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All Journal Jurnal Mahasiswa Ilmu Kesehatan Nian Tana Sikka: Jurnal Ilmiah Mahasiswa Jurnal Riset Ilmu Kesehatan Umum dan Farmasi
Azizah Zahra Nur Fitria
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Humanities Computer Science & IT Education Health Professions Languange, Linguistic, Communication & Media Nursing Public Health Social Sciences

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Efektivitas Penggunaan Pewarna Metilen Blue dalam Pengamatan Bentuk Sel pada kulit katak Azzahra Nabilla Atha; Erika Dwi Susanti; Azizah Zahra Nur Fitria; Erlanda Islami Pasha; Elsa Trisna Sari; Nur Aini Fatna Fadilla; David Valentino Iskandar; Liss Dyah Dewi Arini
Nian Tana Sikka : Jurnal ilmiah Mahasiswa Vol. 3 No. 4 (2025): Nian Tana Sikka : Jurnal ilmiah Mahasiswa
Publisher : Fakultas Ekonomi & Bisnis, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59603/niantanasikka.v3i4.928

Abstract

This study aims to evaluate the effectiveness of using Methylene Blue stain in the microscopic observation of frog skin cell structures. Cell staining is an essential technique in histological studies, as it enhances the visibility of cellular components that are otherwise difficult to observe without contrast. Methylene Blue is known to have a high affinity for acidic components within the cell nucleus, thus providing a clearer depiction of nuclear and cell membrane structures. This research employed a descriptive experimental method by preparing frog skin tissue samples stained with Methylene Blue and observing them under a light microscope. The results indicated that Methylene Blue staining produced high contrast, allowing for the identification of hexagonal-shaped cells and dark, centralized nuclei. The obtained microscopic images revealed a uniform distribution of cells with clearly observable morphological structures. Therefore, Methylene Blue is proven to be effective in enhancing the microscopic visualization of epithelial tissue in frog skin and is recommended for use in both educational and basic biological research contexts.
Efektivitas Penggunaan Pewarna Metilen Blue dalam Pengamatan Bentuk Sel pada Kulit Katak Azzahra Nabilla Atha; Erika Dwi Susanti; Azizah Zahra Nur Fitria; Erlanda Islami Pasha; Elsa Trisna Sari; Nur Aini Fatna Fadilla; David Valentino Iskandar; Liss Dyah Dewi Arini
Jurnal Mahasiswa Ilmu Kesehatan Vol. 3 No. 3 (2025): Jurnal Mahasiswa Ilmu Kesehatan
Publisher : STIKes Ibnu Sina Ajibarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59841/jumkes.v3i3.3021

Abstract

This study aims to evaluate the effectiveness of using Methylene Blue stain in the microscopic observation of frog skin cell structures. Cell staining is an essential technique in histological studies, as it enhances the visibility of cellular components that are otherwise difficult to observe without contrast. Methylene Blue is known to have a high affinity for acidic components within the cell nucleus, thus providing a clearer depiction of nuclear and cell membrane structures. This research employed a descriptive experimental method by preparing frog skin tissue samples stained with Methylene Blue and observing them under a light microscope. The results indicated that Methylene Blue staining produced high contrast, allowing for the identification of hexagonal-shaped cells and dark, centralized nuclei. The obtained microscopic images revealed a uniform distribution of cells with clearly observable morphological structures. Therefore, Methylene Blue is proven to be effective in enhancing the microscopic visualization of epithelial tissue in frog skin and is recommended for use in both educational and basic biological research contexts.
Hubungan antara Kerusakan DNA dan Mekanisme Perbaikan Seluler: Tinjauan Literatur Azizah Zahra Nur Fitria; Margaretha Alesandria Natasya Weka; Azlinatul Lulu Kharolaini; Desi Rahmasari; Pramai Sheila Eka Khoireina; Feni Atika Tsuroyya; Septi Puspita Kurniawati; Tyara Avrilyanti Ghea Indriansyah; Liss Dyah Dewi Arini
Jurnal Riset Ilmu Kesehatan Umum dan Farmasi (JRIKUF) Vol. 4 No. 2 (2026): April : Jurnal Riset Ilmu Kesehatan Umum dan Farmasi (JRIKUF)
Publisher : LPPM STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/jrikuf.v4i2.972

Abstract

DNA damage is a fundamental biological phenomenon that occurs due to endogenous and exogenous factors such as radiation, chemical exposure, and replication errors. If not properly repaired, DNA damage can lead to genomic instability, mutations, and the development of various diseases, including cancer. This literature review aims to examine the relationship between DNA damage and cellular repair mechanisms in maintaining genomic integrity. The method used in this study is a literature review of national scientific journals that discuss types of DNA damage, molecular markers of DNA damage, and cellular repair pathways. The findings indicate that cells possess complex and coordinated DNA repair systems, including base excision repair, nucleotide excision repair, mismatch repair, and double-strand break repair mechanisms, which play crucial roles in preserving cellular stability. The effectiveness of these repair mechanisms is influenced by the type and extent of DNA damage. Understanding the interaction between DNA damage and repair pathways provides important implications for the development of disease prevention strategies, diagnostic biomarkers, and therapeutic approaches in molecular biology and medical sciences.
Co-Authors Azlinatul Lulu Kharolaini Azzahra Nabilla Atha David Valentino Iskandar Desi Rahmasari Elsa Trisna Sari Erika Dwi Susanti Erlanda Islami Pasha Feni Atika Tsuroyya Liss Dyah Dewi Arini Margaretha Alesandria Natasya Weka Nur Aini Fatna Fadilla Pramai Sheila Eka Khoireina Septi Puspita Kurniawati Tyara Avrilyanti Ghea Indriansyah