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All Journal HAYATI Journal of Biosciences Jurnal Sumberdaya Hayati Smart: Journal of Sharia, Tradition, and Modernity Journal Indonesia Law and Policy Review (JILPR)
Miftahudin, M.
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Social Sciences

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Drought Tolerance in Transgenic Potato Cultivar IPB CP1 Expressing MmCuZn-SOD Gene Musawira; Tjahjoleksono, Aris; Hamim, Hamim; Miftahudin, M.
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1377-1386

Abstract

Drought stress induces reactive oxygen species (ROS) accumulation, which is harmful to plant cells. Consequently, it inhibits plant growth and decreases yield. The enzyme superoxide dismutase (SOD), which is encoded by the SOD gene, is the first defence enzyme in the cells that detoxify ROS. The study aimed to analyze the tolerance of transgenic potato cultivar IPB CP1 and its yield under drought-stress conditions. The results showed that transgenic plants had superior morphological characteristics, such as plant height, number of leaves, stem diameter, and plant biomass, than non-transgenic plants. However, photosynthetic rate, chlorophyll content, stomatal conductance, and transpiration rate showed similar levels between the transgenic and non-transgenic plants. The transgenic plants expressing the MmCu/Zn-SOD gene showed lower lipid peroxidation levels than the non-transgenic plants, indicating that the gene works well to reduce the cell's ROS level. Transgenic plant clone CP1S6 showed 13 times higher gene expression and tuber yield than non-transgenic plants. These research indicated that the transgenic plants expressing the MmCu/Zn-SOD gene are more tolerant to drought stress than the non-transgenic plants.
Regulation of the Director General of Islamic Guidance on Marriage of Husband during Wife's Iddah Period: An Effort to Develop Family Law in Indonesia Miftahudin, M.
SMART: Journal of Sharia, Traditon, and Modernity Vol. 3 No. 2 December (2023)
Publisher : Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/smart.v3i2.20509

Abstract

This research examines the husband's marriage during his wife's iddah period at the Office of Religious Affairs in Lampung Province. This type of research is field research, which is conducted at the KUA throughout Lampung Province, as a sample of Bandar Lampung City, Metro City and East Lampung Regency. Data sources in this research are primary and secondary data sources. Data analysis in this research uses qualitative methods and uses mashlahah theory. The result of the study is that the Circular Letter of the Director General of Islamic Guidance Number P-005/DJ.III/HK.00.7/10/2021 concerning Marriage of Husband in Wife's Iddah Period, is a regulation that regulates the prevention of husbands who want to marry during their wife's iddah period. The existence of this prevention is an effort to create balance between men and women in iddah arrangements, this will have an impact on the value of gender justice and create benefits between couples. Then by seeing that the existence of male iddah aims at gender justice, and kemashlahatan, it needs to be studied further to be regulated in legislation. The position of Circular Letter of the Director General of Islamic Guidance Number P-005/DJ.III/HK.00.7/10/2021 concerning Marriage during the Wife's Iddah Period has the status of a government policy, so according to the researcher's analysis, there should be a follow-up regulation to the legislative stage so that it becomes the status of a law, which can bind generally and widely, and has a higher legal force than a circular letter.Keyword: Marriage, Husband, Iddah Period, Wife, Lampung Province
Analysis of the Director General of Islamic Bimas Circular Letter Concerning Husband's Marriage During the Wife's Iddah Period Miftahudin, M.
JILPR Journal Indonesia Law and Policy Review Vol. 5 No. 2 (2024): Journal Indonesia Law and Policy Review (JILPR), February 2024
Publisher : International Peneliti Ekonomi, Sosial dan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56371/jirpl.v5i2.233

