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Sogandi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Library & Information Science

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Molecular Identification of Endophytic Bacteria Isolated From Allium cepa L. Waste as Antibiofilm Agent Against S.mutans Ekajayanti Kining; Dian Firdiani; Sogandi; Muh. Achyar Ardat
Jurnal Biodjati Vol 10 No 1 (2025): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i1.44587

Abstract

Streptococcus mutans is a key contributor to dental caries due to its ability to form resilient biofilms. Endophytic bacteria are a promising source of natural antibiofilm agents. This study aimed to isolate and characterize endophytic bacteria from red onion (Allium cepa L) waste with potential antibiofilm activity against S. mutans. This research employed an exploratory laboratory design with descriptive and quantitative approaches. Morphological and Gram-staining analyses were conducted alongside molecular identification through 16S rRNA sequencing. Antibacterial and antibiofilm assays were performed to evaluate the biological activity of isolate EA1. Absorbance values from biofilm inhibition and degradation tests were analyzed using descriptive statistics to calculate inhibition percentages. Isolate EA1 exhibited strong biofilm degradation (77% inhibition), moderate inhibition of biofilm formation (53%), and relatively lower antibacterial activity (30% growth inhibition). Molecular analysis confirmed its identity as a member of the Enterobacter genus, closely related to E. tabaci and E. asburiae. EA1 holds significant potential as a source of novel antibiofilm compounds for controlling S. mutans. Further investigation is needed to characterize its active metabolites and evaluate its clinical applicability.
Identification of Bioactive Compounds and Antibacterial Activity of Papaya Leaf Extract (Carica Papaya L.) as an Inhibitor of Pseudomonas Aeruginosa Wd Windi Pratiwi; Sogandi
BIOEDUKASI: Jurnal Biologi dan Pembelajarannya Vol. 23 No. 3 (2025): Vol. 23, No. 3, October 2025
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v23i3.53758

Abstract

This study explores the potential of papaya leaf extract (Carica papaya L.) as a natural antibacterial agent against Pseudomonas aeruginosa. The extraction process was carried out using 96% ethanol through maceration, followed by fractionation with n-hexane and ethyl acetate. Antibacterial activity was assessed using disc diffusion and liquid dilution methods to determine the inhibition zone and the Minimum Inhibitory Concentration (MIC).  Phytochemica creening confirmed the presence of alkaloids, flavonoids, saponins, and tannins in all tested fractions. Among them, the n-hexane fraction demonstrated the strongest antibacterial activity, with an inhibition zone of 15.10 mm at a 10% concentration and a MIC at 6%. Further nalysis using Gas Chromatography-Mass Spectrometry (GC-MS) identified 79 compounds in the n-hexane fraction, with several dominant compounds such as hexadecanoic acid methyl ester, pentadecanoic acid 14-methyl methyl ester, and phytol — all known for their antibacterial properties. These results suggest that the n-hexane fraction of papaya leaf extract holds promising potential for development as a natural antibacterial agent, particularly against Pseudomonas aeruginosa.
Co-Authors Dian Firdiani Ekajayanti Kining Muh. Achyar Ardat Wd Windi Pratiwi