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All Journal Biosel: Biologi Science and Education Bionature
Halifah Pagarra
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Energy Environmental Science Immunology & microbiology

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Phytochemical Screening and Antioxidant Activity of the Centella asiatica Leaf Extracts In a Variety of Solvents Erlin Saalino; Halifah Pagarra
Bionature Vol. 24 No. 2 (2023): Oktober 2023
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

Centella asiatica is a type of herbaceous plant that has many health benefits. This plant is a source of phenolic compounds, flavonoids, triterpenoids, alkaloids and tannins. This research aims to examine the phytochemical content and antioxidant potential of 70% ethanol extract of Centella asiatica leaf and the results of fractionation using 3 types of solvents with different polarities. Centella asiatica leaves were extracted by maceration method, then fractionation and phytochemical screening were carried out. The total phenolic content test was carried out using the Folin Ciocalteu method and the antioxidant activity test was carried out using the DPPH (2,2-diphenyl1-picrylhydrazyl) method. The yield of 70% ethanol solvent maceration, n-hexane fraction, ethyl acetate fraction and methanol fraction were 18.782%, 3.05%, 6.19%, and 92.46%, respectively. Phytochemical screening of 70% ethanol extract of Centella asiatica leaves contains flavonoids, tannins, phenolics, alkaloids and steroids. The nhexane fraction contains alkaloids, tannins, phenolics and triterpenoids. The ethyl acetate fraction contains flavonoids, saponins, tannins, phenolics, alkaloids and steroids. The methanol:water fraction contains alkaloids, flavonoids, saponins, tannins, phenolics and triterpenoids. The total phenolic content test of 70% ethanol extract, n-hexane fraction, ethyl acetate fraction and methanol:water fraction was 12.773 ± 0.479 mg GAE/g, 1.182 ± 0.124 mg GAE/g, 18.334 ± 0.305 mg GAE/g, and 10.211 ± 0.247 mgGAE/g, respectively. Antioxidant activity of 70% ethanol extract, n-hexane fraction, ethyl acetate fraction and methanol:water fraction with IC50 values of 7.4 ppm, 2122.5 ppm, 45.6 ppm and 126 ppm.
Laju Pertumbuhan Jamur Rhizopus sp. pada Tempe Kacang Hijau (Phaseolus radiatus L.) Halifah Pagarra
Bionature Vol. 10 No. 2 (2009): Oktober 2009
Publisher : Jurusan Biologi Universitas Negeri Makassar

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This research was a descriptive study, which aims to determine the rate of growth of the fungus Rhizopus sp. the green bean tempe. This research was conducted by growing the fungus on PDA medium using fungus inoculum of 1 gram of green beans with tempeh making dilutions, starting from the dilutions 10-2, 10-3and 10-4are plated on a petri dish of 0.1 mL and incubated for 24 hours. Variation of time of fermentation used without fermentation (0 h), 12 hours, 24 hours, 36 hours, 48 hours, 60 hours and 72 hours for each serial dilution. The number of fungi indirectly calculated using the count method bowls. The results of this study indicate that optimum growth is shown in 36 hours fermentation time with the number of colonies on the 10-2dilution of 69 colonies (6.9 x 103 colonies / g) and 10-3dilutions of the 27 colonies. While the 10-4dilution only at the time of fermentation 36, 48 and 60 hours of growth of 2 colonies and each colony 1. The longer the fermentation time decreased the amount of mushrooms.
The English Halifah Pagarra; Rachmawaty; Musawira; Handayani, Besti; Syaiful Haq, Muhammad Naufal
Biosel Biology Science and Education Vol. 14 No. 2 (2025): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v14i2.9376

Abstract

Matoa (Pometia pinnata) is a plant commonly used by the Indonesian people for traditional medicine due to its rich content of metabolites. This study aimed to analyze the phytochemical composition and antibacterial activity of matoa leaves The leaves were extracted using a 70% ethanol solvent with a ratio of 1:4 using the maceration method, and phytochemical screening was performed on the leaf extract. The antibacterial activity was tested against Staphylococcus aureus and Escherichia coli using the disc diffusion method. The results indicated that the matoa leaf extract contains various metabolites, including triterpenoids, steroids, flavonoids, phenolics, saponins, tannins, and alkaloids. Secondary metabolite compounds in matoa leaves are thought to be able to inhibit microbial growth so that inhibition zones can be formed. Furthermore, the antibacterial activity demonstrated that the matoa leaf extract effectively inhibited the growth of S. aureus and E. coli. the largest inhibition zone is of 18.22 mm at 10% extract concentration, and E. coli, with an inhibition zone largest is of 10.87 mm at 10% extract concentration. While the smallest inhibition zone in S. aureus is 15.29 mm at 7.5% concentration, and in E. coli is 5% at 5% extract concentration.. This study suggests that matoa leaves have the potential to serve as natural bioactive compounds and antimicrobial agents in the clinical industry. Keywords: Matoa, Phytochemical,  Antibacterial activity, Staphylococcus aureus, Escherichia coli
Sintesis Nanopartikel Ekstrak Kulit Batang Tanaman Kayu Jawa (Lannea Coromandelica) Tersalut Kitosan Hartati; Fitratul Insaniah Rusli; Andi Mu'nisa; Halifah Pagarra
Bionature Vol. 23 No. 2 (2022): Oktober 2022
Publisher : Jurusan Biologi Universitas Negeri Makassar

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This study aims to synthesize nanoparticles of Javanese bark extract (Lannea coromandelica) coated with chitosan and characterization of the synthesis results. The extraction of Javanese bark (Lannea coromandelica) was carried out by the maceration method using a 70% ethanol solvent. The bark extract of the Javanese wood plant (Lannea coromandelica) was synthesized to form chitosan-coated nanoparticles using the ionic glassing method and the synthesis results were characterized using X-Ray Difraction (XRD). The results showed that the synthesis of nanoparticles of the bark extract of the Javanese wood plant (Lannea coromandelica) coated in chitosan has a nanoparticle size of 24.9 nm.
Co-Authors Andi Mu'nisa Erlin Saalino Fitratul Insaniah Rusli Handayani, Besti HARTATI Musawira Rachmawaty Syaiful Haq, Muhammad Naufal