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All Journal Jurnal Sain Veteriner Jurnal Ilmu Komunikasi dan Sosial Politik
Sumarningsih, Sumarningsih
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Environmental Science Library & Information Science Veterinary

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Tantangan BP3MI Provinsi Kepulauan Riau Dalam Menangani Pemulangan Pekerja Migran Indonesia Sumarningsih, Sumarningsih; Della Julianti; Rofiah Adawiyah; Maureen Azizta; Riszky Safutra; Kustiawan, Kustiawan
Jurnal Ilmu Komunikasi Dan Sosial Politik Vol. 2 No. 4 (2025): April - Juni
Publisher : CV. ITTC INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62379/jiksp.v2i4.2616

Abstract

Artikel ilmiah ini bertujuan untuk melihat bagaimana tantangan yang dihadapi oleh BP3MI Provinsi Kepulauan Riau dalam menangani proses pemulangan Pekerja Migran Indonesia (PMI), terutama PMI berstatus non-prosedural dan ilegal. Pendekatan yang dilakukan adalah deskriptif kualitatif dengan wawancara mendalam terhadap pejabat BP3MI, mantan PMI, serta pihak terkait lainnya. Hasil penelitian ini menunjukkan bahwa BP3MI menghadapi kendala berupa maraknya PMI ilegal tanpa dokumen dan pelatihan, perbedaan sistem hukum antara Indonesia dan negara tujuan PMI, serta keterbatasan aksesibilitas dan koordinasi antarlembaga. Meskipun regulasi telah tersedia, implementasinya masih lemah karena kurangnya sosialisasi dan pengawasan. Penelitian ini menekankan perlunya pendekatan terpadu dari berbagai pihak untuk memperkuat perlindungan dan pemberdayaan bagi PMI.
Development of In-House ELISA using recombinant LipL32 for Detection of Human Leptospirosis in Indonesia Sumarningsih, Sumarningsih; Sekarmila, Gita; Mulyadi, Andi; Ahpas, Ahpas; Tarigan, Simson
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.90085

Abstract

Early laboratory confirmation is important for the accurate diagnosis and treatment of patient infected by leptospirosis. However, Microscopic agglutination test (MAT) as the gold standard for detection of human leptospirosis has many limitation and only available in reference laboratories. Therefore, many studies suggested LipL32 protein as a good candidate for development of leptospirosis detection kit because it is highly conserved and produced only in pathogenic Leptospira species. In this study, we aim to investigate the performance of our in-house ELISA using recombinant LipL32 to detect leptospirosis in Indonesia. Fourteen human sera were used in this study and the infection status were determine using MAT. The result showed that nine of eleven MAT positive sera were successfully recognized by LipL32 ELISA. The antibody binding to LipL32 was also confirm by immunoblot. There was one of three MAT negative sera has high OD above 0.5 in ELISA, but it showed negative reaction in immunoblot result. Overall, this study demonstrated that recombinant LipL32 protein can recognized antibody from human leptospirosis and can be used as a universal antigen to detect infection by any serovars of pathogenic leptospira.
Co-Authors Ahpas, Ahpas Andi Mulyadi Della Julianti Kustiawan, Kustiawan Maureen Azizta Riszky Safutra Rofiah Adawiyah sekarmila, Gita Simson Tarigan