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Hubungan antara Kurang Energi Kronik dengan Siklus Menstruasi Nurhasanah, Eka; Setiadi, Diding Kelana; Prameswari, Ayu
Jurnal Keperawatan Florence Nightingale Vol 7 No 1 (2024)
Publisher : Sekolah Tinggi Ilmu Kesehatan Stella Maris Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52774/jkfn.v7i1.170

Abstract

Chronic energy deficiency (CED) hurts young women even into the next phase of life, one of which is hormonal imbalance which can affect the menstrual cycle. Riskesdas data show that women aged 15-19 years are in first place with the highest proportion of KEK. This study aims to identify the relationship between CED and the menstrual cycle among female students at SMKN 2 Sumedang. The methodology applied is a quantitative approach with a correlational survey design with a descriptive retrospective approach. The research sample included 178 female students who were identified as having CED using total sampling. Data was collected through a menstrual cycles questionnaire which was proven to be valid (r count > r table) and reliable (P = 0.720), while CED was measured using a LILA measuring tape. Data analysis was carried out univariately and bivariately using the chi-square test for non-parametric nominal data. The results of the analysis show that there is a significant correlation between CED and the menstrual cycle where the p-value <0,001. Based on the findings, it can be concluded that there is a relationship between CED and the menstrual cycle of female students at SMKN 2 Sumedang, this shows the importance of maintaining nutritional balance as a preventive measure for CED and the impacts it can cause.
Genetic Engineering of Potato (Solanum tuberosum L.) IPB CP2 Variety with OsGERLP Gene: Genetic Transformation of Potato (Solanum tuberosum L.) IPB CP2 Variety with OsGERLP Gene Nurhasanah, Eka; Tjahjoleksono, Aris; Miftahudin, M.
Jurnal Sumberdaya Hayati Vol. 11 No. 4 (2025): 2025
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.11.4.137-145

Abstract

High dissolved aluminum (Al) content in acidic soil can inhibit root elongation, especially in plants sensitive to Al. The OsGERLP gene is known to be an Al tolerance gene in rice, but the role of this gene in other plants is not yet known. Introduction of the OsGERLP gene to potato cv. IPB CP2 mediated by Agrobacterium tumefaciens has been succesfully conducted. When using leaf explant, the transformation and regeneration efficiencies were 72.62 and 17.24%, respectively. However, transgene introduction using internode explant increased transformation and regeneration efficiencies to 71.3 and 87.65%, respectively. PCR-based transgene integration analysis showed that all transgenic clones contain the OsGERLP gene under the control of the 35ScaMV promoter, which was indicated by the formation of 1500 bp amplicons. Transgenic clones have been in vitro tested for Al stress using liquid MS medium with different pH and Al content. The result showed that all transgenic clones have higher tolerance to Al compared to non-transgenic plant. Morphological analysis showed that transgenic clones performed better in all parameters including plant height, total root length, number of roots, shoot and root fresh weight, shoot and root dry weight. The transgenic clones can potentially be developed as potato transgenic lines tolerant to Al toxicity.