Background: Back pain during pregnancy is a problem that often occurs during third trimester pregnancy with varying incidences of 50% in the UK, Australia reaches 70% while in Indonesia as many as 60-80% of 180 pregnant women who have been studied who experience spinal pain. From the data obtained 65% of third trimester pregnant women experience low back pain. Prenatal yoga because this sport is a combination of pregnant gymnastics and relaxation movements that can stretch muscles and maintain the flexibility of the spine and joints so that pregnant women become relaxed and facilitate the mother's adaptation to changes in her body during pregnancy Purpose: To determine the Effect of Prenatal Yoga on Back Pain in Trimester III Pregnant Women in the Ambacang Padang Health Center Working Area. Method: Quasi-Experiment research with pre-experimental design with One-Group Pre-Posttest design approach. The sample in this study were third trimester pregnant women. Purposive sampling technique will be used to determine the sample. This research will be conducted within eight months. Results: The results showed that before prenatal yoga was done almost half of pregnant women experienced back pain on a scale of 4-6 (moderate pain) as many as 25 respondents (62.5%) While the results of the study after prenatal yoga were 5.08 with a standard deviation of 1.256 and showed that the average back pain score before prenatal yoga intervention and after prenatal yoga intervention was 9.69 and 5.08. Conclusion: Based on statistical tests using the Paired Sample T-Test test, the p value is 0.000 (p <0.005), meaning that Ho is rejected, so there is a difference in pain scores before and after prenatal yoga in third trimester pregnant women.