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Ahpas, Ahpas
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Detection of Very Virulent Infectious Bursal Disease (IBD) in Chicken in West Java Putri, Ajeng Fabeana; Winarsongko, Agus; Hoerudin, Heri; sekarmila, Gita; Ahpas, Ahpas; Jaelani, Jejen; Gunawan, Wawan; Dewiyanti, Rina; Pratama, Yuda; Nuradji, Harimurti; Fairusya, Nuha; Ekawasti, Fitrine; Adji, Rahmat Setya; Dharmayanti, NLP Indi; Indriani, Risa; Utomo, Bambang Ngaji; Suryatmiati, Sri
Jurnal Medika Veterinaria Vol 18, No 1 (2024): J.Med.Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v18i1.35819

Abstract

Infectious Bursal Disease (IBD), also known as Gumboro disease, is an acute, highly contagious disease that infects chickens and causes a high mortality rate of up to 100% in young animals. The disease is caused by Infectious Bursal Disease Virus (IBDV) of the genus Avibirnavirus, family Birnaviridae. The disease has been reported in Indonesia since 1976, and management strategies for the disease, such as vaccination, have been applied to prevent and control outbreaks in poultry farms. In this study, we conducted the detection of the disease in chickens from a farm in West Java with a mortality rate of 80%. Chickens showing clinical signs, such as sudden death, anorexia, watery diarrhea, and ruffled feathers, were necropsied, and organ samples, including the bursa Fabricius, brain, and spleen, were collected. The samples were then tested using Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) to confirm the diagnosis of IBD. Positive results were obtained in this study, highlighting the need for improved biosecurity in poultry farms in Indonesia. These results also provided a basis for further research on viral characterization to develop detection kits or vaccines for IBD using local isolates from the field in Indonesia.
Development of In-House ELISA using recombinant LipL32 for Detection of Human Leptospirosis in Indonesia Sumarningsih, Sumarningsih; Sekarmila, Gita; Mulyadi, Andi; Ahpas, Ahpas; Tarigan, Simson
Jurnal Sain Veteriner Vol 42, No 1 (2024): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.90085

Abstract

Early laboratory confirmation is important for the accurate diagnosis and treatment of patient infected by leptospirosis. However, Microscopic agglutination test (MAT) as the gold standard for detection of human leptospirosis has many limitation and only available in reference laboratories. Therefore, many studies suggested LipL32 protein as a good candidate for development of leptospirosis detection kit because it is highly conserved and produced only in pathogenic Leptospira species. In this study, we aim to investigate the performance of our in-house ELISA using recombinant LipL32 to detect leptospirosis in Indonesia. Fourteen human sera were used in this study and the infection status were determine using MAT. The result showed that nine of eleven MAT positive sera were successfully recognized by LipL32 ELISA. The antibody binding to LipL32 was also confirm by immunoblot. There was one of three MAT negative sera has high OD above 0.5 in ELISA, but it showed negative reaction in immunoblot result. Overall, this study demonstrated that recombinant LipL32 protein can recognized antibody from human leptospirosis and can be used as a universal antigen to detect infection by any serovars of pathogenic leptospira.
Co-Authors Andi Mulyadi Bambang Ngaji Utomo Dewiyanti, Rina Fairusya, Nuha Fitrine Ekawasti, Fitrine Hoerudin, Heri Jejen Jaelani NLP Indi Dharmayanti Nuradji, Harimurti Putri, Ajeng Fabeana Rahmat Setya Adji RISA INDRIANI sekarmila, Gita Simson Tarigan Sumarningsih, Sumarningsih Suryatmiati, Sri Wawan Gunawan Winarsongko, Agus yuda pratama