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All Journal Jurnal Mahasiswa Ilmu Kesehatan Nian Tana Sikka: Jurnal Ilmiah Mahasiswa Vitalitas Medis : Jurnal Kesehatan Dan Kedokteran
Azzahra Nabilla Atha
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Agriculture, Biological Sciences & Forestry Humanities Computer Science & IT Education Health Professions Languange, Linguistic, Communication & Media Nursing Public Health Social Sciences

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Efektivitas Penggunaan Pewarna Metilen Blue dalam Pengamatan Bentuk Sel pada kulit katak Azzahra Nabilla Atha; Erika Dwi Susanti; Azizah Zahra Nur Fitria; Erlanda Islami Pasha; Elsa Trisna Sari; Nur Aini Fatna Fadilla; David Valentino Iskandar; Liss Dyah Dewi Arini
Nian Tana Sikka : Jurnal ilmiah Mahasiswa Vol. 3 No. 4 (2025): Nian Tana Sikka : Jurnal ilmiah Mahasiswa
Publisher : Fakultas Ekonomi & Bisnis, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59603/niantanasikka.v3i4.928

Abstract

This study aims to evaluate the effectiveness of using Methylene Blue stain in the microscopic observation of frog skin cell structures. Cell staining is an essential technique in histological studies, as it enhances the visibility of cellular components that are otherwise difficult to observe without contrast. Methylene Blue is known to have a high affinity for acidic components within the cell nucleus, thus providing a clearer depiction of nuclear and cell membrane structures. This research employed a descriptive experimental method by preparing frog skin tissue samples stained with Methylene Blue and observing them under a light microscope. The results indicated that Methylene Blue staining produced high contrast, allowing for the identification of hexagonal-shaped cells and dark, centralized nuclei. The obtained microscopic images revealed a uniform distribution of cells with clearly observable morphological structures. Therefore, Methylene Blue is proven to be effective in enhancing the microscopic visualization of epithelial tissue in frog skin and is recommended for use in both educational and basic biological research contexts.
Efektivitas Penggunaan Pewarna Metilen Blue dalam Pengamatan Bentuk Sel pada Kulit Katak Azzahra Nabilla Atha; Erika Dwi Susanti; Azizah Zahra Nur Fitria; Erlanda Islami Pasha; Elsa Trisna Sari; Nur Aini Fatna Fadilla; David Valentino Iskandar; Liss Dyah Dewi Arini
Jurnal Mahasiswa Ilmu Kesehatan Vol. 3 No. 3 (2025): Jurnal Mahasiswa Ilmu Kesehatan
Publisher : STIKes Ibnu Sina Ajibarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59841/jumkes.v3i3.3021

Abstract

This study aims to evaluate the effectiveness of using Methylene Blue stain in the microscopic observation of frog skin cell structures. Cell staining is an essential technique in histological studies, as it enhances the visibility of cellular components that are otherwise difficult to observe without contrast. Methylene Blue is known to have a high affinity for acidic components within the cell nucleus, thus providing a clearer depiction of nuclear and cell membrane structures. This research employed a descriptive experimental method by preparing frog skin tissue samples stained with Methylene Blue and observing them under a light microscope. The results indicated that Methylene Blue staining produced high contrast, allowing for the identification of hexagonal-shaped cells and dark, centralized nuclei. The obtained microscopic images revealed a uniform distribution of cells with clearly observable morphological structures. Therefore, Methylene Blue is proven to be effective in enhancing the microscopic visualization of epithelial tissue in frog skin and is recommended for use in both educational and basic biological research contexts.
Analisis Resiko Infeksi Jamur pada Wanita Hijab dan Non-Hijab Akibat Kelembapan Rambut Erika Dwi Susanti; Azzahra Nabilla Atha; Erlanda Islami Pasha; Elsa Trisna Sari; Azizah Zahra Nur Fitria; Nur Aini Fatna Fadilla; Liss Dyah Dewi Arini
Vitalitas Medis : Jurnal Kesehatan dan Kedokteran Vol. 3 No. 3 (2026): Juli: Vitalitas Medis : Jurnal Kesehatan dan Kedokteran
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/vimed.v3i3.3282

Abstract

Hair and scalp health is an important aspect of dermatology that is influenced by various environmental and physiological factors, as well as daily habits. One factor that is often considered is the moisture level of the scalp, which can create a distinct microecological environment conducive to the growth of microorganisms. This study employed a quantitative approach with a comparative observational analytical design to analyze the relationship between hair moisture levels and the risk of fungal infection by the genus Malassezia sp. in two groups: women who wear the hijab and women who do not. The study was conducted in May 2026 in Surakarta, Solo, after obtaining informed consent from the participants. Based on macroscopic observations on PDA medium, differences in fungal colony growth were observed in each group, both in the hijab and non-hijab samples. Some samples showed more visible colony growth, while others showed only slight growth or no dominant colonies. These differences indicate that the number of microorganisms in each sample was not the same. Microscopic observations using LPCB staining revealed fungal structures consisting of round cells in several preparations. This study suggests that wearing a hijab can increase scalp temperature and humidity, potentially supporting the growth of Malassezia sp. However, the results of both macroscopic and microscopic cultures indicate that fungal growth varies among individuals and is not necessarily higher among hijab wearers.
Co-Authors Azizah Zahra Nur Fitria David Valentino Iskandar Elsa Trisna Sari Erika Dwi Susanti Erlanda Islami Pasha Liss Dyah Dewi Arini Nur Aini Fatna Fadilla