Abstract

Marriages are carried out with the aim of achieving peace, inner and outer happiness, but along the way the marriage does not turn out as expected. There are many breakdowns in marriages. Not a few marriages even occur among ex-husbands during the Iddah period. This is not permitted considering that the husband still has responsibilities towards his wife who is still in the Iddah period. Based on this, this research aims to analyze husbands' marriage problems during the wife's iddah period at the East Lampung Religious Affairs Office, and after studying the Circular Letter of the Director General of Islamic Community Guidance Number P-005/DJ.III/Hk.00.7/10/2021. The method used in this research is descriptive analytical qualitative with secondary data and primary data from documents from the East Lampung Religious Affairs office. Data collection techniques were carried out using interviews, documentation, literature documents. The research results stated that the Circular Letter of the Director General of Islamic Guidance Number P-005/DJ.III/HK.00.7/10/2021 concerning Husband's Marriage during the Wife's Iddah Period prohibits husbands from marrying. The aim of this circular is to create gender justice and benefit between couples in iddah arrangements.
Genetic Engineering of Potato (Solanum tuberosum L.) IPB CP2 Variety with OsGERLP Gene: Genetic Transformation of Potato (Solanum tuberosum L.) IPB CP2 Variety with OsGERLP Gene Nurhasanah, Eka; Tjahjoleksono, Aris; Miftahudin, M.
Jurnal Sumberdaya Hayati Vol. 11 No. 4 (2025): 2025
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.11.4.137-145

Abstract

High dissolved aluminum (Al) content in acidic soil can inhibit root elongation, especially in plants sensitive to Al. The OsGERLP gene is known to be an Al tolerance gene in rice, but the role of this gene in other plants is not yet known. Introduction of the OsGERLP gene to potato cv. IPB CP2 mediated by Agrobacterium tumefaciens has been succesfully conducted. When using leaf explant, the transformation and regeneration efficiencies were 72.62 and 17.24%, respectively. However, transgene introduction using internode explant increased transformation and regeneration efficiencies to 71.3 and 87.65%, respectively. PCR-based transgene integration analysis showed that all transgenic clones contain the OsGERLP gene under the control of the 35ScaMV promoter, which was indicated by the formation of 1500 bp amplicons. Transgenic clones have been in vitro tested for Al stress using liquid MS medium with different pH and Al content. The result showed that all transgenic clones have higher tolerance to Al compared to non-transgenic plant. Morphological analysis showed that transgenic clones performed better in all parameters including plant height, total root length, number of roots, shoot and root fresh weight, shoot and root dry weight. The transgenic clones can potentially be developed as potato transgenic lines tolerant to Al toxicity.
Introduction of the OsGERLP Gene into Potato cv. IPB CP3 to Develop Aluminum Stress-Tolerant Potato Lines Mahfudhah, Farah; Tjahjoleksono, Aris; Miftahudin, M.
HAYATI Journal of Biosciences Vol. 33 No. 3 (2026): May 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.3.646-656

Abstract

The OsGERLP gene is an aluminum (Al) stress tolerance gene. Potato cv. IPB CP3 is a horticultural crop that has not been proven to be tolerant to Al; therefore, enhancing its tolerance through genetic transformation is necessary. This research aims to obtain transgenic potatoes cv. IPB CP3 contains the OsGERLP gene and is tolerant to Al stress. Experimental methods include transforming potatoes with the OsGERLP gene via Agrobacterium tumefaciens, transgene integration testing, in vitro assays of transgenic plants under low pH and Al stress, and analysis of transgene expression. The results showed that the transformation efficiency achieved was relatively high at 47.03%, with a regeneration efficiency of 42.19%. The transgenic clones had longer roots and more roots than the non-transgenic ones under aluminum stress. The transgenic clones GERLP2, GERLP3, and GERLP4 exhibited the greatest root growth enhancement under stress conditions and the highest OsGERLP gene expression levels. These clones have the potential to be developed into Al-tolerant potato varieties. Future research is required to evaluate aluminum stress tolerance, tuber yield performance, and transgene stability across the three clones under greenhouse and field conditions of the three clones.
Co-Authors Aris Tjahjoleksono Hamim Hamim Mahfudhah, Farah Musawira Nurhasanah, Eka