresi Gen Pr-1 (Pathogenesis Related Proteins-1) dalam Resistensi Tanaman dan Implikasinya pada Bioteknologi Pertanian Nia Febrianti; Dian Dwi Lestari; Yusminah Hala
Jurnal Biosilampari : Jurnal Biologi Vol 7 No 2 (2025): Biosilampari: Jurnal Biologi
Publisher : Universitas PGRI Silampari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62112/biosilampari.v7i2.199

Abstract

The pr-1 gene is a gene that encodes the PR-1 (Pathogenesis-Related 1) protein which plays a role in the plant defense response to pathogen infection. Although the role of the pr-1 gene as a defense protein has been known, the specific mechanism of how pr-1 responds to various types of pathogens in various plants is still poorly studied in various studies. Therefore, an in-depth analysis is needed regarding the role of the pr-1 gene in plant resistance. This study aims to determine the role of pr-1 (Pathogenesis Associated Proteins-1) gene expression on plant resistance and its implications for agricultural biotechnology. The research method used is a literature study with a qualitative approach. The results of the literature findings were then analyzed descriptively to determine the role of the pr-1 gene in the plant defense response. The findings show that the main role of the pr-1 gene is as a plant defense system against biotic and abiotic environmental threats. The plant resistance mechanism carried out by the PR-1 Protein will be active if there is PAMP/MAMP detection received by the plant cell surface receptor PRR (Pattern Recognition Receptors) to carry out the function of gene expression in plant organs.
The Effect of Project-Based Learning (PjBL) with STEM Approach on Students’ Metacognitive Awareness, Conceptual Understanding, and Biology Problem-Solving Ability Nia Febrianti; Firdaus Daud; Yusminah Hala; Muhiddin Palennari; Nurhayati B
Jurnal Penelitian Pendidikan IPA Vol 12 No 4 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i4.15006

Abstract

This study aims to analyze effect of the Project-Based Learning model based on the STEM approach on metacognitive awareness, conceptual understanding, and biology problem-solving abilities of grade XI students of SMA Negeri 13 Bone. The novelty of this study lies in integration of PjBL-STEM which simultaneously measures three high-level cognitive aspects in one experimental design. This study is a quasi-experimental study with a Pretest-Posttest Nonequivalent Control Group Design. The sample consisted of two classes (n=72) using a purposive sampling technique. Instruments included the MAI questionnaire, an essay test based on the SOLO taxonomy (prestructural to expanded abstract), and a problem-solving test based on Polya's stages, all of which have met high validity and reliability. Data were analyzed using ANCOVA (α = 0.05). The results showed that PjBL-STEM had a significant effect on metacognitive awareness (p=.004), conceptual understanding (p=.01), and biology problem-solving ability (p<.001). The experimental group showed a higher average increase compared to the control, indicating the effectiveness of PjBL-STEM in enhancing systematic and reflective thinking to support development of 21st century skills. Limitations of this study include quasi-experimental design without full randomization and sample size limited to one school. Further research is needed to test the generalizability of the findings.